##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062493_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1617819
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17454548376549	32.0	32.0	32.0	32.0	32.0
2	30.82581116923463	32.0	32.0	32.0	32.0	32.0
3	30.814365513076556	32.0	32.0	32.0	32.0	32.0
4	30.885775231963525	32.0	32.0	32.0	32.0	32.0
5	30.670449537309178	32.0	32.0	32.0	32.0	32.0
6	34.30919219022647	36.0	36.0	36.0	32.0	36.0
7	34.201094807268305	36.0	36.0	36.0	32.0	36.0
8	34.1940501378708	36.0	36.0	36.0	32.0	36.0
9	34.37065951135448	36.0	36.0	36.0	32.0	36.0
10	34.03556887389751	36.0	36.0	36.0	32.0	36.0
11	34.35485242786739	36.0	36.0	36.0	32.0	36.0
12	34.12144065559868	36.0	36.0	36.0	32.0	36.0
13	34.257248184129374	36.0	36.0	36.0	32.0	36.0
14	34.154940076732935	36.0	36.0	36.0	32.0	36.0
15	34.08705918276396	36.0	36.0	36.0	32.0	36.0
16	34.08886037313198	36.0	36.0	36.0	32.0	36.0
17	34.00202680275111	36.0	36.0	36.0	32.0	36.0
18	34.016994484549876	36.0	36.0	36.0	32.0	36.0
19	34.01655129529323	36.0	36.0	36.0	32.0	36.0
20	33.99934232445039	36.0	36.0	36.0	32.0	36.0
21	33.96276715751267	36.0	36.0	36.0	32.0	36.0
22	33.922049376351744	36.0	36.0	36.0	32.0	36.0
23	33.86579462844731	36.0	36.0	36.0	32.0	36.0
24	33.84122513087063	36.0	36.0	36.0	32.0	36.0
25	33.35779713305382	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	75.0
5	149.0
6	259.0
7	77.0
8	235.0
9	270.0
10	192.0
11	55.0
12	126.0
13	89.0
14	212.0
15	287.0
16	468.0
17	620.0
18	946.0
19	1315.0
20	2049.0
21	3029.0
22	4593.0
23	6807.0
24	9547.0
25	13803.0
26	19155.0
27	24985.0
28	34346.0
29	45554.0
30	59460.0
31	81720.0
32	117642.0
33	168540.0
34	368136.0
35	653066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01355519811681	17.126790061334678	11.116028984683021	25.74362575586549
2	16.893048809283915	19.678556725756383	36.679284890398236	26.749109574561462
3	19.527054801929857	23.324586536113763	28.17562827039039	28.97273039156599
4	12.722865437496292	14.808589190686265	35.320740766385086	37.147804605432356
5	14.754076060072418	36.23562990957478	33.44001662082748	15.570277409525318
6	35.13493457342865	34.632300195413954	16.417802458752814	13.814962772404582
7	30.543329548012956	30.230855061814527	20.409804227188495	18.81601116298402
8	28.639780552491516	32.520262332322666	19.379580716186297	19.460376398999518
9	27.060374631567747	14.210348522682558	18.471974400918636	40.25730244483106
10	15.842163173717545	26.585194087338376	31.449909092146772	26.122733646797307
11	38.03015535608754	20.836342371276608	21.452444823990618	19.681057448645237
12	24.337105856343744	23.60479984749814	28.627829856447722	23.430264439710395
13	29.750641265637135	19.127259900553543	25.20079581916464	25.921303014644682
14	23.54333603154682	19.438090853056284	24.659195833364276	32.35937728203262
15	25.665190425242923	26.861818481090516	22.437054505424058	25.035936588242503
16	26.248690498510896	25.553486754620614	23.73995481639753	24.457867930470965
17	24.084466250136146	25.74584377135049	25.433940966205576	24.735749012307785
18	25.439772938963728	24.287076743882356	26.455135826292857	23.818014490861053
19	25.511604201389943	25.602521675678986	24.985099808076864	23.900774314854207
20	25.2690194991175	24.092995219906577	25.59293209593853	25.045053185037393
21	26.563238821895496	24.318024840507306	24.15011499317963	24.96862134441757
22	25.31624021566546	24.692704577319844	24.754730062242142	25.236325144772557
23	24.17520521609741	24.34343846983252	25.13193126576026	26.349425048309804
24	24.30986313002338	25.79646888581625	24.778082978583814	25.115585005576552
25	25.10920489921214	24.54427300915353	24.703082740736615	25.64343935089771
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	151.0
1	151.0
2	444.0
3	737.0
4	737.0
5	737.0
6	2094.0
7	3451.0
8	3451.0
9	3451.0
10	3710.5
11	3970.0
12	3970.0
13	3970.0
14	4216.5
15	4463.0
16	4463.0
17	4463.0
18	7276.5
19	10090.0
20	10090.0
21	10090.0
22	16306.5
23	22523.0
24	22523.0
25	22523.0
26	33786.5
27	45050.0
28	45050.0
29	45050.0
30	55998.5
31	66947.0
32	66947.0
33	66947.0
34	81768.0
35	96589.0
36	96589.0
37	96589.0
38	109234.0
39	121879.0
40	121879.0
41	121879.0
42	141909.5
43	161940.0
44	161940.0
45	161940.0
46	196388.5
47	230837.0
48	230837.0
49	230837.0
50	223884.0
51	216931.0
52	216931.0
53	216931.0
54	199344.5
55	181758.0
56	181758.0
57	181758.0
58	167263.0
59	152768.0
60	152768.0
61	152768.0
62	136350.0
63	119932.0
64	119932.0
65	119932.0
66	100903.5
67	81875.0
68	81875.0
