##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062492_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1074549
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.110273240215196	32.0	32.0	32.0	32.0	32.0
2	30.748017075070564	32.0	32.0	32.0	32.0	32.0
3	30.742708801553025	32.0	32.0	32.0	32.0	32.0
4	30.808274913475326	32.0	32.0	32.0	32.0	32.0
5	30.59503382349246	32.0	32.0	32.0	32.0	32.0
6	34.24539597542783	36.0	36.0	36.0	32.0	36.0
7	34.088826102858036	36.0	36.0	36.0	32.0	36.0
8	34.07902478155952	36.0	36.0	36.0	32.0	36.0
9	34.270036080253206	36.0	36.0	36.0	32.0	36.0
10	33.90394574840235	36.0	36.0	36.0	32.0	36.0
11	34.26602416455648	36.0	36.0	36.0	32.0	36.0
12	34.01803733473299	36.0	36.0	36.0	32.0	36.0
13	34.160891685721175	36.0	36.0	36.0	32.0	36.0
14	34.070693844580376	36.0	36.0	36.0	32.0	36.0
15	33.993436316073065	36.0	36.0	36.0	32.0	36.0
16	33.98956399382438	36.0	36.0	36.0	32.0	36.0
17	33.90337155401941	36.0	36.0	36.0	32.0	36.0
18	33.94165459183341	36.0	36.0	36.0	32.0	36.0
19	33.92014510273612	36.0	36.0	36.0	32.0	36.0
20	33.90301698666138	36.0	36.0	36.0	32.0	36.0
21	33.88418862238949	36.0	36.0	36.0	32.0	36.0
22	33.82653373647921	36.0	36.0	36.0	32.0	36.0
23	33.80159583229801	36.0	36.0	36.0	32.0	36.0
24	33.7705642087983	36.0	36.0	36.0	32.0	36.0
25	33.299527522709525	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	39.0
5	112.0
6	187.0
7	36.0
8	160.0
9	234.0
10	137.0
11	38.0
12	83.0
13	57.0
14	213.0
15	286.0
16	450.0
17	634.0
18	828.0
19	1181.0
20	1653.0
21	2619.0
22	3749.0
23	5432.0
24	7410.0
25	10217.0
26	13753.0
27	17825.0
28	23616.0
29	31032.0
30	39476.0
31	54192.0
32	77032.0
33	110560.0
34	241917.0
35	429379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.741711068196096	16.804299774308383	10.780520719421112	25.67346843807441
2	17.283842615945012	19.53769038769011	36.13545794486458	27.0430090515003
3	19.62728676341253	23.357862594567326	27.60886701616288	29.405983625857264
4	12.771447000645022	14.498487966559349	34.75405838338541	37.97600664941022
5	15.150220265957644	36.21997177318751	32.65801243022429	15.971795530630562
6	35.55939150003119	34.159923504563565	16.33246465949941	13.948220335905837
7	31.289206848253663	29.882975473834783	20.043825992370962	18.783991685540588
8	29.266611460470905	31.806034366322606	19.071939758800465	19.855414414406024
9	27.48334376676802	13.95611622846241	18.52745111190604	40.03308889286353
10	16.598632356627125	25.88925238042438	30.652337376879018	26.859777886069473
11	38.29741835153541	20.82696237242937	21.427020701080572	19.448598574954644
12	24.983738670652635	23.394787801300534	27.902391021136935	23.7190825069099
13	29.67520051881448	18.663810453813586	25.3066484286481	26.354340598723837
14	23.95586599154294	19.32470233491678	24.44124317685844	32.27818849668184
15	25.539085064753564	26.814031491661233	22.118978850274853	25.527904593310353
16	26.51680389232018	25.21184802286049	23.405351567558906	24.86599651726043
17	24.408750336612332	25.55551517760552	24.69518530217771	25.340549183604438
18	25.5007790310196	24.053514585398947	26.10024153689029	24.34546484669117
19	25.84331438230873	24.76337622085942	24.931484637742205	24.46182475908964
20	25.8482077964674	23.643407963444044	24.937104222496377	25.57128001759218
21	27.19504478941566	23.759123985563765	24.03746786279054	25.00836336223003
22	26.24247728389055	23.792054744216003	24.537871988233945	25.4275959836595
23	24.425619160783764	23.66981198014958	25.459984622911865	26.444584236154796
24	24.95175170884218	24.841509838364733	24.901429975910798	25.30530847688229
25	25.101108182960147	24.254688251322325	24.892089146665572	25.752114419051953
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	206.0
2	482.0
3	758.0
4	758.0
5	758.0
6	1888.5
7	3019.0
8	3019.0
9	3019.0
10	3080.0
11	3141.0
12	3141.0
13	3141.0
14	2963.0
15	2785.0
16	2785.0
17	2785.0
18	4457.0
19	6129.0
20	6129.0
21	6129.0
22	9727.0
23	13325.0
24	13325.0
25	13325.0
26	20107.0
27	26889.0
28	26889.0
29	26889.0
30	33158.5
31	39428.0
32	39428.0
33	39428.0
34	49333.0
35	59238.0
36	59238.0
37	59238.0
38	68722.0
39	78206.0
40	78206.0
41	78206.0
42	91647.5
43	105089.0
44	105089.0
45	105089.0
46	123185.5
47	141282.0
48	141282.0
49	141282.0
50	142990.0
51	144698.0
52	144698.0
53	144698.0
54	135063.5
55	125429.0
56	125429.0
57	125429.0
58	117925.0
59	110421.0
60	110421.0
61	110421.0
62	98901.0
63	87381.0
64	87381.0
65	87381.0
66	73030.0
67	58679.0
68	58679.0
69	58679.0
70	44650.5
71	30622.0
72	30622.0
73	30622.0
74	24227.5
75	17833.0
76	17833.0
77	17833.0
78	14767.5
79	11702.0
80	11702.0
81	11702.0
82	8220.0
83	4738.0
84	4738.0
