##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1373043
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23381933413593	32.0	32.0	32.0	32.0	32.0
2	31.38941970499103	32.0	32.0	32.0	32.0	32.0
3	31.478283637147563	32.0	32.0	32.0	32.0	32.0
4	31.58248357844583	32.0	32.0	32.0	32.0	32.0
5	31.51428105310613	32.0	32.0	32.0	32.0	32.0
6	35.09455057124941	36.0	36.0	36.0	36.0	36.0
7	35.10067710916555	36.0	36.0	36.0	36.0	36.0
8	35.043526677605875	36.0	36.0	36.0	36.0	36.0
9	35.16700642295981	36.0	36.0	36.0	36.0	36.0
10	35.03646499053562	36.0	36.0	36.0	36.0	36.0
11	35.17202811565261	36.0	36.0	36.0	36.0	36.0
12	35.076869406129305	36.0	36.0	36.0	36.0	36.0
13	35.12264364626599	36.0	36.0	36.0	36.0	36.0
14	35.085074538816336	36.0	36.0	36.0	36.0	36.0
15	35.04977629979542	36.0	36.0	36.0	36.0	36.0
16	35.06433374628471	36.0	36.0	36.0	36.0	36.0
17	35.03038506441532	36.0	36.0	36.0	36.0	36.0
18	35.055080576500515	36.0	36.0	36.0	36.0	36.0
19	35.024182054021615	36.0	36.0	36.0	36.0	36.0
20	35.005871629657626	36.0	36.0	36.0	36.0	36.0
21	34.991569091426854	36.0	36.0	36.0	36.0	36.0
22	34.98616867789283	36.0	36.0	36.0	36.0	36.0
23	34.94934608748597	36.0	36.0	36.0	32.0	36.0
24	34.93063217976422	36.0	36.0	36.0	32.0	36.0
25	34.90676548367385	36.0	36.0	36.0	32.0	36.0
26	34.858817968555975	36.0	36.0	36.0	32.0	36.0
27	34.84533914815486	36.0	36.0	36.0	32.0	36.0
28	34.82464933727494	36.0	36.0	36.0	32.0	36.0
29	34.79515790838306	36.0	36.0	36.0	32.0	36.0
30	34.78597829783918	36.0	36.0	36.0	32.0	36.0
31	34.783625130458404	36.0	36.0	36.0	32.0	36.0
32	34.7380162165351	36.0	36.0	36.0	32.0	36.0
33	34.71201703078491	36.0	36.0	36.0	32.0	36.0
34	34.69229295805011	36.0	36.0	36.0	32.0	36.0
35	34.65717388311946	36.0	36.0	36.0	32.0	36.0
36	34.62905677389565	36.0	36.0	36.0	32.0	36.0
37	34.61488897288723	36.0	36.0	36.0	32.0	36.0
38	34.57164123774711	36.0	36.0	36.0	32.0	36.0
39	34.54800905725458	36.0	36.0	36.0	32.0	36.0
40	34.54316798527067	36.0	36.0	36.0	32.0	36.0
41	34.50580935921162	36.0	36.0	36.0	32.0	36.0
42	34.46664525437295	36.0	36.0	36.0	32.0	36.0
43	34.459228880668704	36.0	36.0	36.0	32.0	36.0
44	34.410332378519826	36.0	36.0	36.0	32.0	36.0
45	34.3563093071375	36.0	36.0	36.0	32.0	36.0
46	34.34049261385113	36.0	36.0	36.0	32.0	36.0
47	34.312073984572955	36.0	36.0	36.0	32.0	36.0
48	34.264492809038025	36.0	36.0	36.0	32.0	36.0
49	34.241704739035846	36.0	36.0	36.0	32.0	36.0
50	33.711883021871856	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	39.0
22	120.0
23	419.0
24	954.0
25	2314.0
26	4686.0
27	8756.0
28	15298.0
29	23145.0
30	33623.0
31	47800.0
32	69912.0
33	113118.0
34	241179.0
35	811673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35541498613447	18.20335963182596	11.26491354713515	26.17631183490442
2	16.50162492552728	20.847772664834164	36.38085166199801	26.269750747640547
3	18.938801907892962	24.374796527104593	28.57294762762439	28.113453937378054
4	12.499763299301843	15.539510080536504	35.99620404911139	35.96452257105027
5	14.497652294939051	36.737014062924466	32.92839335694512	15.836940285191359
6	34.0364710029453	35.66170151401711	16.60517277041534	13.696654712622248
7	29.912100349369975	30.49613158509967	20.79075455029449	18.80101351523587
8	28.320307521323073	32.496141781430005	19.673819392400674	19.50973130484624
9	27.917261149140998	13.560172551041738	18.63714392047445	39.88542237934281
10	16.20510064142201	26.36530684035387	31.203756910744968	26.225835607479155
11	37.967274149462185	20.774149098025337	22.133319932442028	19.125256820070458
12	24.906357630460228	23.598459771471106	28.698591376963428	22.79659122110524
13	29.979833115204695	19.248705248124057	25.413188079324538	25.35827355734671
14	23.696927190189964	19.742717453131476	24.89623413105052	31.66412122562804
