##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569442
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148519076569695	32.0	32.0	32.0	32.0	32.0
2	31.34167483255538	32.0	32.0	32.0	32.0	32.0
3	31.46688863835123	32.0	32.0	32.0	32.0	32.0
4	31.59319474151889	32.0	32.0	32.0	32.0	32.0
5	31.517027195043568	32.0	32.0	32.0	32.0	32.0
6	35.11139150255865	36.0	36.0	36.0	36.0	36.0
7	35.123427846909784	36.0	36.0	36.0	36.0	36.0
8	35.06660906641941	36.0	36.0	36.0	36.0	36.0
9	35.1894363253852	36.0	36.0	36.0	36.0	36.0
10	35.07422529423541	36.0	36.0	36.0	36.0	36.0
11	35.20817221069046	36.0	36.0	36.0	36.0	36.0
12	35.10062482219436	36.0	36.0	36.0	36.0	36.0
13	35.138577765602115	36.0	36.0	36.0	36.0	36.0
14	35.10044394336912	36.0	36.0	36.0	36.0	36.0
15	35.064619399341815	36.0	36.0	36.0	36.0	36.0
16	35.06993512947763	36.0	36.0	36.0	36.0	36.0
17	35.04442945901426	36.0	36.0	36.0	36.0	36.0
18	35.061470702898625	36.0	36.0	36.0	36.0	36.0
19	35.02921807664345	36.0	36.0	36.0	36.0	36.0
20	35.00937408902048	36.0	36.0	36.0	36.0	36.0
21	34.98067581948644	36.0	36.0	36.0	36.0	36.0
22	34.95618693387562	36.0	36.0	36.0	36.0	36.0
23	34.88728439419642	36.0	36.0	36.0	32.0	36.0
24	34.871295057266586	36.0	36.0	36.0	32.0	36.0
25	34.82566617846945	36.0	36.0	36.0	32.0	36.0
26	34.7716009707749	36.0	36.0	36.0	32.0	36.0
27	34.72914010557704	36.0	36.0	36.0	32.0	36.0
28	34.683330347954666	36.0	36.0	36.0	32.0	36.0
29	34.651620358175194	36.0	36.0	36.0	32.0	36.0
30	34.61287716747272	36.0	36.0	36.0	32.0	36.0
31	34.60633567597753	36.0	36.0	36.0	32.0	36.0
32	34.55201056472828	36.0	36.0	36.0	32.0	36.0
33	34.51513235764134	36.0	36.0	36.0	32.0	36.0
34	34.47867737188335	36.0	36.0	36.0	32.0	36.0
35	34.402249921853326	36.0	36.0	36.0	32.0	36.0
36	34.354427667787064	36.0	36.0	36.0	32.0	36.0
37	34.29706449471588	36.0	36.0	36.0	32.0	36.0
38	34.21685088209159	36.0	36.0	36.0	32.0	36.0
39	34.09895827845505	36.0	36.0	36.0	32.0	36.0
40	34.03067739998104	36.0	36.0	36.0	32.0	36.0
41	34.01173429427404	36.0	36.0	36.0	32.0	36.0
42	33.862481165772806	36.0	36.0	36.0	32.0	36.0
43	33.901394698669925	36.0	36.0	36.0	32.0	36.0
44	33.86658869560026	36.0	36.0	36.0	32.0	36.0
45	33.69839772970733	36.0	36.0	36.0	27.0	36.0
46	33.796205759322284	36.0	36.0	36.0	32.0	36.0
47	33.71120500419709	36.0	36.0	36.0	27.0	36.0
48	33.68354634888189	36.0	36.0	36.0	27.0	36.0
49	33.712667839744874	36.0	36.0	36.0	27.0	36.0
50	33.13762420053315	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	16.0
22	63.0
23	208.0
24	523.0
25	1346.0
26	2885.0
27	5540.0
28	8844.0
29	12618.0
30	17288.0
31	22949.0
32	33350.0
33	47273.0
34	98446.0
35	318091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.774503174589235	17.34177548672644	11.256048404801842	25.627672933882483
2	15.270706705279261	21.03256488654185	37.2196474639851	26.477080944193798
3	19.379633954881832	25.23330020125106	28.401511665888123	26.985554177978987
4	11.887664877186142	15.703525750782788	36.29905924954209	36.109750122488975
5	13.396623361114916	37.72113753463917	33.93567738241998	14.946561721825926
6	34.08617955566795	35.34285498534523	17.079090122032387	13.49187533695444
7	29.093568791905057	30.769244277731534	21.318589074918957	18.81859785544445
8	28.222540662613575	33.23218167960916	20.176945149813328	18.368332507963935
9	27.157814140860705	13.909757271153165	18.28649801033292	40.64593057765321
10	15.132533251850056	27.38786390887922	32.7366088205647	24.74299401870603
11	37.76223039396462	21.01039262997812	21.60167321693869	19.625703759118576
12	23.501252102935858	23.920434390157382	30.037475282820726	22.540838224086034
