FastQCFastQC Report
Thu 2 Feb 2017
SRR4062492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074549
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16460.15318054365133651No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15590.145084123664905No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14430.13428889701632965No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13880.1291704705881258No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13880.1291704705881258No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13690.12740228691292813No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12070.11232619452440047No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11800.10981351245964587No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11640.10832451568053203No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11630.1082314533818374No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11350.10562570901838818No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10990.10227546626538203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC952.67828E-823.1631131
CGCAATA4600.021.04332436
CTAGCGG4500.021.02206629
TCTAGCG4400.020.99984628
CCGGTCC5000.020.6798469
ATACGAA4700.020.59559240
CAATACG4750.020.37879638
GTCCTAA1653.6379788E-1220.0045071
CTATAGC1001.1232787E-619.7998544
TAGCGGC4950.019.5554130
CGGTCCA5200.019.46139310
ATAACGC4100.018.7812233
GCGCAAT5200.018.61524635
AATACGA5200.018.61524639
GCAATAC5250.018.43795837
CGAATGC5150.018.36879543
TACGAAT5200.018.19217341
AATAACG4150.018.0248012
GTTATAT1102.7898295E-618.0040551
TAGGACG2100.017.8093914
Produced by FastQC (version 0.11.5)