FastQCFastQC Report
Thu 2 Feb 2017
SRR4062494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756605
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17480.2310320444617733No Hit
GTACATGGAAGCAGTGGTATCAACG17250.2279921491399079No Hit
TCCATGTACTCTGCGTTGATACCAC13790.18226154995010607No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12240.16177529886796943No Hit
TATCAACGCAGAGTACTTTTTTTTT11030.1457828060877208No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9430.12463570819648297No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8830.11670554648726879No Hit
GCGCAAGACGGACCAGAGCGAAAGC8270.10930406222533554No Hit
GAATAGGACCGCGGTTCTATTTTGT8030.10613199754164987No Hit
GAGTACATGGAAGCAGTGGTATCAA7970.10533898137072846No Hit
GGGTAGGCACACGCTGAGCCAGTCA7830.10348861030524514No Hit
GAATAACGCCGCCGCATCGCCAGTC7800.10309210221978442No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7640.10097739243066066No Hit
GTATCTGATCGTCTTCGAACCTCCG7590.10031654562155946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTG1150.015.6933015
CAACGGA508.695958E-515.20174914
CGAACGA653.3602555E-614.61803316
CCTATGC653.385745E-614.6073943
AATCGTA400.00527175514.25163913
AACGGAC602.5620351E-514.25163715
GTATTAA801.2961391E-714.2403251
GTCTAAA400.005299607314.2403251
GGTTCGT759.626874E-713.93678118
AACCGCG551.9561444E-413.8197717
CGACTGG551.9561444E-413.81977115
ATAACGA707.23222E-613.57478712
TAACGAA707.242121E-613.572988513
CCGTCAA1001.0004442E-813.30329118
TTAGAGT1153.765308E-1013.2145374
GTATTAC655.4800694E-513.1449161
TTTTCGG801.9914678E-613.06486716
TTAACTG801.9988838E-613.0605445
CGGTCGG905.385118E-712.66812410
TCCGATA751.4744581E-512.6681238
Produced by FastQC (version 0.11.5)