##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062494_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756605
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01688992274701	32.0	32.0	32.0	32.0	32.0
2	30.538759326200594	32.0	32.0	32.0	32.0	32.0
3	30.528109119025117	32.0	32.0	32.0	32.0	32.0
4	30.613021325526528	32.0	32.0	32.0	32.0	32.0
5	30.31457365468111	32.0	32.0	32.0	27.0	32.0
6	33.958184257307316	36.0	36.0	36.0	32.0	36.0
7	33.818376828067485	36.0	36.0	36.0	32.0	36.0
8	33.779065694781295	36.0	36.0	36.0	32.0	36.0
9	34.032996081178425	36.0	36.0	36.0	32.0	36.0
10	33.55428922621447	36.0	36.0	36.0	27.0	36.0
11	34.03241850106727	36.0	36.0	36.0	32.0	36.0
12	33.71705579529609	36.0	36.0	36.0	32.0	36.0
13	33.90804977498166	36.0	36.0	36.0	32.0	36.0
14	33.77197745190687	36.0	36.0	36.0	32.0	36.0
15	33.685673502025494	36.0	36.0	36.0	27.0	36.0
16	33.681688595766616	36.0	36.0	36.0	27.0	36.0
17	33.58941984258629	36.0	36.0	36.0	27.0	36.0
18	33.619767249753835	36.0	36.0	36.0	27.0	36.0
19	33.59637459440527	36.0	36.0	36.0	27.0	36.0
20	33.58939737379478	36.0	36.0	36.0	27.0	36.0
21	33.57780876415038	36.0	36.0	36.0	27.0	36.0
22	33.50582007784775	36.0	36.0	36.0	27.0	36.0
23	33.46647458052749	36.0	36.0	36.0	27.0	36.0
24	33.44693069699513	36.0	36.0	36.0	27.0	36.0
25	32.93621242259832	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	37.0
5	65.0
6	129.0
7	45.0
8	104.0
9	127.0
10	83.0
11	31.0
12	62.0
13	63.0
14	114.0
15	169.0
16	254.0
17	375.0
18	523.0
19	772.0
20	1360.0
21	1979.0
22	3121.0
23	4458.0
24	6384.0
25	8993.0
26	12189.0
27	15222.0
28	20286.0
29	26341.0
30	33153.0
31	44285.0
32	61800.0
33	84323.0
34	169035.0
35	260717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.790404788089	16.845153751797646	10.514127400389137	25.850314059724223
2	17.054066842721607	19.887864104617872	36.46531373995152	26.592755312709
3	19.476008390421594	23.930941596162718	27.836426431149768	28.756623582265917
4	12.571761685770069	14.75551125781067	35.571324142726276	37.10140291369298
5	14.6676221288208	36.563747350774044	33.4363716412839	15.332258879121257
6	34.77982040781785	34.8931398748329	16.596475592679326	13.730564124669925
7	30.4820904030271	30.25103722640963	20.706834915348836	18.560037455214434
8	28.63520855298057	32.307688237402886	19.59251111519723	19.464592094419316
9	27.339837936166695	13.958127997354062	18.312948569538616	40.38908549694063
10	15.896104446050058	26.409521465378294	31.664509674704437	26.02986441386721
11	37.793292725691984	20.9296970835809	21.771183042714775	19.50582714801234
12	24.453085089769484	23.637576396039183	28.90278215250886	23.006556361682474
13	29.570900583433353	19.235700986768542	25.323706925641027	25.86969150415708
14	23.426394360493813	19.56147466681841	24.984880946551996	32.02725002613578
15	25.412836024706646	26.84677961436881	22.572074289313367	25.16831007161117
16	26.086651617837365	25.63426052116936	23.6600940507726	24.618993810220676
17	23.982850110495043	25.703793883735393	25.340020378726724	24.973335627042836
18	25.04787427626137	24.642779156327542	26.334888337468982	23.9744582299421
19	25.37287359485878	25.35858065848087	25.263029731954507	24.005516014705844
20	25.24269146689392	24.276689444906125	25.494248979743073	24.986370108456885
21	26.586558503000745	24.18569566396919	24.31909163694592	24.90865419608414
22	25.395281114530594	24.49650881895496	25.031933551738973	25.076276514775476
23	24.370471937593805	24.185445083553255	25.332664519049295	26.11141845980364
24	24.36177393099615	25.442490173502204	25.091118434112836	25.104617461388813
25	24.926815453188357	24.628386604978136	25.117121743089154	25.32767619874435
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	134.0
2	288.5
3	443.0
4	443.0
5	443.0
6	1280.0
7	2117.0
8	2117.0
9	2117.0
10	2172.5
11	2228.0
12	2228.0
13	2228.0
14	2290.5
15	2353.0
16	2353.0
17	2353.0
18	3670.5
19	4988.0
20	4988.0
21	4988.0
22	8073.5
23	11159.0
24	11159.0
25	11159.0
26	16482.0
27	21805.0
28	21805.0
29	21805.0
30	27049.5
31	32294.0
32	32294.0
33	32294.0
34	39096.0
35	45898.0
36	45898.0
37	45898.0
38	51689.0
39	57480.0
40	57480.0
41	57480.0
42	66446.5
43	75413.0
44	75413.0
45	75413.0
46	87541.5
47	99670.0
48	99670.0
49	99670.0
50	100056.0
51	100442.0
52	100442.0
53	100442.0
54	93438.5
55	86435.0