15	25.55229515754423	27.34116848489086	22.23841496588235	24.868121391682564
16	25.802105250891632	25.552222326613222	24.101284519130136	24.54438790336501
17	23.89204125435256	25.7743566661787	25.253615509492423	25.07998656997632
18	24.90970785328646	24.46289009157033	26.980946700139764	23.646455355003447
19	25.810699300750233	24.64875462749528	25.344508511386753	24.19603756036774
20	25.799410506444442	24.0648690536276	25.346839101178915	24.788881338749043
21	26.968492610937894	23.885195146838083	24.334489160208385	24.811823082015643
22	25.927447283151366	24.508190930655484	24.951148653028348	24.613213133164802
23	24.447741257921273	24.21774117780725	25.615949391242665	25.71856817302881
24	24.861275284168087	25.004606556386072	25.374514854960843	24.759603304485
25	24.88144945205649	24.507462621345436	25.488568092914786	25.12251983368329
26	24.13209200294528	25.403938551086892	26.07500274936764	24.38896669660018
27	25.257694041628703	24.837313907867415	25.255946099284582	24.649045951219296
28	24.25838083730808	24.731490565117042	25.81273856681837	25.1973900307565
29	24.33485331486341	24.760331613795053	25.587545328150686	25.317269743190856
30	24.133330128772368	25.169714276974574	25.9451451993856	24.75181039486746
31	24.960762335921018	24.805486791018197	24.849622335207272	25.384128537853513
32	24.454004717987708	25.1701512625606	24.93942287313653	25.43642114631516
33	24.298583511222883	24.51023019672363	25.72366633819917	25.46751995385432
34	24.914951680318826	24.724644457602565	25.982288974198188	24.37811488788042
35	25.543701107685628	24.702649516439035	25.59031290352888	24.163336472346458
36	24.241047075728876	25.350917633315202	25.21909364819601	25.188941642759914
37	25.252158890872316	25.01808027862201	24.928425402554762	24.80133542795091
38	24.52749112737183	24.931265808863962	25.402263439673778	25.138979624090435
39	25.082462821630497	24.336528426276526	25.219821957506067	25.361186794586914
40	25.45899873492673	24.639796422981654	25.633865800269913	24.267339041821707
41	24.216138897325138	24.90941652956244	26.353144074876024	24.5213004982364
42	25.426661801560474	25.462640281476983	25.507504134976106	23.603193781986434
43	24.36871969778077	24.28219655174674	25.917178121879648	25.431905628592844
44	23.84295320685514	25.06017655674294	25.62133888013704	25.47553135626488
45	24.07433707465826	25.201177239168764	25.606918355798037	25.11756733037494
46	23.931640738798578	24.634679251879042	25.76712987239993	25.66655013692245
47	24.38954934404822	24.51154115348172	26.052789315411097	25.04612018705896
48	24.713428494227784	25.386604789507683	25.034612899960162	24.86535381630437
49	23.90150927538322	25.240505941911508	25.616896193345728	25.241088589359546
50	23.75930507537648	25.93440399813261	25.046884980128052	25.259405946362858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	22.5
2	26.0
3	93.5
4	161.0
5	181.0
6	201.0
7	207.5
8	214.0
9	324.0
10	434.0
11	616.5
12	799.0
13	1363.0
14	1927.0
15	2842.0
16	3757.0
17	4533.0
18	5309.0
19	5884.0
20	6459.0
21	7285.0
22	8111.0
23	8527.5
24	8944.0
25	9709.5
26	10475.0
27	12780.0
28	15085.0
29	17325.5
30	19566.0
31	22182.5
32	24799.0
33	29069.0
34	33339.0
35	38412.5
36	43486.0
37	49618.5
38	55751.0
39	58383.0
40	61015.0
41	64721.0
42	68427.0
43	69387.5
44	70348.0
45	77008.0
46	83668.0
47	89332.5
48	94997.0
49	97693.0
50	100389.0
51	95942.0
52	91495.0
53	89326.5
54	87158.0
55	89120.0
56	91082.0
57	89008.0
58	86934.0
59	79945.5
60	72957.0
61	65043.5
62	57130.0
63	50388.0
64	43646.0
65	38619.0
66	33592.0
67	29656.5
68	25721.0
69	23782.0
70	21843.0
71	17595.0
72	13347.0
73	12271.0
74	11195.0
75	8364.0
76	5533.0
77	4995.5
78	4458.0
79	3978.5
80	3499.0
81	2834.0
82	2169.0
83	1953.0
84	1737.0
85	1396.5
