##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062498_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876868
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.895958114562283	32.0	32.0	32.0	32.0	32.0
2	30.431771942869393	32.0	32.0	32.0	32.0	32.0
3	30.48957767873842	32.0	32.0	32.0	32.0	32.0
4	30.308331470643243	32.0	32.0	32.0	21.0	32.0
5	30.31330029149199	32.0	32.0	32.0	21.0	32.0
6	33.60368493319405	36.0	36.0	36.0	21.0	36.0
7	33.61209783000406	36.0	36.0	36.0	21.0	36.0
8	33.51273737894415	36.0	36.0	36.0	21.0	36.0
9	33.766646747286934	36.0	36.0	36.0	32.0	36.0
10	33.30363521077289	36.0	36.0	36.0	21.0	36.0
11	33.835783721153014	36.0	36.0	36.0	32.0	36.0
12	33.49787083118554	36.0	36.0	36.0	27.0	36.0
13	33.692441735814285	36.0	36.0	36.0	32.0	36.0
14	33.512056546709424	36.0	36.0	36.0	27.0	36.0
15	33.38694535551531	36.0	36.0	36.0	21.0	36.0
16	33.43471309250651	36.0	36.0	36.0	27.0	36.0
17	33.33463873695927	36.0	36.0	36.0	21.0	36.0
18	33.396466742998946	36.0	36.0	36.0	27.0	36.0
19	33.3336716586761	36.0	36.0	36.0	21.0	36.0
20	33.293040685713244	36.0	36.0	36.0	21.0	36.0
21	33.25778338358795	36.0	36.0	36.0	21.0	36.0
22	33.20239990511685	36.0	36.0	36.0	21.0	36.0
23	33.19303703636123	36.0	36.0	36.0	21.0	36.0
24	33.178528581268786	36.0	36.0	36.0	21.0	36.0
25	32.42433296687757	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	46.0
5	81.0
6	140.0
7	52.0
8	124.0
9	146.0
10	96.0
11	44.0
12	50.0
13	41.0
14	438.0
15	754.0
16	971.0
17	1167.0
18	1408.0
19	1667.0
20	2139.0
21	2780.0
22	3674.0
23	5015.0
24	6798.0
25	9140.0
26	12665.0
27	17304.0
28	23332.0
29	31144.0
30	42876.0
31	59873.0
32	89602.0
33	125366.0
34	227015.0
35	210917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.810828504203364	17.817465160122584	11.07907531617923	26.292631019494824
2	16.55486469718811	20.13838202288196	37.40203547251063	25.904717807419303
3	18.917800017107176	23.865879736549484	28.751347190146266	28.464973056197074
4	12.36126144724941	15.410287440161783	36.501056756913975	35.72739435567483
5	14.188681225537003	36.68648233644657	34.148776094339475	14.976060343676952
6	33.701097702480524	35.86284480761968	16.962076153466132	13.47398133643366
7	29.6204452863778	30.770178822079448	21.121318285042623	18.48805760650013
8	27.53525427017526	33.28619447916132	19.953888384780885	19.224662865882543
9	26.856667336353006	14.494333540327295	18.904446412916425	39.744552710403276
10	15.243909400838948	26.848187719927203	32.495221828195895	25.412681051037957
11	36.807895316722636	21.346766210620178	22.756921474982903	19.088416997674287
12	23.98371662822299	24.029963231095987	29.2914563683285	22.694863772352527
13	28.946893682321186	19.76997766684783	25.763506076535574	25.519622574295408
14	22.72041688503121	20.169690658792984	25.593580482034735	31.51631197414107
15	24.653244358691868	27.732916240916538	22.808980425959437	24.804858974432157
16	25.178385907227913	26.16353278304849	24.394515418250734	24.26356589147287
17	23.23856500194104	26.272407572332213	26.01516293302277	24.47386449270398
18	24.224787613044672	25.11726957157212	27.054900886087513	23.6030419292957
19	24.746961482422044	25.63156861043487	25.853319874485585	23.7681500326575
20	24.80573157504139	24.99503339612947	25.913227150767824	24.286007878061312
21	25.750390795830448	24.843082200149126	24.880648840972075	24.525878163048358
22	24.759567341130573	25.348528852490094	25.37182692274748	24.52007688363185
23	23.582794146867315	24.96899630239511	26.031914966506868	25.41629458423071
24	23.978400450352208	25.71963291270772	25.787802352016936	24.514164284923133
25	24.06642794504531	25.237389469453376	25.927748648056127	24.76843393744519
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	87.0
2	260.5
3	434.0
4	434.0
5	434.0
6	1185.5
7	1937.0
8	1937.0
9	1937.0
10	2146.5
11	2356.0
12	2356.0
13	2356.0
14	2741.5
