##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756605
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20854474924168	32.0	32.0	32.0	32.0	32.0
2	31.33422723878378	32.0	32.0	32.0	32.0	32.0
3	31.455864024160558	32.0	32.0	32.0	32.0	32.0
4	31.573270068265476	32.0	32.0	32.0	32.0	32.0
5	31.504709855208464	32.0	32.0	32.0	32.0	32.0
6	35.08191196198809	36.0	36.0	36.0	36.0	36.0
7	35.0900086570932	36.0	36.0	36.0	36.0	36.0
8	35.02953720897958	36.0	36.0	36.0	36.0	36.0
9	35.17067294030571	36.0	36.0	36.0	36.0	36.0
10	35.03991646896333	36.0	36.0	36.0	36.0	36.0
11	35.176969488702824	36.0	36.0	36.0	36.0	36.0
12	35.07904653022383	36.0	36.0	36.0	36.0	36.0
13	35.12757118972251	36.0	36.0	36.0	36.0	36.0
14	35.09129598667733	36.0	36.0	36.0	36.0	36.0
15	35.04725186854435	36.0	36.0	36.0	36.0	36.0
16	35.061482543731536	36.0	36.0	36.0	36.0	36.0
17	35.03220042162027	36.0	36.0	36.0	36.0	36.0
18	35.03547293501893	36.0	36.0	36.0	36.0	36.0
19	35.0228362223353	36.0	36.0	36.0	36.0	36.0
20	35.01868478268052	36.0	36.0	36.0	36.0	36.0
21	35.010518037813654	36.0	36.0	36.0	36.0	36.0
22	34.98903390804978	36.0	36.0	36.0	36.0	36.0
23	34.95324905333695	36.0	36.0	36.0	32.0	36.0
24	34.92706498106674	36.0	36.0	36.0	32.0	36.0
25	34.90417853437395	36.0	36.0	36.0	32.0	36.0
26	34.8496150567337	36.0	36.0	36.0	32.0	36.0
27	34.8333793723277	36.0	36.0	36.0	32.0	36.0
28	34.82003026678386	36.0	36.0	36.0	32.0	36.0
29	34.793600359500665	36.0	36.0	36.0	32.0	36.0
30	34.77509797053945	36.0	36.0	36.0	32.0	36.0
31	34.77462612591775	36.0	36.0	36.0	32.0	36.0
32	34.72693413339854	36.0	36.0	36.0	32.0	36.0
33	34.700808215647534	36.0	36.0	36.0	32.0	36.0
34	34.68545013580402	36.0	36.0	36.0	32.0	36.0
35	34.64427144943531	36.0	36.0	36.0	32.0	36.0
36	34.619639045472866	36.0	36.0	36.0	32.0	36.0
37	34.59041904296165	36.0	36.0	36.0	32.0	36.0
38	34.558796201452544	36.0	36.0	36.0	32.0	36.0
39	34.53949154446508	36.0	36.0	36.0	32.0	36.0
40	34.52153765835541	36.0	36.0	36.0	32.0	36.0
41	34.48898170115186	36.0	36.0	36.0	32.0	36.0
42	34.43200613265839	36.0	36.0	36.0	32.0	36.0
43	34.44274224991904	36.0	36.0	36.0	32.0	36.0
44	34.39865583759029	36.0	36.0	36.0	32.0	36.0
45	34.336101400334385	36.0	36.0	36.0	32.0	36.0
46	34.35049464383661	36.0	36.0	36.0	32.0	36.0
47	34.315715597967234	36.0	36.0	36.0	32.0	36.0
48	34.27770765458859	36.0	36.0	36.0	32.0	36.0
49	34.25465203111267	36.0	36.0	36.0	32.0	36.0
50	33.72479827651152	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	19.0
22	60.0
23	180.0
24	551.0
25	1268.0
26	2677.0
27	5019.0
28	8417.0
29	12640.0
30	18151.0
31	26047.0
32	39035.0
33	63117.0
34	136014.0
35	443400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16665036338689	17.496503607431123	11.081647706392912	26.255198322789074
2	16.435556930185957	20.479613834597597	36.11035109910953	26.974478136106917
3	18.954111876669476	24.133327870304164	28.259544991349472	28.653015261676895
4	12.225533799010051	15.256441604271714	35.78379735793445	36.73422723878378
5	14.350024120908532	36.6697285902155	33.350427237462085	15.629820051413882
6	34.979421148767784	35.09612715800381	16.315447223242867	13.609004469985543
7	30.42525492165661	30.28991349515269	20.67974702784148	18.605084555349226
8	28.52518817612889	32.45471547240634	19.45308318078786	19.567013170676905
9	27.34584096060692	13.766628557834009	18.214391921808605	40.67313855975046
10	15.686388538272944	26.389595627837508	31.725140595158635	26.198875238730913
11	38.08988838297394	20.845883915649512	21.647491095089247	19.416736606287298
12	24.474990252509564	23.714223405872286	28.6421580613398	23.16862828027835
13	29.697926923559848	19.181342972885457	25.361846670323352	25.758883433231343
14	23.4674632073539	19.523000773190766	24.91471771928549	32.094818300169834
15	25.436786698475427	26.87637538742144	22.377594649784232	25.309243264318898
16	26.072256990107125	25.642045717382256	23.573595204895554	24.71210208761507
17	24.03658447935184	25.598826336067038	25.345193330734002	25.019395853847122
