##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062495_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1373043
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09688261766019	32.0	32.0	32.0	32.0	32.0
2	30.81372469762418	32.0	32.0	32.0	32.0	32.0
3	30.798154901193918	32.0	32.0	32.0	32.0	32.0
4	30.84900691384028	32.0	32.0	32.0	32.0	32.0
5	30.690166294864763	32.0	32.0	32.0	32.0	32.0
6	34.30451777548117	36.0	36.0	36.0	32.0	36.0
7	34.17372507634502	36.0	36.0	36.0	32.0	36.0
8	34.158280549116085	36.0	36.0	36.0	32.0	36.0
9	34.305394659890474	36.0	36.0	36.0	32.0	36.0
10	34.01244971934601	36.0	36.0	36.0	32.0	36.0
11	34.303785096315266	36.0	36.0	36.0	32.0	36.0
12	34.09774056602743	36.0	36.0	36.0	32.0	36.0
13	34.21367721185717	36.0	36.0	36.0	32.0	36.0
14	34.13250568263339	36.0	36.0	36.0	32.0	36.0
15	34.084767192287494	36.0	36.0	36.0	32.0	36.0
16	34.08135724809784	36.0	36.0	36.0	32.0	36.0
17	34.01032014292342	36.0	36.0	36.0	32.0	36.0
18	34.04068627129667	36.0	36.0	36.0	32.0	36.0
19	34.00251776528484	36.0	36.0	36.0	32.0	36.0
20	33.98679283897154	36.0	36.0	36.0	32.0	36.0
21	33.95882794639352	36.0	36.0	36.0	32.0	36.0
22	33.91929386042535	36.0	36.0	36.0	32.0	36.0
23	33.89613435267504	36.0	36.0	36.0	32.0	36.0
24	33.873065155279185	36.0	36.0	36.0	32.0	36.0
25	33.43844366126917	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	56.0
5	156.0
6	238.0
7	49.0
8	198.0
9	246.0
10	168.0
11	50.0
12	95.0
13	89.0
14	240.0
15	283.0
16	440.0
17	614.0
18	908.0
19	1351.0
20	2169.0
21	3162.0
22	4654.0
23	6852.0
24	9105.0
25	12632.0
26	16829.0
27	21353.0
28	28625.0
29	37810.0
30	48871.0
31	66181.0
32	95008.0
33	136368.0
34	305945.0
35	572292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73546068037715	17.535827054114787	10.813779440096436	25.914932825411626
2	17.035544219421205	20.28158899456225	36.81458185541962	25.86828493059693
3	19.519348915599817	24.096503130801743	28.2467728742996	28.13737507929883
4	12.735811260666047	15.0346660217626	35.88038874894924	36.34913396862211
5	14.757176298815553	36.41266295529672	33.15067914281489	15.679481603072846
6	33.75410687754892	35.42950160777561	16.957824763023417	13.85856675165204
7	29.803074142366754	30.495448549293414	20.88631212238264	18.815165185957188
8	28.202004827272997	32.303583131225	19.85757284830247	19.63683919319954
9	27.75101060359188	13.7766502272719	18.78129772801003	39.69104144112619
10	16.244583133721402	26.266159451834643	31.251389991767603	26.237867422676352
11	37.62955757555429	20.984571947749668	22.25123560046026	19.134634876235783
12	24.840890212605828	23.668214433392674	28.81004767277095	22.68084768123055
13	29.776116192363755	19.44047642485932	25.38207835750969	25.401329025267234
14	23.5352368618323	19.881532623374955	24.944361314560563	31.63886920023218
15	25.306495055684614	27.437851537834472	22.35689484452494	24.89875856195597
16	25.488313220421038	25.7021655740729	24.286642357640172	24.522878847865886
17	23.638930453830827	25.80176585374391	25.446713744432486	25.11258994799278
18	24.659494234902123	24.451220001327236	27.1223953870466	23.766890376724042
19	25.63426030251385	24.640052857152234	25.536976305439662	24.188710534894252
20	25.612421166383864	24.1636837073912	25.50377314877268	24.720121977452255
21	26.73375343388813	23.922322478831497	24.529210017947012	24.814714069333366
22	25.790703679228354	24.56859767438807	25.02235604240427	24.6183426039793
23	24.300656969654536	24.294822425653322	25.74798854095558	25.65653206373656
24	24.627022958772518	25.081443439564115	25.55956583919014	24.731967762473225
25	24.645014968040226	24.683009360038795	25.57248651782491	25.099489154096066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	187.0
2	560.0
3	933.0
4	933.0
5	933.0
6	2609.0
7	4285.0
8	4285.0
9	4285.0
10	4409.5
11	4534.0
12	4534.0
13	4534.0
14	4275.0
15	4016.0
16	4016.0
17	4016.0
18	6893.5
19	9771.0
20	9771.0
21	9771.0
22	15580.5
23	21390.0
24	21390.0
25	21390.0
26	32231.5
27	43073.0
28	43073.0
29	43073.0
30	54265.5
31	65458.0
32	65458.0
33	65458.0
34	77211.5
35	88965.0
36	88965.0
37	88965.0
38	98963.5
39	108962.0
40	108962.0
