##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876868
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.982024660496222	32.0	32.0	32.0	32.0	32.0
2	30.8886240574408	32.0	32.0	32.0	32.0	32.0
3	31.19891819521296	32.0	32.0	32.0	32.0	32.0
4	31.424424200677866	32.0	32.0	32.0	32.0	32.0
5	31.26745302599707	32.0	32.0	32.0	32.0	32.0
6	34.849035430646346	36.0	36.0	36.0	32.0	36.0
7	34.875192161191876	36.0	36.0	36.0	32.0	36.0
8	34.78253169234138	36.0	36.0	36.0	32.0	36.0
9	34.99660154093889	36.0	36.0	36.0	32.0	36.0
10	34.784774903406216	36.0	36.0	36.0	32.0	36.0
11	35.01814070076682	36.0	36.0	36.0	36.0	36.0
12	34.881312808769394	36.0	36.0	36.0	32.0	36.0
13	34.955404918414175	36.0	36.0	36.0	32.0	36.0
14	34.90228061692296	36.0	36.0	36.0	32.0	36.0
15	34.853420355173185	36.0	36.0	36.0	32.0	36.0
16	34.87196134423881	36.0	36.0	36.0	32.0	36.0
17	34.83993143779907	36.0	36.0	36.0	32.0	36.0
18	34.8581645127887	36.0	36.0	36.0	32.0	36.0
19	34.845540035672414	36.0	36.0	36.0	32.0	36.0
20	34.83132923085345	36.0	36.0	36.0	32.0	36.0
21	34.82985466455612	36.0	36.0	36.0	32.0	36.0
22	34.80583280493757	36.0	36.0	36.0	32.0	36.0
23	34.76105069406113	36.0	36.0	36.0	32.0	36.0
24	34.74250286246048	36.0	36.0	36.0	32.0	36.0
25	34.717104512879935	36.0	36.0	36.0	32.0	36.0
26	34.661846480884236	36.0	36.0	36.0	32.0	36.0
27	34.633332497023495	36.0	36.0	36.0	32.0	36.0
28	34.62205942057413	36.0	36.0	36.0	32.0	36.0
29	34.59699407436467	36.0	36.0	36.0	32.0	36.0
30	34.58663333591829	36.0	36.0	36.0	32.0	36.0
31	34.576399184369826	36.0	36.0	36.0	32.0	36.0
32	34.54752596742041	36.0	36.0	36.0	32.0	36.0
33	34.52046830309693	36.0	36.0	36.0	32.0	36.0
34	34.50641373616097	36.0	36.0	36.0	32.0	36.0
35	34.478931834666106	36.0	36.0	36.0	32.0	36.0
36	34.4319270403299	36.0	36.0	36.0	32.0	36.0
37	34.43671681484556	36.0	36.0	36.0	32.0	36.0
38	34.39417449376645	36.0	36.0	36.0	32.0	36.0
39	34.384710127408	36.0	36.0	36.0	32.0	36.0
40	34.36400803769781	36.0	36.0	36.0	32.0	36.0
41	34.331582404649275	36.0	36.0	36.0	32.0	36.0
42	34.306586624212535	36.0	36.0	36.0	32.0	36.0
43	34.289022977232605	36.0	36.0	36.0	32.0	36.0
44	34.25616968574518	36.0	36.0	36.0	32.0	36.0
45	34.194648453359	36.0	36.0	36.0	32.0	36.0
46	34.20273518933294	36.0	36.0	36.0	32.0	36.0
47	34.17967812715255	36.0	36.0	36.0	32.0	36.0
48	34.128766245318566	36.0	36.0	36.0	32.0	36.0
49	34.12993631880739	36.0	36.0	36.0	32.0	36.0
50	33.49497073675855	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	17.0
22	63.0
23	204.0
24	520.0
25	1300.0
26	2842.0
27	5376.0
28	9450.0
29	15466.0
30	23558.0
31	35960.0
32	56815.0
33	97424.0
34	202541.0
35	425327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.546168532995125	18.364078899044898	11.724222882730398	26.365529685229582
2	16.25474580504478	20.80296341239492	36.63037292419143	26.311917858368865
3	18.62453171240727	24.121044858672658	29.06536353942739	28.18905988949268
4	12.436563853542046	16.02240251075991	36.73628581790148	34.80474781779656
5	14.506402331935936	36.52830300569755	33.72548661828234	15.239808044084171
6	34.003028969078514	36.36062978835991	16.459299368432575	13.177041874129
7	29.84668159859865	30.87728141521871	20.798227327260204	18.477809658922435
8	27.71762682638664	33.496945948535014	19.515594137315993	19.269833087762354
9	27.161670855818663	14.172942791845522	18.538023967119337	40.12736238521647
10	15.239922086334545	26.953999917889583	32.32949543146745	25.476582564308426
11	37.32716896955984	21.29510941213501	22.545924814225174	18.831796804079975
12	24.07922287048906	23.977155056405298	29.167788082128666	22.775833990976977
13	29.189798236450642	19.73546759603498	25.560403618332522	25.514330549181864
14	22.927396141722586	20.130053782325273	25.275640119151344	31.666909956800794
15	24.86246504605026	27.716258319382163	22.469060337473827	24.952216297093745
