FastQCFastQC Report
Thu 2 Feb 2017
SRR4062495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373043
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15370.11194114095479893No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14890.10844525626655538No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14630.10655165206042345No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14220.10356558388921543No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14090.10261878178614944No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13760.10021536106298201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACG654.9304566E-420.3090212
TAGGACG6000.020.1665174
GATATAC4650.019.8751641
GTAGGAC7950.019.0948933
GTCCTAA4500.019.0706921
TGTAGGA7550.018.9416372
TCCTACA7900.018.102452
TAGGACC8050.018.0371344
ATACACT5350.017.682114
TTTACGG750.001290740917.600513
CGAATGC5150.017.51443343
ATACGAA4900.017.51007540
ATAATAC1408.371717E-817.2862153
CGCAATA5100.017.25477236
AATCCCG3100.017.0321319
CGTCTTA1954.9112714E-1116.9229515
AGGACGT6250.016.8958745
CTACGAC4750.016.673564
GACGGTA4500.016.6220998
CGACGGT4800.016.4998767
Produced by FastQC (version 0.11.5)