18	25.272764520456516	24.48820718869159	26.24288763621705	23.99614065463485
19	25.553888753048152	25.211966614019204	25.131739811394322	24.10240482153832
20	25.255714672781703	24.240389635278646	25.51185889598932	24.99203679595033
21	26.726098823031837	24.20391089141626	24.29920500128865	24.770785284263255
22	25.418679495906055	24.439965371627203	25.095525406255575	25.045829726211167
23	24.43956886354174	24.07491359427971	25.418150818458773	26.067366723719775
24	24.483581261027883	25.232320695739517	25.307260723891595	24.976837319341
25	25.116672504146813	24.306077808103304	25.276333093225663	25.300916594524224
26	24.53750636065054	25.123941819046923	25.778576668142556	24.55997515215998
27	25.3889413894965	24.8736130477594	25.072527937298855	24.664917625445245
28	24.58416214537308	24.357095181765914	25.849419446078205	25.2093232267828
29	24.428863145234303	24.865682886050188	25.7364146417219	24.96903932699361
30	24.603723210922475	24.96269519762624	25.925548998486658	24.508032592964625
31	24.836737795811555	24.803034608547392	24.90269030735985	25.457537288281202
32	24.619583534340904	24.901104275018007	24.962563028264416	25.516749162376666
33	24.24831979698786	24.51107248828649	25.47921306361972	25.761394651105928
34	24.81175778642753	24.732984846782667	25.951322024041605	24.5039353427482
35	25.699010712326775	24.39965371627203	25.464145756372215	24.43718981502898
36	24.523232069573954	25.247520172348846	25.126188698197872	25.103059059879328
37	25.638873652698567	24.78096232512341	25.104645092221173	24.475518929956845
38	24.57279558025654	24.658705665439694	25.37664963884722	25.391849115456544
39	25.00908664362514	24.558653458541777	25.08019376028443	25.352066137548658
40	25.469829038930484	24.703643248458576	25.381936413320027	24.44459129929091
41	24.096060692170948	25.183946709313314	25.904269731233605	24.815722867282137
42	25.688172824657517	25.1655751680203	25.265891713641857	23.880360293680322
43	24.66571064161617	24.212237561210937	25.499434975978218	25.622616821194676
44	24.37031211794794	24.85695970817005	25.45383654615024	25.318891627731777
45	24.431374363108887	25.07556783262072	25.298537546011456	25.194520258258933
46	24.329403927819477	24.872357101412497	25.38107831980576	25.417160650962263
47	24.6404662935085	24.495212164868065	25.878893213764115	24.985428327859317
48	25.34334295966852	25.48978661256534	24.50076327806451	24.666107149701627
49	24.17364410755943	25.32047766007362	25.51741000918577	24.988468223181183
50	24.184481995228683	25.68420774380291	24.749109508924736	25.382200752043673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	23.5
2	23.0
3	95.5
4	168.0
5	176.5
6	185.0
7	165.0
8	145.0
9	196.0
10	247.0
11	344.0
12	441.0
13	762.0
14	1083.0
15	1478.0
16	1873.0
17	2226.0
18	2579.0
19	2781.5
20	2984.0
21	3534.5
22	4085.0
23	4242.5
24	4400.0
25	4978.5
26	5557.0
27	7007.5
28	8458.0
29	9356.0
30	10254.0
31	10894.0
32	11534.0
33	13394.0
34	15254.0
35	17420.5
36	19587.0
37	23565.5
38	27544.0
39	29626.0
40	31708.0
41	34858.0
42	38008.0
43	38900.0
44	39792.0
45	44334.0
46	48876.0
47	52245.5
48	55615.0
49	56321.0
50	57027.0
51	54608.5
52	52190.0
53	51798.0
54	51406.0
55	52287.5
56	53169.0
57	52037.5
58	50906.0
59	46723.5
60	42541.0
61	37770.5
62	33000.0
63	28734.5
64	24469.0
65	21364.0
66	18259.0
67	15771.0
68	13283.0
69	12189.0
70	11095.0
71	8595.5
72	6096.0
73	5558.0
74	5020.0
75	3672.5
76	2325.0
77	2165.0
78	2005.0
79	1691.5
80	1378.0
81	1100.5
82	823.0
83	684.5
84	546.0
85	457.0
86	368.0
87	280.0
88	192.0
89	121.0
90	50.0
91	33.0
92	16.0
93	11.5
94	7.0
95	6.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014670799162046246
2	0.005022435749168985
3	5.286774472809459E-4
4	0.0
5	0.0
6	3.9650808546070937E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.6433872364047293E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	756605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.233139727037646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6443685926668	47.76819153048158