13	30.552014076938477	19.407946726795704	25.497592379908752	24.542446816357067
14	23.35409049560798	19.723343202643992	25.468967866788894	31.453598434959133
15	25.45948489925225	27.2154143881203	23.89180987703752	23.43329083558993
16	25.488460633391984	25.62543683114347	25.509885115604398	23.376217419860144
17	23.41572978459615	26.009321405867496	26.883334913827923	23.69161389570843
18	24.98744384853945	24.212825889203817	28.28277506752224	22.516955194734496
19	24.563344467039663	26.705968298790744	25.531836429346622	23.198850804822968
20	24.416358470221724	24.28991890306651	27.65672359959399	23.636999027117774
21	25.589261066096284	25.14847868615241	24.40792916574471	24.854331082006595
22	24.187537975772774	25.64159299805775	25.698315192767655	24.472553833401822
23	23.174265333431677	24.949160757373008	25.735720231384406	26.14085367781091
24	23.362695410594934	26.705968298790744	25.840384095307336	24.090952195306986
25	24.981297480691627	24.714018284566293	25.376421128051675	24.92826310669041
26	22.922966693710688	25.700071297867034	26.69595849972429	24.681003508697987
27	24.859072565774916	25.73730072597385	26.015467773715322	23.388158934535912
28	23.70267735783451	24.800594265965632	26.29381043196673	25.20291794423313
29	23.151787188159638	25.76399352348439	26.87297389374159	24.211245394614377
30	24.310114111709357	25.288089041552958	26.82854443472733	23.573252412010355
31	23.574832906599795	25.422431081655372	25.85987686191043	25.1428591498344
32	23.512139954552	25.850745115393664	26.02442390972215	24.612691020332182
33	23.063279491150986	25.194488639756113	26.54370418760822	25.198527681484684
34	23.62312579683269	26.04567278142462	27.144116521085554	23.187084900657133
35	24.874877511669318	25.591368392215536	26.418669504532506	23.115084591582637
36	23.466832442988046	26.65995834518704	25.97349686184019	23.89971234998472
37	24.9672486398966	25.88973064859986	26.08026805188237	23.06275265962117
38	24.102015657433064	25.754159334927877	25.458782457212497	24.68504255042656
39	24.157859799593286	25.325494080169708	26.291878716357413	24.22476740387959
40	25.294059798890846	25.66126137517078	25.446314111006917	23.598364714931456
41	22.906986137306344	25.74432514637136	27.206458252113475	24.142230464208822
42	25.564499984195056	26.398825516909536	25.856189041201738	22.18048545769367
43	24.169625703759117	24.573529876616057	26.03478492980848	25.222059489816345
44	23.507574081293615	25.739934883622915	26.734944032930485	24.017547002152984
45	24.107459583241138	25.884989164831534	25.864442735168815	24.14310851675851
46	23.278051144804916	25.113356584164848	26.94725713944528	24.661335131584956
47	24.485900232157093	24.88681902634509	26.997130524267615	23.6301502172302
48	24.336455688200026	27.018028174950214	24.73052567250045	23.914990464349312
49	23.066967311859678	25.94610162228989	27.1183017761247	23.86862928972573
50	23.214831361227304	27.37890777287239	24.470446507282567	24.935814358617733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	13.0
2	12.0
3	66.5
4	121.0
5	145.5
6	170.0
7	154.5
8	139.0
9	199.5
10	260.0
11	352.0
12	444.0
13	746.5
14	1049.0
15	1478.0
16	1907.0
17	2237.0
18	2567.0
19	2778.5
20	2990.0
21	3357.5
22	3725.0
23	4037.5
24	4350.0
25	4587.0
26	4824.0
27	6135.0
28	7446.0
29	8510.5
30	9575.0
31	10367.5
32	11160.0
33	12768.0
34	14376.0
35	16690.5
36	19005.0
37	21196.0
38	23387.0
39	24505.0
40	25623.0
41	27016.0
42	28409.0
43	29692.0
44	30975.0
45	35895.0
46	40815.0
47	44658.0
48	48501.0
49	47685.5
50	46870.0
51	42674.5
52	38479.0
53	36655.0
