FastQCFastQC Report
Thu 2 Feb 2017
SRR4062496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569442
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18580.3262843274644301No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16690.2930939410861861No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8600.15102503854650692No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7160.12573712511546392No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6860.12046880981732995No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.11748343114838737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6620.11625415757882279No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6190.10870290565149743No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG5850.10273214831361228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACCC407.027779E-427.4998343
GATATAC2550.025.8890191
GCTCTAA551.5893966E-424.006181
GTATAGA551.5893966E-424.006181
GTAATAG551.59224E-423.9998573
AATCCCG1800.023.22208419
TAGGACG2100.023.0474824
TTAACGA1650.022.6665312
CTAGACA1306.730261E-1121.9998684
TATTGGG500.002578867121.9998682
CGCAATA1700.021.99986836
GTTATGA500.002578867121.99986834
ATACACT3100.021.9998664
TACGCTA1650.021.3332049
TGTAGGA3300.021.3332042
TGGTCGG1650.021.33320437
GACGTGG1259.931682E-1021.1198737
CGAATGT2200.020.99987433
ATCCCGT2000.020.89987420
ACGCTAT1700.020.7057610
Produced by FastQC (version 0.11.5)