FastQCFastQC Report
Thu 2 Feb 2017
SRR4062493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617819
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG68980.4263764982362057No Hit
TCCATGTACTCTGCGTTGATACCAC62300.38508634154995086No Hit
GAGTACATGGAAGCAGTGGTATCAA31500.1947065771881774No Hit
GTATCAACGCAGAGTACTTTTTTTT31340.19371759139928507No Hit
CATGTACTCTGCGTTGATACCACTG27570.17041461374850958No Hit
TATCAACGCAGAGTACTTTTTTTTT22930.141734025870632No Hit
GCTTCCATGTACTCTGCGTTGATAC20050.12393228167057006No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19930.12319054232890081No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19910.12306691910528929No Hit
GAATAGGACCGCGGTTCTATTTTGT19240.11892554111430265No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18750.11589677213581989No Hit
GCGCAAGACGGACCAGAGCGAAAGC18610.1150314095705391No Hit
CCCATGTACTCTGCGTTGATACCAC18070.1116935825330275No Hit
GAATAACGCCGCCGCATCGCCAGTC17720.10953017611982552No Hit
GCGTTGATACCACTGCTTCCATGTA16980.10495611684619849No Hit
GTACATGGGAAGCAGTGGTATCAAC16400.1013710433614638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC456.758356E-414.77898112
CCGCTAA400.00530243514.240581
GCGTTAA500.001507083313.2912071
ATTTTCG655.4422504E-513.15654815
AATCGTA957.3734554E-813.001863513
TCGGCGT2250.012.66848413
ACGGTAT2050.012.5124419
CGGTCGG2450.012.4087910
TGTACGA550.00306026812.09526518
CGTCTTA1103.802961E-812.09339315
GCGCACG550.00306808512.09114559
CGTTAAC550.003081627712.0840382
CGTCTTC3700.012.06817710
GTTCAAA4500.012.0253791
AAGAATT4850.011.95164716
AGAATTT4300.011.9345317
GCGTCCC2400.011.87817516
TAGCACC802.8732225E-511.8722924
GTCTTCG3450.011.84117811
GAATTTC4300.011.7135218
Produced by FastQC (version 0.11.5)