54	34831.0
55	34948.5
56	35066.0
57	33805.5
58	32545.0
59	29688.5
60	26832.0
61	23783.0
62	20734.0
63	17929.0
64	15124.0
65	13002.0
66	10880.0
67	9414.5
68	7949.0
69	7435.5
70	6922.0
71	5349.0
72	3776.0
73	3435.0
74	3094.0
75	2187.5
76	1281.0
77	1216.5
78	1152.0
79	975.5
80	799.0
81	645.0
82	491.0
83	425.0
84	359.0
85	308.0
86	257.0
87	182.0
88	107.0
89	73.5
90	40.0
91	21.5
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013522009265210503
2	0.004390262748444969
3	7.02442039751195E-4
4	5.268315298133962E-4
5	0.0
6	5.268315298133962E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	569442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.77921657924907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73049306379407	48.26005629410111
2	12.234103582953178	14.626902554766504
3	3.3948264903866487	6.088202040533866
4	1.25703480479221	3.0057822337331044
5	0.6280769688428339	1.8772974574447023
6	0.35234903140735824	1.2637889435993461
7	0.23987851121327805	1.0037824632168464
8	0.1618363723764367	0.7739561243752808
9	0.12238490172671833	0.6584466191716448
>10	0.7041886981300147	8.00732470258193
>50	0.09686393095841848	4.044841006950816
>100	0.07471515827636362	8.73563421232872
>500	0.0026578514801867377	1.0313355105475264
>1k	5.906336622637195E-4	0.6226498366486007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1858	0.3262843274644301	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1669	0.2930939410861861	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	860	0.15102503854650692	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	716	0.12573712511546392	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	686	0.12046880981732995	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669	0.11748343114838737	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	662	0.11625415757882279	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	619	0.10870290565149743	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	585	0.10273214831361228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	1.7561050993779875E-4	0.0
19	0.0	0.0	0.0	3.512210198755975E-4	0.0
20	0.0	0.0	0.0	0.0010536630596267925	0.0
21	0.0	0.0	0.0	0.00140488407950239	0.0
22	0.0	0.0	0.0	0.0038634312186315726	0.0
23	0.0	0.0	0.0	0.007902472947200943	0.0
24	0.0	0.0	0.0	0.012292735695645913	0.0
25	0.0	0.0	0.0	0.014575672324837297	0.0
26	0.0	0.0	0.0	0.018263493033531072	0.0
27	0.0	0.0	0.0	0.02265375578197604	0.0
28	0.0	0.0	0.0	0.03090744974905258	0.0
29	0.0	0.0	0.0	0.0424977434049473	0.0
30	0.0	0.0	0.0	0.06831248836580371	0.0
31	0.0	0.0	0.0	0.11449805247944479	0.0
32	0.0	0.0	0.0	0.1699909736197892	0.0
33	0.0	0.0	0.0	0.21986435844212404	0.0
34	0.0	0.0	0.0	0.2816792579402292	0.0
35	0.0	0.0	0.0	0.3510454093656597	0.0
36	0.0	0.0	0.0	0.4513190105401428	0.0
37	0.0	0.0	0.0	0.5928610815500086	0.0
38	0.0	0.0	0.0	0.7568812978319126	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACCC	40	7.027779E-4	27.499834	3
GATATAC	255	0.0	25.889019	1
GCTCTAA	55	1.5893966E-4	24.00618	1
GTATAGA	55	1.5893966E-4	24.00618	1
GTAATAG	55	1.59224E-4	23.999857	3
AATCCCG	180	0.0	23.222084	19
TAGGACG	210	0.0	23.047482	4
TTAACGA	165	0.0	22.66653	12
CTAGACA	130	6.730261E-11	21.999868	4
TATTGGG	50	0.0025788671	21.999868	2
CGCAATA	170	0.0	21.999868	36
GTTATGA	50	0.0025788671	21.999868	34
ATACACT	310	0.0	21.999866	4
TACGCTA	165	0.0	21.333204	9
TGTAGGA	330	0.0	21.333204	2
TGGTCGG	165	0.0	21.333204	37
GACGTGG	125	9.931682E-10	21.119873	7
CGAATGT	220	0.0	20.999874	33
ATCCCGT	200	0.0	20.899874	20
ACGCTAT	170	0.0	20.70576	10
>>END_MODULE