2	12.247917896429978	14.509652642490437
3	3.3745631129194127	5.99657905155788
4	1.2718104934507823	3.0133331465953157
5	0.652538187179835	1.9325942809225505
6	0.3957320477421385	1.4064271007026092
7	0.2424646983241272	1.005336174829494
8	0.17137904298002232	0.8121055039297321
9	0.12418072009150523	0.6620052550125852
>10	0.7021717194335373	7.91194313102872
>50	0.08666099236774033	3.699878670635828
>100	0.08083821092420751	9.230544538983562
>500	0.005374285489991496	2.0514089728297455
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	894	0.11815940946729138	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	799	0.10560332009436893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	784	0.10362077966706539	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	783	0.10348861030524514	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	773	0.10216691668704278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3216936182023644E-4
2	0.0	0.0	0.0	0.0	1.3216936182023644E-4
3	0.0	0.0	0.0	0.0	1.3216936182023644E-4
4	0.0	0.0	0.0	0.0	1.3216936182023644E-4
5	0.0	0.0	0.0	0.0	1.3216936182023644E-4
6	0.0	0.0	0.0	0.0	1.3216936182023644E-4
7	0.0	0.0	0.0	0.0	1.3216936182023644E-4
8	0.0	0.0	0.0	0.0	1.3216936182023644E-4
9	0.0	0.0	0.0	0.0	1.3216936182023644E-4
10	1.3216936182023644E-4	0.0	0.0	0.0	1.3216936182023644E-4
11	1.3216936182023644E-4	0.0	0.0	0.0	1.3216936182023644E-4
12	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
13	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
14	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
15	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
16	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
17	1.3216936182023644E-4	0.0	0.0	0.0	3.9650808546070937E-4
18	1.3216936182023644E-4	0.0	0.0	5.286774472809458E-4	6.608468091011823E-4
19	1.3216936182023644E-4	0.0	0.0	6.608468091011823E-4	6.608468091011823E-4
20	1.3216936182023644E-4	0.0	0.0	7.930161709214187E-4	9.251855327416552E-4
21	1.3216936182023644E-4	0.0	0.0	0.0013216936182023646	9.251855327416552E-4
22	1.3216936182023644E-4	0.0	0.0	0.0025112178745844926	9.251855327416552E-4
23	1.3216936182023644E-4	0.0	0.0	0.004229419578247566	9.251855327416552E-4
24	1.3216936182023644E-4	0.0	0.0	0.0077979923473939505	9.251855327416552E-4
25	1.3216936182023644E-4	0.0	0.0	0.01004487149833797	9.251855327416552E-4
26	1.3216936182023644E-4	0.0	0.0	0.012952597458383171	9.251855327416552E-4
27	1.3216936182023644E-4	0.0	0.0	0.015728154056608136	9.251855327416552E-4
28	1.3216936182023644E-4	0.0	0.0	0.023922654489462798	9.251855327416552E-4
29	1.3216936182023644E-4	0.0	0.0	0.037536098756947155	9.251855327416552E-4
30	1.3216936182023644E-4	0.0	0.0	0.06106224516094924	9.251855327416552E-4
31	1.3216936182023644E-4	0.0	0.0	0.11062575584353791	9.251855327416552E-4
32	1.3216936182023644E-4	0.0	0.0	0.1642865167425539	9.251855327416552E-4
33	1.3216936182023644E-4	0.0	0.0	0.21120664018873786	9.251855327416552E-4
34	1.3216936182023644E-4	0.0	0.0	0.2729297321587883	9.251855327416552E-4
35	1.3216936182023644E-4	0.0	0.0	0.3349171628524792	9.251855327416552E-4
36	1.3216936182023644E-4	0.0	0.0	0.42003423186471145	9.251855327416552E-4
37	1.3216936182023644E-4	0.0	0.0	0.5465203111266778	9.251855327416552E-4
38	1.3216936182023644E-4	0.0	0.0	0.705387884034602	0.0010573548945618915
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	60	9.747253E-6	25.666475	6
CCGGTAG	55	1.5929315E-4	23.99982	4
CGTCGTA	195	0.0	21.435736	10
GTATAGA	145	1.2732926E-11	21.24543	1
CCGTCGT	190	0.0	20.841948	9
TTAACGG	75	5.469483E-5	20.533178	35
ACCGTCG	210	0.0	19.904612	8
ATACCGT	200	0.0	19.79985	6
CTAGCGG	245	0.0	19.754953	29
TACCGTC	195	0.0	19.179342	7
AATACGA	245	0.0	18.857002	39
GTAAACG	175	9.094947E-12	18.857	27
TAGAACA	225	0.0	18.57764	4
TCTAGCG	250	0.0	18.47986	28
CGCAATA	250	0.0	18.47986	36
GTATAGT	60	0.0074014547	18.336832	1
CGGGATT	60	0.0074099246	18.333197	44
CGGTCCA	265	0.0	18.264015	10
CCCGTTT	110	2.7938222E-6	17.999865	22
GCAATAC	270	0.0	17.92579	37
>>END_MODULE