##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1074549
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22787048333766	32.0	32.0	32.0	32.0	32.0
2	31.40222083869605	32.0	32.0	32.0	32.0	32.0
3	31.49072215413164	32.0	32.0	32.0	32.0	32.0
4	31.603592763103403	32.0	32.0	32.0	32.0	32.0
5	31.527407312277056	32.0	32.0	32.0	32.0	32.0
6	35.13020160085766	36.0	36.0	36.0	36.0	36.0
7	35.144733278798824	36.0	36.0	36.0	36.0	36.0
8	35.08200463636372	36.0	36.0	36.0	36.0	36.0
9	35.20574771369198	36.0	36.0	36.0	36.0	36.0
10	35.08345920009232	36.0	36.0	36.0	36.0	36.0
11	35.21994436735784	36.0	36.0	36.0	36.0	36.0
12	35.110625015704265	36.0	36.0	36.0	36.0	36.0
13	35.15474399026941	36.0	36.0	36.0	36.0	36.0
14	35.114153938070764	36.0	36.0	36.0	36.0	36.0
15	35.09197533104586	36.0	36.0	36.0	36.0	36.0
16	35.090323475244034	36.0	36.0	36.0	36.0	36.0
17	35.0586795018189	36.0	36.0	36.0	36.0	36.0
18	35.07904432464225	36.0	36.0	36.0	36.0	36.0
19	35.05878931533136	36.0	36.0	36.0	36.0	36.0
20	35.03729843869382	36.0	36.0	36.0	36.0	36.0
21	35.031068848419196	36.0	36.0	36.0	36.0	36.0
22	35.027568775365296	36.0	36.0	36.0	36.0	36.0
23	34.987579905616215	36.0	36.0	36.0	36.0	36.0
24	34.959409947801355	36.0	36.0	36.0	32.0	36.0
25	34.93503041741233	36.0	36.0	36.0	32.0	36.0
26	34.89463951853289	36.0	36.0	36.0	32.0	36.0
27	34.877868761685136	36.0	36.0	36.0	32.0	36.0
28	34.86346737096214	36.0	36.0	36.0	32.0	36.0
29	34.8410635531744	36.0	36.0	36.0	32.0	36.0
30	34.81221144871011	36.0	36.0	36.0	32.0	36.0
31	34.80397171278369	36.0	36.0	36.0	32.0	36.0
32	34.76892352047231	36.0	36.0	36.0	32.0	36.0
33	34.746649059279754	36.0	36.0	36.0	32.0	36.0
34	34.73035198953235	36.0	36.0	36.0	32.0	36.0
35	34.69029239243627	36.0	36.0	36.0	32.0	36.0
36	34.659511106520036	36.0	36.0	36.0	32.0	36.0
37	34.65369843534357	36.0	36.0	36.0	32.0	36.0
38	34.60836313653449	36.0	36.0	36.0	32.0	36.0
39	34.57842592566742	36.0	36.0	36.0	32.0	36.0
40	34.573869595523334	36.0	36.0	36.0	32.0	36.0
41	34.53414781457151	36.0	36.0	36.0	32.0	36.0
42	34.488682228544256	36.0	36.0	36.0	32.0	36.0
43	34.485571155898896	36.0	36.0	36.0	32.0	36.0
44	34.43582749600065	36.0	36.0	36.0	32.0	36.0
45	34.373366872985784	36.0	36.0	36.0	32.0	36.0
46	34.37980492280948	36.0	36.0	36.0	32.0	36.0
47	34.333127665653215	36.0	36.0	36.0	32.0	36.0
48	34.29184150746034	36.0	36.0	36.0	32.0	36.0
49	34.278270232441706	36.0	36.0	36.0	32.0	36.0
50	33.76807013919328	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	28.0
22	100.0
23	296.0
24	755.0
25	1761.0
26	3753.0
27	7018.0
28	11819.0
29	17493.0
30	25154.0
31	35432.0
32	52411.0
33	84802.0
34	186987.0
35	646736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06062986556068	17.41205225672662	11.300107596138453	26.227210281574255
2	16.648270444934184	19.975783995473225	35.827494937161234	27.548450622431353
3	18.997639929123423	23.457671347101655	28.12048645751128	29.424202266263645
4	12.526592626095114	14.893266551641343	34.89603981588503	37.684101006378505
5	14.837015343181188	36.36483771331042	32.63313259795505	16.165014345553345
6	35.8209024673745	34.272430233131665	16.006990878913175	13.899676420580656
7	31.1891779714094	29.901847193566788	20.103410826309457	18.805564008714352
8	29.239988125250687	31.92250888512297	18.969912028209045	19.867590961417303
9	27.495628398518818	13.620504974645176	18.41302723282047	40.470839394015535
10	16.47286442963513	25.924550671956325	30.692039171782763	26.910545726625774
11	38.53858688621924	20.62297763992149	21.3365793463118	19.501856127547466
12	24.986296576517216	23.36087046751707	27.86880821628423	23.784024739681485
13	29.903708439540683	18.49836536072343	25.335745508115497	26.26218069162039
14	23.906215537867514	19.104852361316237	24.509910669499483	32.47902143131677
15	25.714043752309106	26.63470907329494	22.04617937385824	25.60506780053771
16	26.449608161191346	25.15995082588137	23.429085132460223	24.96135588046706
17	24.444208686621085	25.497022471753265	24.707574991926844	25.351193849698806