69	81875.0
70	62191.0
71	42507.0
72	42507.0
73	42507.0
74	34069.0
75	25631.0
76	25631.0
77	25631.0
78	20972.5
79	16314.0
80	16314.0
81	16314.0
82	11461.5
83	6609.0
84	6609.0
85	6609.0
86	4771.0
87	2933.0
88	2933.0
89	2933.0
90	1962.0
91	991.0
92	991.0
93	991.0
94	595.5
95	200.0
96	200.0
97	200.0
98	471.5
99	743.0
100	743.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008468190817390572
2	0.0032760154257058427
3	0.005192175391684731
4	0.015514714563248422
5	0.035294430341095015
6	0.04567878112446448
7	0.06471675756064182
8	0.08628901008085578
9	0.0933973454385194
10	0.11725662759554684
11	0.11459872828789872
12	0.12986619640392405
13	0.11583496052401411
14	0.11960546884416613
15	0.10990104579066014
16	0.10792307421287547
17	0.11935822239694303
18	0.1262193113073836
19	0.1278264132143336
20	0.12059445463305846
21	0.1280118480497509
22	0.14550453419078402
23	0.1362946040317242
24	0.13134967508726256
25	0.12665199259002397
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1617819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.966668261230694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11531335956094	39.53128617020495
2	12.586875024047558	12.578484175443089
3	3.666305886062625	5.495792698592659
4	1.4854049272978613	2.9688294094355894
5	0.7620342663782168	1.903815669590533
6	0.46656657579689614	1.3987666388773112
7	0.3189276306076606	1.1155025782539314
8	0.21428751815937055	0.8565786665913374
9	0.17285889310232125	0.7773464670882503
>10	0.9829576346890826	9.248076184480695
>50	0.10421648670105592	3.6553799595700145
>100	0.10517438905971385	10.931729907476116
>500	0.013255082554745225	4.412299868456065
>1k	0.005574567429565749	4.313510106660744
>5k	2.477585524251444E-4	0.8126014992788075
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6898	0.4263764982362057	No Hit
TCCATGTACTCTGCGTTGATACCAC	6230	0.38508634154995086	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3150	0.1947065771881774	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3134	0.19371759139928507	No Hit
CATGTACTCTGCGTTGATACCACTG	2757	0.17041461374850958	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2293	0.141734025870632	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2005	0.12393228167057006	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1993	0.12319054232890081	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1991	0.12306691910528929	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1924	0.11892554111430265	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1875	0.11589677213581989	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1861	0.1150314095705391	No Hit
CCCATGTACTCTGCGTTGATACCAC	1807	0.1116935825330275	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1772	0.10953017611982552	No Hit
GCGTTGATACCACTGCTTCCATGTA	1698	0.10495611684619849	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1640	0.1013710433614638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.236232236115412E-4
2	0.0	0.0	0.0	0.0	1.236232236115412E-4
3	0.0	0.0	0.0	0.0	1.236232236115412E-4
4	0.0	0.0	0.0	0.0	1.236232236115412E-4
5	0.0	0.0	0.0	0.0	1.236232236115412E-4
6	6.18116118057706E-5	0.0	0.0	0.0	2.472464472230824E-4
7	6.18116118057706E-5	0.0	0.0	1.236232236115412E-4	2.472464472230824E-4
8	6.18116118057706E-5	0.0	0.0	1.236232236115412E-4	2.472464472230824E-4
9	6.18116118057706E-5	0.0	0.0	2.472464472230824E-4	2.472464472230824E-4
10	1.8543483541731183E-4	0.0	0.0	2.472464472230824E-4	2.472464472230824E-4
11	1.8543483541731183E-4	6.18116118057706E-5	0.0	2.472464472230824E-4	2.472464472230824E-4
12	1.8543483541731183E-4	6.18116118057706E-5	0.0	3.0905805902885306E-4	3.0905805902885306E-4
13	1.8543483541731183E-4	6.18116118057706E-5	0.0	3.0905805902885306E-4	3.0905805902885306E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	45	6.758356E-4	14.778981	12
CCGCTAA	40	0.005302435	14.24058	1
GCGTTAA	50	0.0015070833	13.291207	1
ATTTTCG	65	5.4422504E-5	13.156548	15
AATCGTA	95	7.3734554E-8	13.0018635	13
TCGGCGT	225	0.0	12.668484	13
ACGGTAT	205	0.0	12.512441	9
CGGTCGG	245	0.0	12.40879	10
TGTACGA	55	0.003060268	12.095265	18
CGTCTTA	110	3.802961E-8	12.093393	15
GCGCACG	55	0.003068085	12.0911455	9
CGTTAAC	55	0.0030816277	12.084038	2
CGTCTTC	370	0.0	12.068177	10
GTTCAAA	450	0.0	12.025379	1
AAGAATT	485	0.0	11.951647	16
AGAATTT	430	0.0	11.93453	17
GCGTCCC	240	0.0	11.878175	16
TAGCACC	80	2.8732225E-5	11.872292	4
GTCTTCG	345	0.0	11.841178	11
GAATTTC	430	0.0	11.71352	18
>>END_MODULE