FastQCFastQC Report
Thu 2 Feb 2017
SRR4062495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373043
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44950.3273750348678082No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29400.21412293715491795No Hit
TATCAACGCAGAGTACTTTTTTTTT28750.20938892663958814No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22800.16605452269156903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18280.1331349418772755No Hit
GTACATGGAAGCAGTGGTATCAACG17860.1300760427750624No Hit
GCGCAAGACGGACCAGAGCGAAAGC15820.11521853285002728No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15480.11274228119585476No Hit
TCCATGTACTCTGCGTTGATACCAC15460.11259661933384461No Hit
GAATAACGCCGCCGCATCGCCAGTC15170.11048452233469745No Hit
GATTAAGAGGGACGGCCGGGGGCAT14900.10851808719756047No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14350.10451238599228138No Hit
GAATAGGACCGCGGTTCTATTTTGT14160.10312859830318498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG2500.013.6778995
CGCGTAT500.001497829113.3018397
ATAACGA1102.737579E-912.95680812
CAAGACG2600.012.78514
AGTCCGG604.0867133E-412.66841810
TACACCG751.4806179E-512.6647225
TTAGAGT1650.012.6633384
CGGTCCA2500.012.54173310
GAACAGT2450.012.4085216
AGGCCCG2950.012.23897910
GGTCCAA2800.012.21597411
GCGTTAT1405.2750693E-1112.206181
TAATACC550.003074177312.087734
GTTATTC1603.6379788E-1211.8710133
CGCCAGT2650.011.83311818
CTATAGC658.04008E-411.6892344
GAGTACG658.0671697E-411.6845481
ATATACG2050.011.5856996
TCGCCAG2800.011.53815117
ATCGCCA2650.011.47370216
Produced by FastQC (version 0.11.5)