15	3127.0
16	3127.0
17	3127.0
18	4965.0
19	6803.0
20	6803.0
21	6803.0
22	11008.5
23	15214.0
24	15214.0
25	15214.0
26	22000.0
27	28786.0
28	28786.0
29	28786.0
30	35954.0
31	43122.0
32	43122.0
33	43122.0
34	51942.0
35	60762.0
36	60762.0
37	60762.0
38	67998.5
39	75235.0
40	75235.0
41	75235.0
42	83916.0
43	92597.0
44	92597.0
45	92597.0
46	105501.0
47	118405.0
48	118405.0
49	118405.0
50	116500.5
51	114596.0
52	114596.0
53	114596.0
54	105575.0
55	96554.0
56	96554.0
57	96554.0
58	87089.5
59	77625.0
60	77625.0
61	77625.0
62	68218.5
63	58812.0
64	58812.0
65	58812.0
66	48419.0
67	38026.0
68	38026.0
69	38026.0
70	28703.5
71	19381.0
72	19381.0
73	19381.0
74	15213.0
75	11045.0
76	11045.0
77	11045.0
78	9051.5
79	7058.0
80	7058.0
81	7058.0
82	4949.5
83	2841.0
84	2841.0
85	2841.0
86	2025.0
87	1209.0
88	1209.0
89	1209.0
90	811.0
91	413.0
92	413.0
93	413.0
94	241.0
95	69.0
96	69.0
97	69.0
98	221.5
99	374.0
100	374.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008096999776477189
2	0.0022808450074583634
3	0.004903816766035481
4	0.014711450298106443
5	0.03295821035777335
6	0.04573094239954018
7	0.0659164207155467
8	0.08359296952334902
9	0.09533932131175958
10	0.11518267287664735
11	0.11484054612552859
12	0.1286396584206517
13	0.11860394038783488
14	0.11848989813746195
15	0.10982268710912019
16	0.1092524758572556
17	0.11951627839081823
18	0.12635881341319333
19	0.12681498241468497
20	0.11609501087963069
21	0.1244200951568537
22	0.14357919321950396
23	0.1329732639348226
24	0.12647285566356622
25	0.12453413740722663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	876868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.88029266615793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.19164899765309	48.98348640362099
2	10.524673883182926	12.39391756915359
3	2.6975453612371862	4.764967810496467
4	1.1152611647490693	2.626676151185015
5	0.595646269211457	1.7535913328337842
6	0.36394904648174087	1.285765582344841
7	0.2449993410487633	1.0097943032776917
8	0.18077157652574447	0.8515106665246878
9	0.12998145289250415	0.6888011388734759
>10	0.7622742462936197	8.622820075913658
>50	0.09894516406259088	4.138416954363101
>100	0.08792641596147519	10.051375464449922
>500	0.005604101221118847	2.19817657512566
>1k	7.729794787750135E-4	0.630699971837131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1642	0.18725737511233162	No Hit
GTACATGGAAGCAGTGGTATCAACG	1510	0.17220379806310643	No Hit
TCCATGTACTCTGCGTTGATACCAC	1310	0.14939534798852278	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1081	0.12327967265312453	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	969	0.1105069406113577	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	897	0.10229589858450759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.1404225037291816E-4	0.0
8	0.0	0.0	0.0	1.1404225037291816E-4	0.0
9	0.0	0.0	0.0	1.1404225037291816E-4	0.0
10	0.0	0.0	0.0	1.1404225037291816E-4	0.0
11	0.0	0.0	0.0	1.1404225037291816E-4	0.0
12	0.0	0.0	0.0	1.1404225037291816E-4	3.421267511187545E-4
13	0.0	0.0	0.0	1.1404225037291816E-4	3.421267511187545E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	50	8.741016E-5	15.193057	2
CCCTTAA	65	3.3941087E-6	14.604538	1
CTCGTAG	60	2.5643523E-5	14.250813	10
CAAGACG	160	0.0	14.247559	4
CGCATCG	115	2.5465852E-11	14.04428	13
GCGTTAT	55	1.9709859E-4	13.807928	1
GCATCGC	120	5.0931703E-11	13.459101	14
GCCGTTT	85	2.690722E-7	13.413298	15
TATTCCC	85	2.6963426E-7	13.410998	5
GAACCGC	65	5.444265E-5	13.154597	6
ACGCCCC	80	1.9888867E-6	13.066978	17
AAGACGG	175	0.0	13.027827	5
CCGTCGT	90	5.392194E-7	12.667391	9
AACCGCG	60	4.086885E-4	12.667389	7
CGCAAGA	195	0.0	12.660881	2
CTAGGAC	90	5.425918E-7	12.660881	3
CGCGGTC	70	1.0892701E-4	12.214984	10
TTAACCT	55	0.0030705428	12.088839	4
GTGCTAG	55	0.0030837026	12.081937	1
TAGGACA	175	0.0	11.940811	4
>>END_MODULE