85	4738.0
86	3474.5
87	2211.0
88	2211.0
89	2211.0
90	1439.5
91	668.0
92	668.0
93	668.0
94	398.5
95	129.0
96	129.0
97	129.0
98	336.0
99	543.0
100	543.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0068866101034015205
2	0.002884931259533069
3	0.004653114934730757
4	0.01563446618069534
5	0.03657348338698375
6	0.04634502474991834
7	0.07044816011182366
8	0.09287617409722591
9	0.10022809569410052
10	0.12246998508211353
11	0.12284223427689199
12	0.13503339540588655
13	0.12405204415992198
14	0.12684391312076043
15	0.11623481106957431
16	0.11688624716043661
17	0.12619247702989814
18	0.1322415264450481
19	0.13326521173068887
20	0.12516879174425735
21	0.13177621495157504
22	0.1518776714696119
23	0.14182694321059347
24	0.1353125823019704
25	0.1341027724189404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1074549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.5094666559805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56303118102483	37.140318081567194
2	13.57001658690965	13.165485342875922
3	4.281205567602974	6.2303699618710375
4	1.839924302318642	3.5701498637141746
5	0.9520938996092011	2.309278363822749
6	0.6025861754470446	1.7538680391121937
7	0.38007440331610826	1.2906044616118109
8	0.2773711361543472	1.0764100704488588
9	0.2004363602522013	0.8750754840870231
>10	1.0999803225042168	9.825038836550279
>50	0.10340902927521581	3.529774438686606
>100	0.11222238700434181	11.67961323074095
>500	0.012852820162437316	4.197532541641232
>1k	0.004795828418819895	3.3564812832699493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3094	0.28793475216113923	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2134	0.19859494541430872	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1962	0.1825882300388349	No Hit
GTACATGGAAGCAGTGGTATCAACG	1841	0.17132769189678645	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1687	0.15699609789781574	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1620	0.15076092388527654	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1514	0.14089632022364731	No Hit
TCCATGTACTCTGCGTTGATACCAC	1474	0.1371738282758627	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1462	0.13605708069152733	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1458	0.13568483149674887	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1454	0.1353125823019704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1337	0.12442429335470044	No Hit
GAACTACGACGGTATCTGATCGTCT	1285	0.11958505382258044	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1281	0.11921280462780198	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1237	0.11511806348523893	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1227	0.11418744049829278	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1201	0.11176782073223276	No Hit
GTACATGGGGAATAATTGCAATCCC	1153	0.10730083039489126	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1137	0.10581183361577742	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1124	0.10460202373274742	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1124	0.10460202373274742	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1118	0.10404364994057971	No Hit
TCGTAGTTCCGACCATAAACGATGC	1079	0.10041422029148973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.791868960838454E-4
2	0.0	0.0	0.0	0.0	2.791868960838454E-4
3	0.0	0.0	0.0	0.0	2.791868960838454E-4
4	0.0	0.0	0.0	0.0	2.791868960838454E-4
5	0.0	0.0	0.0	0.0	4.653114934730757E-4
6	0.0	0.0	0.0	0.0	4.653114934730757E-4
7	0.0	0.0	0.0	0.0	4.653114934730757E-4
8	0.0	0.0	0.0	9.306229869461514E-5	4.653114934730757E-4
9	0.0	0.0	0.0	2.791868960838454E-4	4.653114934730757E-4
10	0.0	0.0	0.0	2.791868960838454E-4	4.653114934730757E-4
11	0.0	0.0	0.0	2.791868960838454E-4	4.653114934730757E-4
12	0.0	0.0	0.0	2.791868960838454E-4	0.0010236852856407666
13	0.0	0.0	0.0	2.791868960838454E-4	0.0011167475843353817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	50	4.575546E-6	17.093754	1
GTCTAGG	35	0.0021749132	16.279764	1
CGAACGA	160	0.0	14.845935	16
GCGTTAT	110	1.2732926E-11	14.6764555	1
ACGAACG	165	0.0	14.394716	15
AACGAAC	160	0.0	14.250769	14
GTTATTC	130	1.8189894E-12	13.882778	3
TAGAGTG	140	0.0	13.570263	5
CCTTTAG	70	7.2849816E-6	13.56647	1
ATAACGA	170	0.0	13.412489	12
CGATAAC	170	0.0	13.411863	10
CGCATCG	230	0.0	13.218104	13
CGGACAT	115	3.765308E-10	13.216257	5
TATACAT	65	5.4538097E-5	13.152718	5
GCATCGC	240	0.0	13.063206	14
AATCGCT	80	1.9965482E-6	13.063205	15
TCAGGAC	125	1.0186341E-10	12.91829	3
ATCGCTC	75	1.4753401E-5	12.66853	16
GAACCGC	150	1.8189894E-12	12.66735	6
GATAACG	180	0.0	12.66676	11
>>END_MODULE