18	25.720185864022955	23.766249840630813	26.119609250020243	24.393955045325992
19	25.853730262649727	24.6409423860615	24.970941297232606	24.534386054056167
20	25.969872011420602	23.359288408439262	25.01477363991777	25.656065940222362
21	27.30466456159747	23.63047194683537	24.12733155956592	24.93753193200124
22	26.16316240580932	23.59073434529277	24.819435874957772	25.426667373940138
23	24.44709361788062	23.451513146445624	25.579661792994084	26.521731442679673
24	25.091550036340827	24.686356787824472	24.93353025315737	25.288562922677325
25	25.211321214760794	23.962239041681674	25.013191580839962	25.813248162717567
26	24.88048474290144	24.80184710050449	25.602275931576873	24.715392225017194
27	25.811293854444983	24.121840883942937	24.802963848088826	25.263901413523254
28	24.83655933791758	23.944929454124477	25.62377332257533	25.594737885382614
29	24.544809031509963	24.370689470652337	25.687334872583754	25.39716662525394
30	24.559140625508935	24.57775308524786	25.94139494802005	24.921711341223155
31	25.217742513370727	24.575240403183102	24.458168031425277	25.748849052020894
32	25.050137313421722	24.33541885944708	24.540435103471317	26.07400872365988
33	24.544436782315184	23.99304266254959	25.284468181534763	26.17805237360046
34	25.51340143632352	24.243380246038104	25.42164200981063	24.82157630782775
35	26.005328747223256	23.951816064227877	25.484738248325577	24.558116940223293
36	24.714647726627636	24.85787060431865	24.844004321813152	25.583477347240564
37	25.975548811640977	24.501628124915662	24.773463099402633	24.74935996404073
38	24.909240993198075	24.180004820627072	25.24184564873263	25.66890853744222
39	25.60804579409594	24.091037263075023	24.59152630545466	25.70939063737438
40	25.705388958530513	24.432762023881647	25.124959401572195	24.73688961601565
41	24.331882492096685	24.872109136018924	25.842469724507676	24.953538647376714
42	26.315226201876325	24.91389410813281	24.822320806217306	23.948558883773565
43	25.14087305464897	23.621258779264604	25.20471379155348	26.033154374532945
44	24.660392406488675	24.598040666363282	25.041203332747042	25.700363594400997
45	24.911195301470663	24.71511303812111	24.856009358344757	25.51768230206347
46	24.514563784434216	24.373016028119704	25.337048380297222	25.77537180714886
47	24.915848416405396	24.184657935561805	25.64992382850852	25.249569819524286
48	25.78988952574522	25.16851255736127	24.12463275290378	24.91696516398973
49	24.453887165685327	25.106533066430657	24.86996870314895	25.569611064735064
50	24.417220619999647	25.393444133306158	24.606974647037966	25.58236059965623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	22.0
2	24.0
3	96.5
4	169.0
5	190.5
6	212.0
7	216.0
8	220.0
9	288.0
10	356.0
11	522.0
12	688.0
13	1235.0
14	1782.0
15	2446.5
16	3111.0
17	3657.0
18	4203.0
19	4411.0
20	4619.0
21	4963.0
22	5307.0
23	5588.5
24	5870.0
25	6077.5
26	6285.0
27	7499.0
28	8713.0
29	9813.0
30	10913.0
31	12301.5
32	13690.0
33	15802.0
34	17914.0
35	21061.5
36	24209.0
37	29323.5
38	34438.0
39	37783.0
40	41128.0
41	45393.5
42	49659.0
43	51767.5
44	53876.0
45	61192.0
46	68508.0
47	74151.5
48	79795.0
49	82564.5
50	85334.0
51	80600.5
52	75867.0
53	75569.0
54	75271.0
55	77305.0
56	79339.0
57	77938.0
58	76537.0
59	70392.5
60	64248.0
61	57373.5
62	50499.0
63	44039.0
64	37579.0
65	32528.0
66	27477.0
67	23975.5
68	20474.0
69	18813.5
70	17153.0
71	13319.0
72	9485.0
73	8694.0
74	7903.0
75	5659.5
76	3416.0
77	3168.5
78	2921.0
79	2567.5
80	2214.0
81	1772.5
82	1331.0
83	1106.0
84	881.0
85	719.0
86	557.0
87	413.0
88	269.0
89	157.0
90	45.0
91	30.0
92	15.0
93	13.0
94	11.0
95	8.0
96	5.0
97	5.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014983030089833036
2	0.004932301830814602
3	4.653114934730757E-4
4	2.791868960838454E-4
5	0.0
6	5.583737921676908E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1074549.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.71742019329986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03625646760777	39.84116445854085
2	13.637006902453514	14.105416323062375
3	4.084250482947896	6.336806952039126
