FastQCFastQC Report
Thu 2 Feb 2017
SRR4062493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617819
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32030.19798259261388323No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26980.1667677286519691No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21400.1322768492643491No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18840.11645307664207183No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18600.11496959795873334No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17890.11058097352052362No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16430.10155647819688111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG1650.026.6664629
CGCTCGA551.5940271E-423.99981510
ATTCGTG2000.019.79984744
TAATACT3000.018.3331934
GTCACGC2050.018.2437618
GATATAC3750.018.190461
GGTCACG2200.017.999867
TACACTA1601.1023076E-917.8748635
CGTCGTA3650.017.47931710
CGGTCCA6100.017.31134210
AATTCGT902.2160857E-417.1109832
ATACCGT4200.016.7617766
GCTAGTT2650.016.60364739
ATAACGT800.001988433816.5003833
TCTAATA2550.016.393552
CGCGCTT2151.2732926E-1116.37196512
GTAATAC1351.0857384E-616.2966733
GTCGATA1351.0860895E-616.2961736
CGCAATA5900.016.03377336
TAGCGGC6200.015.96761830
Produced by FastQC (version 0.11.5)