FastQCFastQC Report
Thu 2 Feb 2017
SRR4062496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569442
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG51970.9126478201467402No Hit
TCCATGTACTCTGCGTTGATACCAC41840.73475437357975No Hit
GAGTACATGGAAGCAGTGGTATCAA23920.4200603397712146No Hit
CATGTACTCTGCGTTGATACCACTG19600.34419659947808556No Hit
GTATCAACGCAGAGTACTTTTTTTT16600.29151344649674593No Hit
GCTTCCATGTACTCTGCGTTGATAC14970.26288893337688474No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13850.24322055626385128No Hit
CCCATGTACTCTGCGTTGATACCAC13650.2397083460650953No Hit
GTACATGGGAAGCAGTGGTATCAAC12530.22003996895206185No Hit
GCGTTGATACCACTGCTTCCATGTA11930.20950333835579393No Hit
CATGGAAGCAGTGGTATCAACGCAG11720.20581551764710013No Hit
TATCAACGCAGAGTACTTTTTTTTT11220.19703499215021023No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10060.17666417299742554No Hit
GTATCAACGCAGAGTACATGGAAGC8660.1520787016061337No Hit
ACTCTGCGTTGATACCACTGCTTCC7710.13539570316204283No Hit
CAGTGGTATCAACGCAGAGTACATG7260.1274932302148419No Hit
ACGCAGAGTACATGGAAGCAGTGGT7140.1253859040955883No Hit
GTACATGGGGTGGTATCAACGCAAA7020.12327857797633474No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6800.11941514675770316No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6230.10940534769124863No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG6120.10747363208193284No Hit
GGTATCAACGCAGAGTACTTTTTTT5880.10325897984342566No Hit
GAATAACGCCGCCGCATCGCCAGTC5850.10273214831361228No Hit
GAATAGGACCGCGGTTCTATTTTGT5810.10202970627386108No Hit
GTCCTATTCCATTATTCCTAGCTGC5760.10115165372417208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC350.002171479616.281723
GTCTAGG350.002176586816.2759971
CCAACGA551.12466405E-515.5471119
TCCAACG807.668859E-915.43914318
GCCCCTT807.677954E-915.4377856
GCATCGC1004.0017767E-1115.20161914
GTCGCGT456.7475555E-414.779358
TCGCGTA456.7475555E-414.779359
ATCGAAC456.7475555E-414.779358
TCCCCGC801.2929195E-714.2414961
CGAACCC551.9549468E-413.819653510
CGCATCG1101.8553692E-1013.819653513
CATCGCC1101.8553692E-1013.819653515
CCGCATC1101.8553692E-1013.819653512
TCCATTA707.235225E-613.5728748
ACGGTAT852.685374E-713.4131939
CGCCCCT852.691122E-713.4108345
AACCGCG500.001496307213.3014177
CAAATCG500.001496307213.30141713
GAACCGC500.001497299413.3002466
Produced by FastQC (version 0.11.5)