56	86435.0
57	86435.0
58	79814.0
59	73193.0
60	73193.0
61	73193.0
62	65531.5
63	57870.0
64	57870.0
65	57870.0
66	48360.0
67	38850.0
68	38850.0
69	38850.0
70	29547.5
71	20245.0
72	20245.0
73	20245.0
74	15848.0
75	11451.0
76	11451.0
77	11451.0
78	9281.5
79	7112.0
80	7112.0
81	7112.0
82	4946.0
83	2780.0
84	2780.0
85	2780.0
86	2054.5
87	1329.0
88	1329.0
89	1329.0
90	887.5
91	446.0
92	446.0
93	446.0
94	274.0
95	102.0
96	102.0
97	102.0
98	235.0
99	368.0
100	368.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008062331071034424
2	0.0030398953218654383
3	0.0037007421309666203
4	0.014274291076585538
5	0.03330667917869959
6	0.04440890557159945
7	0.06634901963375871
8	0.08696744007771559
9	0.09648363412877262
10	0.12199232096007824
11	0.12133147415097706
12	0.13256586990569716
13	0.11974544180913423
14	0.12371052266374132
15	0.11326914307994264
16	0.11141877201445932
17	0.12093496606551636
18	0.12952597458383172
19	0.13045116011657337
20	0.12067062734187589
21	0.126882587347427
22	0.1493513788568672
23	0.13732396693122567
24	0.13150851501113528
25	0.12965814394565195
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	756605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.67917739061071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73317975474478	45.75890216627241
2	11.972746493340274	13.572108446976916
3	3.369929240674552	5.73014451677997
4	1.3392005601288897	3.036191444366023
5	0.6734686927464082	1.9085825751598187
6	0.379722214683315	1.2913405659114705
7	0.25703148885709565	1.0197833346332876
8	0.16902969132728587	0.7664371087215528
9	0.13822239276084536	0.705089836678196
>10	0.7657643680968883	8.32272746486182
>50	0.09676257672696645	3.876847648682676
>100	0.09722960084603219	10.558793024948043
>500	0.006544300056593181	2.5020252663734204
>1k	0.0011686250101059252	0.9510265996343932
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1748	0.2310320444617733	No Hit
GTACATGGAAGCAGTGGTATCAACG	1725	0.2279921491399079	No Hit
TCCATGTACTCTGCGTTGATACCAC	1379	0.18226154995010607	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1224	0.16177529886796943	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1103	0.1457828060877208	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	943	0.12463570819648297	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	883	0.11670554648726879	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	827	0.10930406222533554	No Hit
GAATAGGACCGCGGTTCTATTTTGT	803	0.10613199754164987	No Hit
GAGTACATGGAAGCAGTGGTATCAA	797	0.10533898137072846	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	783	0.10348861030524514	No Hit
GAATAACGCCGCCGCATCGCCAGTC	780	0.10309210221978442	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	764	0.10097739243066066	No Hit
GTATCTGATCGTCTTCGAACCTCCG	759	0.10031654562155946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3216936182023644E-4
2	0.0	0.0	0.0	0.0	1.3216936182023644E-4
3	0.0	0.0	0.0	0.0	1.3216936182023644E-4
4	0.0	0.0	0.0	0.0	1.3216936182023644E-4
5	0.0	0.0	0.0	0.0	1.3216936182023644E-4
6	0.0	0.0	0.0	0.0	1.3216936182023644E-4
7	0.0	0.0	0.0	1.3216936182023644E-4	1.3216936182023644E-4
8	0.0	0.0	0.0	1.3216936182023644E-4	1.3216936182023644E-4
9	0.0	0.0	0.0	2.643387236404729E-4	1.3216936182023644E-4
10	0.0	0.0	0.0	2.643387236404729E-4	1.3216936182023644E-4
11	0.0	0.0	0.0	2.643387236404729E-4	1.3216936182023644E-4
12	0.0	0.0	0.0	3.9650808546070937E-4	2.643387236404729E-4
13	0.0	0.0	0.0	3.9650808546070937E-4	2.643387236404729E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTG	115	0.0	15.693301	5
CAACGGA	50	8.695958E-5	15.201749	14
CGAACGA	65	3.3602555E-6	14.618033	16
CCTATGC	65	3.385745E-6	14.607394	3
AATCGTA	40	0.005271755	14.251639	13
AACGGAC	60	2.5620351E-5	14.251637	15
GTATTAA	80	1.2961391E-7	14.240325	1
GTCTAAA	40	0.0052996073	14.240325	1
GGTTCGT	75	9.626874E-7	13.936781	18
AACCGCG	55	1.9561444E-4	13.819771	7
CGACTGG	55	1.9561444E-4	13.819771	15
ATAACGA	70	7.23222E-6	13.574787	12
TAACGAA	70	7.242121E-6	13.5729885	13
CCGTCAA	100	1.0004442E-8	13.303291	18
TTAGAGT	115	3.765308E-10	13.214537	4
GTATTAC	65	5.4800694E-5	13.144916	1
TTTTCGG	80	1.9914678E-6	13.064867	16
TTAACTG	80	1.9988838E-6	13.060544	5
CGGTCGG	90	5.385118E-7	12.668124	10
TCCGATA	75	1.4744581E-5	12.668123	8
>>END_MODULE