16	25.423096748883527	26.117043842402733	24.215959528686188	24.243899880027552
17	23.624194291501116	26.2982569782453	25.64365446110475	24.433894269148833
18	24.45715888822491	25.175282938823173	26.67106109471437	23.696497078237545
19	25.118261813636718	25.756442246723566	25.548543224293734	23.57675271534598
20	25.12978008092438	25.032273956855537	25.52539264746803	24.31255331475205
21	26.047135942924132	24.89576538315915	24.562876054320604	24.49422261959611
22	24.978217930178772	25.493574859613986	24.975138789418704	24.553068420788534
23	23.899720368402086	25.009807633532073	25.696570065277786	25.39390193278806
24	24.33330900431992	25.725080627871012	25.419447396871593	24.522162970937472
25	24.4369734099089	25.19900372690074	25.60738902548616	24.756633837704193
26	23.94442493054827	25.801374893370493	26.003343718780936	24.2508564573003
27	24.829392793442114	25.636127672580137	25.252945711327136	24.281533822650616
28	23.943284508044542	25.255682725336083	25.959209367886615	24.841823398732764
29	23.960618930101223	25.637040010583124	25.717325754845653	24.68501530447
30	24.20273062764293	25.554131294562012	25.973122522432114	24.27001555536295
31	24.430358959387274	25.388085778019043	25.154527249255302	25.02702801333838
32	24.041931054617113	25.585720997915306	25.38021686274331	24.99213108472427
33	23.9358717617703	25.178362079583245	25.845395202014444	25.040370956632014
34	24.344941313857955	25.355697778913132	26.218883571985753	24.08047733524316
35	25.254428260581978	25.393445763786566	25.401428721312673	23.950697254318783
36	23.987304816688486	25.744923979435903	25.555499801566484	24.712271402309128
37	25.089751251043484	25.430851621908886	25.132631137183704	24.346765989863925
38	24.237969683008163	25.459704311253233	25.563596801342964	24.738729204395646
39	24.628222263784284	25.30540514649868	25.108454180104644	24.957918409612393
40	24.9987455352459	25.466204719524487	25.520830957453118	24.014218787776496
41	23.99289288695676	25.565649561849675	26.140080376978066	24.301377174215503
42	25.159431066021337	26.011782845308527	25.331292737333328	23.497493351336804
43	24.212652303425372	25.035353097615605	25.839236920494308	24.91275767846472
44	24.130541883156873	25.34851311713964	25.70592152980836	24.815023469895124
45	24.192352782858993	25.761232021239227	25.55812277332506	24.488292422576716
46	24.197142557374658	25.40063042556006	25.460616649256217	24.941610367809066
47	24.479967338299495	25.038204153874926	25.898424848437852	24.583403659387727
48	24.83201576520069	26.008589662298085	24.925530410506486	24.233864161994738
49	24.135445699922908	25.441913720195057	25.66520844642523	24.757432133456803
50	23.819662708640298	26.05203975969017	25.15829064351761	24.97000688815192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	23.0
2	22.0
3	57.0
4	92.0
5	100.5
6	109.0
7	107.5
8	106.0
9	164.5
10	223.0
11	295.0
12	367.0
13	589.0
14	811.0
15	1109.0
16	1407.0
17	1706.5
18	2006.0
19	2265.5
20	2525.0
21	3312.5
22	4100.0
23	4532.5
24	4965.0
25	6296.5
26	7628.0
27	9582.5
28	11537.0
29	13024.0
30	14511.0
31	15435.5
32	16360.0
33	19178.0
34	21996.0
35	25333.0
36	28670.0
37	33279.5
38	37889.0
39	40033.0
40	42177.0
41	45530.0
42	48883.0
43	48997.5
44	49112.0
45	53996.0
46	58880.0
47	62051.5
48	65223.0
49	65411.0
50	65599.0
51	62725.5
52	59852.0
53	58219.0
54	56586.0
55	56779.0
56	56972.0
57	55349.0
58	53726.0
59	48772.5
60	43819.0
61	38738.5
62	33658.0
63	29572.0
64	25486.0
65	21664.0
66	17842.0
67	15673.0
68	13504.0
69	12400.5
70	11297.0
71	8860.5
72	6424.0
73	5690.5
74	4957.0
75	3625.5
76	2294.0
77	2027.5
78	1761.0
79	1542.0
80	1323.0
81	1080.0
82	837.0
83	734.0
84	631.0
85	509.5
86	388.0
87	280.5
88	173.0
89	120.5
90	68.0
91	46.5
92	25.0
93	17.5
94	10.0
95	9.5