86	1056.0
87	798.5
88	541.0
89	355.0
90	169.0
91	107.5
92	46.0
93	28.5
94	11.0
95	10.0
96	9.0
97	7.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015585819235085865
2	0.004151363067289225
3	7.283093100507413E-4
4	7.283093100507413E-5
5	0.0
6	5.09816517035519E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.184927930152224E-4
47	0.0
48	0.0
49	0.0
50	1.4566186201014827E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1373043.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.87263716888048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14226693267243	42.09729994017668
2	13.215139195984335	14.238687980830298
3	3.875282161039207	6.263150093661007
4	1.5773101397773739	3.3989542745209045
5	0.809038979127425	2.179253168900661
6	0.495459151836452	1.6015014671331862
7	0.3208638876123849	1.2100048658556914
8	0.24115664083504507	1.0393395370057927
9	0.18244464118834738	0.8845896562331782
>10	0.9573396345842901	9.420061059880867
>50	0.08696883847382991	3.259212480341041
>100	0.08587655097198835	9.784997355837325
>500	0.008818262291243188	3.24234182459097
>1k	0.002034983605671505	1.380606295032424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1537	0.11194114095479893	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1489	0.10844525626655538	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1463	0.10655165206042345	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1422	0.10356558388921543	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1409	0.10261878178614944	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1376	0.10021536106298201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.9132372402029653E-4
13	0.0	0.0	0.0	0.0	2.9132372402029653E-4
14	0.0	0.0	0.0	0.0	2.9132372402029653E-4
15	0.0	0.0	0.0	0.0	2.9132372402029653E-4
16	0.0	0.0	0.0	0.0	2.9132372402029653E-4
17	0.0	0.0	0.0	7.283093100507413E-5	2.9132372402029653E-4
18	0.0	0.0	0.0	2.9132372402029653E-4	2.9132372402029653E-4
19	0.0	0.0	0.0	3.6415465502537064E-4	2.9132372402029653E-4
20	0.0	0.0	0.0	5.826474480405931E-4	5.09816517035519E-4
21	0.0	0.0	0.0	7.283093100507413E-4	5.09816517035519E-4
22	0.0	0.0	0.0	0.0013837876890964084	5.09816517035519E-4
23	0.0	0.0	0.0	0.002184927930152224	5.09816517035519E-4
24	0.0	0.0	0.0	0.0039328702742740035	5.09816517035519E-4
25	0.0	0.0	0.0	0.005243827032365337	5.09816517035519E-4
26	0.0	0.0	0.0	0.0068461075144769686	5.09816517035519E-4
27	0.0	0.0	0.0	0.009613682892669785	5.09816517035519E-4
28	0.0	0.0	0.0	0.017770747165238087	5.09816517035519E-4
29	0.0	0.0	0.0	0.03685245108856751	5.09816517035519E-4
30	0.0	0.0	0.0	0.07924005293352065	5.09816517035519E-4
31	0.0	0.0	0.0	0.15971823169412758	5.09816517035519E-4
32	0.0	0.0	0.0	0.25155803569152607	5.09816517035519E-4
33	0.0	0.0	0.0	0.34609258413611227	5.09816517035519E-4
34	0.0	0.0	0.0	0.45504765691970317	5.09816517035519E-4
35	0.0	0.0	0.0	0.5752915240090806	5.09816517035519E-4
36	0.0	0.0	0.0	0.74666270466402	5.09816517035519E-4
37	0.0	0.0	0.0	0.9877330862908154	5.09816517035519E-4
38	0.0	0.0	0.0	1.297774359579416	5.09816517035519E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTACG	65	4.9304566E-4	20.309021	2
TAGGACG	600	0.0	20.166517	4
GATATAC	465	0.0	19.875164	1
GTAGGAC	795	0.0	19.094893	3
GTCCTAA	450	0.0	19.070692	1
TGTAGGA	755	0.0	18.941637	2
TCCTACA	790	0.0	18.10245	2
TAGGACC	805	0.0	18.037134	4
ATACACT	535	0.0	17.68211	4
TTTACGG	75	0.0012907409	17.60051	3
CGAATGC	515	0.0	17.514433	43
ATACGAA	490	0.0	17.510075	40
ATAATAC	140	8.371717E-8	17.286215	3
CGCAATA	510	0.0	17.254772	36
AATCCCG	310	0.0	17.03213	19
CGTCTTA	195	4.9112714E-11	16.92295	15
AGGACGT	625	0.0	16.895874	5
CTACGAC	475	0.0	16.67356	4
GACGGTA	450	0.0	16.622099	8
CGACGGT	480	0.0	16.499876	7
>>END_MODULE