FastQCFastQC Report
Thu 2 Feb 2017
SRR4062492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074549
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30940.28793475216113923No Hit
TATCAACGCAGAGTACTTTTTTTTT21340.19859494541430872No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19620.1825882300388349No Hit
GTACATGGAAGCAGTGGTATCAACG18410.17132769189678645No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16870.15699609789781574No Hit
GCGCAAGACGGACCAGAGCGAAAGC16200.15076092388527654No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15140.14089632022364731No Hit
TCCATGTACTCTGCGTTGATACCAC14740.1371738282758627No Hit
GAATAGGACCGCGGTTCTATTTTGT14620.13605708069152733No Hit
GGGTAGGCACACGCTGAGCCAGTCA14580.13568483149674887No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14540.1353125823019704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13370.12442429335470044No Hit
GAACTACGACGGTATCTGATCGTCT12850.11958505382258044No Hit
GAATAACGCCGCCGCATCGCCAGTC12810.11921280462780198No Hit
GTATCTGATCGTCTTCGAACCTCCG12370.11511806348523893No Hit
GATTAAGAGGGACGGCCGGGGGCAT12270.11418744049829278No Hit
GTCCTATTCCATTATTCCTAGCTGC12010.11176782073223276No Hit
GTACATGGGGAATAATTGCAATCCC11530.10730083039489126No Hit
GGTATCAACGCAGAGTACTTTTTTT11370.10581183361577742No Hit
CTATTGGAGCTGGAATTACCGCGGC11240.10460202373274742No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC11240.10460202373274742No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11180.10404364994057971No Hit
TCGTAGTTCCGACCATAAACGATGC10790.10041422029148973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC504.575546E-617.0937541
GTCTAGG350.002174913216.2797641
CGAACGA1600.014.84593516
GCGTTAT1101.2732926E-1114.67645551
ACGAACG1650.014.39471615
AACGAAC1600.014.25076914
GTTATTC1301.8189894E-1213.8827783
TAGAGTG1400.013.5702635
CCTTTAG707.2849816E-613.566471
ATAACGA1700.013.41248912
CGATAAC1700.013.41186310
CGCATCG2300.013.21810413
CGGACAT1153.765308E-1013.2162575
TATACAT655.4538097E-513.1527185
GCATCGC2400.013.06320614
AATCGCT801.9965482E-613.06320515
TCAGGAC1251.0186341E-1012.918293
ATCGCTC751.4753401E-512.6685316
GAACCGC1501.8189894E-1212.667356
GATAACG1800.012.6667611
Produced by FastQC (version 0.11.5)