41	108962.0
42	123528.5
43	138095.0
44	138095.0
45	138095.0
46	157880.0
47	177665.0
48	177665.0
49	177665.0
50	177376.5
51	177088.0
52	177088.0
53	177088.0
54	161669.5
55	146251.0
56	146251.0
57	146251.0
58	135247.0
59	124243.0
60	124243.0
61	124243.0
62	112639.5
63	101036.0
64	101036.0
65	101036.0
66	85262.5
67	69489.0
68	69489.0
69	69489.0
70	53507.5
71	37526.0
72	37526.0
73	37526.0
74	30303.0
75	23080.0
76	23080.0
77	23080.0
78	18968.5
79	14857.0
80	14857.0
81	14857.0
82	10774.5
83	6692.0
84	6692.0
85	6692.0
86	5064.5
87	3437.0
88	3437.0
89	3437.0
90	2313.0
91	1189.0
92	1189.0
93	1189.0
94	706.0
95	223.0
96	223.0
97	223.0
98	410.5
99	598.0
100	598.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009322359168649489
2	0.0035687156192486325
3	0.005243827032365337
4	0.016678283200161976
5	0.03670678922655736
6	0.04734010515329819
7	0.06940787724783565
8	0.09132998748036296
9	0.09744778568478919
10	0.11886007940028098
11	0.11980688150334694
12	0.1325522944292349
13	0.1210450073304332
14	0.12344842805360064
15	0.1142717307469613
16	0.11186831002379387
17	0.12344842805360064
18	0.1292020716030015
19	0.13109567580913342
20	0.11958838871033173
21	0.12993038091305226
22	0.1492305776293969
23	0.13830593797863577
24	0.13444589863536685
25	0.13022170463707256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1373043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.03971388432428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99618960022418	37.75871108178179
2	13.486816587785583	13.227792533507273
3	4.146484341298582	6.100272171693398
4	1.697625452276539	3.3300426584955223
5	0.8821170693427964	2.1629384346524687
6	0.5804067567963606	1.7077788773893265
7	0.3751840237924971	1.28792420265275
8	0.2645624391144602	1.0379253054969673
9	0.20621864627700137	0.9101613069933141
>10	1.130728067950562	10.29904541436586
>50	0.10877498638051698	3.729901555650749
>100	0.10807106972404959	11.125139677349472
>500	0.012652933788828426	4.105495206770974
>1k	0.004168025248084658	3.2168715732001516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4495	0.3273750348678082	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2940	0.21412293715491795	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2875	0.20938892663958814	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2280	0.16605452269156903	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1828	0.1331349418772755	No Hit
GTACATGGAAGCAGTGGTATCAACG	1786	0.1300760427750624	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1582	0.11521853285002728	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1548	0.11274228119585476	No Hit
TCCATGTACTCTGCGTTGATACCAC	1546	0.11259661933384461	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1517	0.11048452233469745	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1490	0.10851808719756047	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1435	0.10451238599228138	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1416	0.10312859830318498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.283093100507413E-5	0.0
8	0.0	0.0	0.0	7.283093100507413E-5	0.0
9	0.0	0.0	0.0	2.184927930152224E-4	0.0
10	0.0	0.0	0.0	2.184927930152224E-4	1.4566186201014827E-4
11	0.0	0.0	0.0	2.184927930152224E-4	1.4566186201014827E-4
12	0.0	0.0	0.0	2.184927930152224E-4	2.9132372402029653E-4
13	0.0	0.0	0.0	2.184927930152224E-4	2.9132372402029653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	250	0.0	13.677899	5
CGCGTAT	50	0.0014978291	13.301839	7
ATAACGA	110	2.737579E-9	12.956808	12
CAAGACG	260	0.0	12.7851	4
AGTCCGG	60	4.0867133E-4	12.668418	10
TACACCG	75	1.4806179E-5	12.664722	5
TTAGAGT	165	0.0	12.663338	4
CGGTCCA	250	0.0	12.541733	10
GAACAGT	245	0.0	12.408521	6
AGGCCCG	295	0.0	12.238979	10
GGTCCAA	280	0.0	12.215974	11
GCGTTAT	140	5.2750693E-11	12.20618	1
TAATACC	55	0.0030741773	12.08773	4
GTTATTC	160	3.6379788E-12	11.871013	3
CGCCAGT	265	0.0	11.833118	18
CTATAGC	65	8.04008E-4	11.689234	4
GAGTACG	65	8.0671697E-4	11.684548	1
ATATACG	205	0.0	11.585699	6
TCGCCAG	280	0.0	11.538151	17
ATCGCCA	265	0.0	11.473702	16
>>END_MODULE