##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062496_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569442
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02007754960119	32.0	32.0	32.0	32.0	32.0
2	30.653687293877162	32.0	32.0	32.0	32.0	32.0
3	30.656895697893727	32.0	32.0	32.0	32.0	32.0
4	30.70549941872921	32.0	32.0	32.0	32.0	32.0
5	30.490815570330252	32.0	32.0	32.0	32.0	32.0
6	34.123533213215744	36.0	36.0	36.0	32.0	36.0
7	33.96433701764183	36.0	36.0	36.0	32.0	36.0
8	33.93656245939007	36.0	36.0	36.0	32.0	36.0
9	34.114085367781094	36.0	36.0	36.0	32.0	36.0
10	33.75495660664299	36.0	36.0	36.0	32.0	36.0
11	34.17172249324778	36.0	36.0	36.0	32.0	36.0
12	33.89518159882833	36.0	36.0	36.0	32.0	36.0
13	34.02668928530035	36.0	36.0	36.0	32.0	36.0
14	33.924069527713094	36.0	36.0	36.0	32.0	36.0
15	33.85680719019672	36.0	36.0	36.0	32.0	36.0
16	33.85894612620776	36.0	36.0	36.0	32.0	36.0
17	33.76402513337618	36.0	36.0	36.0	32.0	36.0
18	33.78365312007193	36.0	36.0	36.0	32.0	36.0
19	33.77224546134637	36.0	36.0	36.0	32.0	36.0
20	33.731263236642185	36.0	36.0	36.0	27.0	36.0
21	33.68397659463123	36.0	36.0	36.0	27.0	36.0
22	33.63351667070571	36.0	36.0	36.0	27.0	36.0
23	33.56382739594199	36.0	36.0	36.0	27.0	36.0
24	33.55069875421904	36.0	36.0	36.0	27.0	36.0
25	33.01324278855441	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	18.0
5	59.0
6	98.0
7	23.0
8	78.0
9	93.0
10	72.0
11	11.0
12	61.0
13	30.0
14	121.0
15	174.0
16	272.0
17	364.0
18	495.0
19	737.0
20	1196.0
21	1506.0
22	2291.0
23	3221.0
24	4556.0
25	5930.0
26	8065.0
27	10302.0
28	13541.0
29	17819.0
30	22643.0
31	30746.0
32	43687.0
33	60960.0
34	126560.0
35	213707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.37504851589653	16.71862184997919	10.760781944534695	25.145547689589588
2	15.681691258855471	20.629339927154202	37.684704841049346	26.004263972940983
3	19.916229814809935	25.00601494516302	28.06389013285565	27.013865107171398
4	12.12791294023423	15.361566401337651	36.05213609762609	36.458384560802024
5	13.633936131713847	37.65147549162558	33.94895596538506	14.765632411275503
6	33.83868020661302	34.989107136582454	17.564742260796233	13.607470396008292
7	29.17290484926442	30.88515417975806	21.34301921008793	18.598921760889585
8	28.34290957090605	33.096461332681834	20.29324554480853	18.26738355160359
9	27.33063807347513	14.321849182633152	18.225347161188257	40.12216558270346
10	15.333967435735596	27.33766656176433	32.65797325166188	24.670392750838193
11	37.46044136883413	21.223645160836604	21.645428077134056	19.67048539319521
12	23.559066028263917	24.00991013010123	30.015772510114978	22.415251331519876
13	30.361930884124096	19.685277492066003	25.353974910110683	24.59881671369922
14	23.24244299720085	20.059640189610793	25.327563895179555	31.370352918008805
15	25.318870263095437	27.366848041068554	24.03196174435879	23.28231995147722
16	25.443690236968823	25.711794292987626	25.4744562761841	23.37005919385945
17	23.335923791305206	26.096168114861612	26.936250118680483	23.631657975152702
18	24.83453839553236	24.40796044395054	28.304484519405253	22.453016641111844
19	24.634648557497023	26.940965255785578	25.29440002532095	23.129986161396452
20	24.250874265731067	24.457111309983244	27.560161262248073	23.731853162037613
21	25.611733179535513	25.537002911842897	24.246543065734503	24.604720842887083
22	24.132806413090695	25.927430630668656	25.44113741608584	24.498625540154805
23	23.070172816145217	25.427380112865983	25.57545296752421	25.92699410346459
24	23.230504379447808	27.085227342666073	25.680216783106115	24.004051494780008
25	24.681376341665846	25.301039571229623	25.11060390788752	24.906980179217015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	157.0
2	307.5
3	458.0
4	458.0
5	458.0
6	1249.0
7	2040.0
8	2040.0
9	2040.0
10	2056.0
11	2072.0
12	2072.0
13	2072.0
14	1977.5
15	1883.0
16	1883.0
17	1883.0
18	2959.0
19	4035.0
20	4035.0
21	4035.0
22	6522.0
23	9009.0
24	9009.0
25	9009.0
26	13532.0
27	18055.0
28	18055.0
29	18055.0
30	23169.5
31	28284.0
32	28284.0
33	28284.0
34	32666.0
35	37048.0
36	37048.0
37	37048.0
38	41308.0
39	45568.0
40	45568.0
41	45568.0
42	53203.0
43	60838.0
44	60838.0
45	60838.0
46	76133.0
47	91428.0
48	91428.0
49	91428.0
50	83894.5
51	76361.0
52	76361.0
53	76361.0
54	67277.0
55	58193.0
56	58193.0
57	58193.0
58	52975.5
59	47758.0
60	47758.0
61	47758.0
62	41867.0
63	35976.0
64	35976.0
65	35976.0
66	29808.0
67	23640.0