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011632309538037651
2	0.004675732265289645
3	3.421267511187545E-4
4	2.2808450074583632E-4
5	0.0
6	2.2808450074583632E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	876868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.4548125568541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.95567517792931	52.434359563217036
2	10.253047745545768	12.807043501690728
3	2.549482471837411	4.776823496867717
4	1.0393374303048064	2.596464975720364
5	0.5477257274655603	1.7104053820714065
6	0.32645630587007024	1.223326043467096
7	0.23135131085498364	1.0114301927961344
8	0.15628414985380648	0.7808557827781426
9	0.11234512138827381	0.6314844148183495
>10	0.66846811657933	7.9760851852002155
>50	0.07973229444926232	3.488700642616391
>100	0.07587866648357988	8.798262950257097
>500	0.004215481437864991	1.7647578684993472
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	997	0.1137001236217994	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	979	0.11164736311508687	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	887	0.10115547608077839	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1404225037291816E-4
2	0.0	0.0	0.0	0.0	1.1404225037291816E-4
3	0.0	0.0	0.0	0.0	1.1404225037291816E-4
4	0.0	0.0	0.0	0.0	1.1404225037291816E-4
5	0.0	0.0	0.0	0.0	2.2808450074583632E-4
6	0.0	0.0	0.0	0.0	2.2808450074583632E-4
7	0.0	0.0	0.0	0.0	2.2808450074583632E-4
8	0.0	0.0	0.0	0.0	2.2808450074583632E-4
9	0.0	0.0	0.0	0.0	2.2808450074583632E-4
10	0.0	0.0	0.0	0.0	2.2808450074583632E-4
11	0.0	0.0	0.0	0.0	2.2808450074583632E-4
12	0.0	0.0	0.0	0.0	2.2808450074583632E-4
13	0.0	0.0	0.0	0.0	2.2808450074583632E-4
14	0.0	0.0	0.0	0.0	2.2808450074583632E-4
15	0.0	0.0	0.0	1.1404225037291816E-4	2.2808450074583632E-4
16	0.0	0.0	0.0	1.1404225037291816E-4	2.2808450074583632E-4
17	0.0	0.0	0.0	1.1404225037291816E-4	2.2808450074583632E-4
18	0.0	0.0	0.0	1.1404225037291816E-4	2.2808450074583632E-4
19	0.0	0.0	0.0	1.1404225037291816E-4	2.2808450074583632E-4
20	0.0	0.0	0.0	2.2808450074583632E-4	2.2808450074583632E-4
21	0.0	0.0	0.0	3.421267511187545E-4	2.2808450074583632E-4
22	0.0	0.0	0.0	3.421267511187545E-4	2.2808450074583632E-4
23	0.0	0.0	0.0	5.702112518645908E-4	2.2808450074583632E-4
24	0.0	0.0	0.0	0.001368507004475018	2.2808450074583632E-4
25	0.0	0.0	0.0	0.0019387182563396087	2.2808450074583632E-4
26	0.0	0.0	0.0	0.002737014008950036	2.2808450074583632E-4
27	0.0	0.0	0.0	0.003421267511187545	3.421267511187545E-4
28	0.0	0.0	0.0	0.005930197019391744	3.421267511187545E-4
29	0.0	0.0	0.0	0.00992167578244388	3.421267511187545E-4
30	0.0	0.0	0.0	0.017448464307056478	3.421267511187545E-4
31	0.0	0.0	0.0	0.03637947786896089	3.421267511187545E-4
32	0.0	0.0	0.0	0.06021430819690079	3.421267511187545E-4
33	0.0	0.0	0.0	0.08655806803304489	3.421267511187545E-4
34	0.0	0.0	0.0	0.11472650387515566	4.5616900149167265E-4
35	0.0	0.0	0.0	0.1488251367366582	4.5616900149167265E-4
36	0.0	0.0	0.0	0.20037223390521722	4.5616900149167265E-4
37	1.1404225037291816E-4	0.0	0.0	0.2832809499263287	4.5616900149167265E-4
38	1.1404225037291816E-4	0.0	0.0	0.3936738482873135	4.5616900149167265E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATA	30	0.00573958	29.338125	2
GTATTCG	45	0.0013965784	24.448437	1
CGTCGTA	160	0.0	23.374819	10
CCGTCGT	175	0.0	22.628397	9
ACTATAC	50	0.002579995	21.999828	3
GTCGTAG	180	0.0	21.999828	11
ATACCGT	195	0.0	21.43573	6
GATATAC	175	0.0	21.37492	1
TCAATAC	95	6.862392E-7	20.841942	3
ACCGTCG	180	0.0	20.777617	8
CTATACT	85	6.1106875E-6	20.70572	4
TACCGTC	195	0.0	20.307533	7
TCTAGCG	290	0.0	19.723984	28
CTAGCGG	290	0.0	19.723984	29
CGCAATA	280	0.0	19.642704	36
CAATACG	290	0.0	18.965368	38
GCTTATA	70	8.1119814E-4	18.860222	2
TAGCGGC	305	0.0	18.753952	30
CGGTCCA	305	0.0	18.753952	10
CGGCTAA	130	3.5843186E-8	18.61524	9
>>END_MODULE