FastQCFastQC Report
Thu 2 Feb 2017
SRR4062494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756605
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8940.11815940946729138No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7990.10560332009436893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7840.10362077966706539No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7830.10348861030524514No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG7730.10216691668704278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT609.747253E-625.6664756
CCGGTAG551.5929315E-423.999824
CGTCGTA1950.021.43573610
GTATAGA1451.2732926E-1121.245431
CCGTCGT1900.020.8419489
TTAACGG755.469483E-520.53317835
ACCGTCG2100.019.9046128
ATACCGT2000.019.799856
CTAGCGG2450.019.75495329
TACCGTC1950.019.1793427
AATACGA2450.018.85700239
GTAAACG1759.094947E-1218.85727
TAGAACA2250.018.577644
TCTAGCG2500.018.4798628
CGCAATA2500.018.4798636
GTATAGT600.007401454718.3368321
CGGGATT600.007409924618.33319744
CGGTCCA2650.018.26401510
CCCGTTT1102.7938222E-617.99986522
GCAATAC2700.017.9257937
Produced by FastQC (version 0.11.5)