68	23640.0
69	23640.0
70	17862.0
71	12084.0
72	12084.0
73	12084.0
74	9445.5
75	6807.0
76	6807.0
77	6807.0
78	5665.5
79	4524.0
80	4524.0
81	4524.0
82	3101.0
83	1678.0
84	1678.0
85	1678.0
86	1265.5
87	853.0
88	853.0
89	853.0
90	588.0
91	323.0
92	323.0
93	323.0
94	193.0
95	63.0
96	63.0
97	63.0
98	185.0
99	307.0
100	307.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0072000309074497495
2	0.0035122101987559748
3	0.004741483768320566
4	0.015102503854650694
5	0.03652698606706214
6	0.046009953603703274
7	0.06427344663723435
8	0.08762964445896158
9	0.09518089638628692
10	0.11572732604900939
11	0.11695659961857398
12	0.1283712827645309
13	0.12064442032726774
14	0.12257613593658354
15	0.11186389483037781
16	0.11116145279062661
17	0.12082003083720555
18	0.1281956722545931
19	0.13047860888378446
20	0.11923953624776536
21	0.1281956722545931
22	0.15014698599681794
23	0.1410152394800524
24	0.13399081908254043
25	0.13100544041359788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	569442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.18758130531719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06748554613739	43.38663382943629
2	12.51506616979143	13.563223312339955
3	3.4992101691270228	5.6884120663188975
4	1.355277967172118	2.937569401497766
5	0.6517522949234043	1.765844023604452
6	0.3643177766412282	1.1844899485631364
7	0.2525518118603767	0.9579620287292531
8	0.1788666129117519	0.7753879303969803
9	0.11883527506002275	0.5795456516329244
>10	0.7813555117114543	8.201710760876859
>50	0.11056721224244181	4.276075856234785
>100	0.09495772207162712	10.321720286252338
>500	0.005528360531567327	1.887986327498677
>1k	0.003902372139929878	3.5576400938837773
>5k	3.251976783274898E-4	0.9157984827338644
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5197	0.9126478201467402	No Hit
TCCATGTACTCTGCGTTGATACCAC	4184	0.73475437357975	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2392	0.4200603397712146	No Hit
CATGTACTCTGCGTTGATACCACTG	1960	0.34419659947808556	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1660	0.29151344649674593	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1497	0.26288893337688474	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1385	0.24322055626385128	No Hit
CCCATGTACTCTGCGTTGATACCAC	1365	0.2397083460650953	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1253	0.22003996895206185	No Hit
GCGTTGATACCACTGCTTCCATGTA	1193	0.20950333835579393	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1172	0.20581551764710013	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1122	0.19703499215021023	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1006	0.17666417299742554	No Hit
GTATCAACGCAGAGTACATGGAAGC	866	0.1520787016061337	No Hit
ACTCTGCGTTGATACCACTGCTTCC	771	0.13539570316204283	No Hit
CAGTGGTATCAACGCAGAGTACATG	726	0.1274932302148419	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	714	0.1253859040955883	No Hit
GTACATGGGGTGGTATCAACGCAAA	702	0.12327857797633474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	680	0.11941514675770316	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	623	0.10940534769124863	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	612	0.10747363208193284	No Hit
GGTATCAACGCAGAGTACTTTTTTT	588	0.10325897984342566	No Hit
GAATAACGCCGCCGCATCGCCAGTC	585	0.10273214831361228	No Hit
GAATAGGACCGCGGTTCTATTTTGT	581	0.10202970627386108	No Hit
GTCCTATTCCATTATTCCTAGCTGC	576	0.10115165372417208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	1.7561050993779875E-4	0.0	0.0	0.0	0.0
12	1.7561050993779875E-4	0.0	0.0	0.0	0.0
13	1.7561050993779875E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGAC	35	0.0021714796	16.28172	3
GTCTAGG	35	0.0021765868	16.275997	1
CCAACGA	55	1.12466405E-5	15.54711	19
TCCAACG	80	7.668859E-9	15.439143	18
GCCCCTT	80	7.677954E-9	15.437785	6
GCATCGC	100	4.0017767E-11	15.201619	14
GTCGCGT	45	6.7475555E-4	14.77935	8
TCGCGTA	45	6.7475555E-4	14.77935	9
ATCGAAC	45	6.7475555E-4	14.77935	8
TCCCCGC	80	1.2929195E-7	14.241496	1
CGAACCC	55	1.9549468E-4	13.8196535	10
CGCATCG	110	1.8553692E-10	13.8196535	13
CATCGCC	110	1.8553692E-10	13.8196535	15
CCGCATC	110	1.8553692E-10	13.8196535	12
TCCATTA	70	7.235225E-6	13.572874	8
ACGGTAT	85	2.685374E-7	13.413193	9
CGCCCCT	85	2.691122E-7	13.410834	5
AACCGCG	50	0.0014963072	13.301417	7
CAAATCG	50	0.0014963072	13.301417	13
GAACCGC	50	0.0014972994	13.300246	6
>>END_MODULE