FastQCFastQC Report
Thu 2 Feb 2017
SRR4062498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876868
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9970.1137001236217994No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9790.11164736311508687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8870.10115547608077839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA300.0057395829.3381252
GTATTCG450.001396578424.4484371
CGTCGTA1600.023.37481910
CCGTCGT1750.022.6283979
ACTATAC500.00257999521.9998283
GTCGTAG1800.021.99982811
ATACCGT1950.021.435736
GATATAC1750.021.374921
TCAATAC956.862392E-720.8419423
ACCGTCG1800.020.7776178
CTATACT856.1106875E-620.705724
TACCGTC1950.020.3075337
TCTAGCG2900.019.72398428
CTAGCGG2900.019.72398429
CGCAATA2800.019.64270436
CAATACG2900.018.96536838
GCTTATA708.1119814E-418.8602222
TAGCGGC3050.018.75395230
CGGTCCA3050.018.75395210
CGGCTAA1303.5843186E-818.615249
Produced by FastQC (version 0.11.5)