4	1.7619370601387203	3.6449135717336647
5	0.9206672906284781	2.3807268563829957
6	0.5516195332364004	1.7117003512331315
7	0.3578767868816782	1.2955924915211439
8	0.24469211370686295	1.0123875890051628
9	0.1975721919097102	0.9196131660755185
>10	1.0066479904030157	9.443831628982199
>50	0.09153873211953953	3.3073390505471894
>100	0.09657623976432378	10.495536890601345
>500	0.010650463296233577	3.746378727189903
>1k	0.0027077449058220955	1.7585919430854544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1646	0.15318054365133651	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1559	0.145084123664905	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1443	0.13428889701632965	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1388	0.1291704705881258	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1388	0.1291704705881258	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1369	0.12740228691292813	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1207	0.11232619452440047	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1180	0.10981351245964587	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1164	0.10832451568053203	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1163	0.1082314533818374	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1135	0.10562570901838818	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1099	0.10227546626538203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8612459738923028E-4
2	0.0	0.0	0.0	0.0	1.8612459738923028E-4
3	0.0	0.0	0.0	0.0	1.8612459738923028E-4
4	0.0	0.0	0.0	0.0	1.8612459738923028E-4
5	0.0	0.0	0.0	0.0	1.8612459738923028E-4
6	0.0	0.0	0.0	0.0	1.8612459738923028E-4
7	0.0	0.0	0.0	0.0	1.8612459738923028E-4
8	0.0	0.0	0.0	0.0	1.8612459738923028E-4
9	0.0	0.0	0.0	0.0	1.8612459738923028E-4
10	0.0	0.0	0.0	0.0	2.791868960838454E-4
11	0.0	0.0	0.0	0.0	2.791868960838454E-4
12	0.0	0.0	0.0	0.0	4.653114934730757E-4
13	0.0	0.0	0.0	0.0	5.583737921676908E-4
14	0.0	0.0	0.0	0.0	5.583737921676908E-4
15	0.0	0.0	0.0	0.0	7.444983895569211E-4
16	0.0	0.0	0.0	2.791868960838454E-4	7.444983895569211E-4
17	0.0	0.0	0.0	2.791868960838454E-4	7.444983895569211E-4
18	0.0	0.0	0.0	3.7224919477846057E-4	8.375606882515363E-4
19	0.0	0.0	0.0	3.7224919477846057E-4	9.306229869461514E-4
20	0.0	0.0	0.0	5.583737921676908E-4	0.0010236852856407666
21	0.0	0.0	0.0	8.375606882515363E-4	0.0012098098830299968
22	0.0	0.0	0.0	0.002047370571281533	0.0012098098830299968
23	0.0	0.0	0.0	0.0036294296490899905	0.001302872181724612
24	0.0	0.0	0.0	0.0068866101034015205	0.0013959344804192271
25	0.0	0.0	0.0	0.009492354466850744	0.0013959344804192271
26	0.0	0.0	0.0	0.011353600440743046	0.0013959344804192271
27	9.306229869461514E-5	0.0	0.0	0.014331593998970731	0.0013959344804192271
28	9.306229869461514E-5	0.0	0.0	0.022893325478875324	0.0013959344804192271
29	9.306229869461514E-5	0.0	0.0	0.03759716867262451	0.0013959344804192271
30	9.306229869461514E-5	0.0	0.0	0.06216561552800291	0.0013959344804192271
31	9.306229869461514E-5	0.0	0.0	0.11176782073223278	0.0013959344804192271
32	9.306229869461514E-5	0.0	0.0	0.1722583148837326	0.0014889967791138423
33	9.306229869461514E-5	0.0	0.0	0.2386117338529932	0.0014889967791138423
34	9.306229869461514E-5	0.0	0.0	0.30087041167969075	0.0015820590778084574
35	9.306229869461514E-5	0.0	0.0	0.38015949016750283	0.0015820590778084574
36	9.306229869461514E-5	0.0	0.0	0.49155506170495716	0.0015820590778084574
37	9.306229869461514E-5	0.0	0.0	0.6448286676549883	0.0015820590778084574
38	9.306229869461514E-5	0.0	0.0	0.8469599804196923	0.0015820590778084574
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	95	2.67828E-8	23.163113	1
CGCAATA	460	0.0	21.043324	36
CTAGCGG	450	0.0	21.022066	29
TCTAGCG	440	0.0	20.999846	28
CCGGTCC	500	0.0	20.679846	9
ATACGAA	470	0.0	20.595592	40
CAATACG	475	0.0	20.378796	38
GTCCTAA	165	3.6379788E-12	20.004507	1
CTATAGC	100	1.1232787E-6	19.799854	4
TAGCGGC	495	0.0	19.55541	30
CGGTCCA	520	0.0	19.461393	10
ATAACGC	410	0.0	18.781223	3
GCGCAAT	520	0.0	18.615246	35
AATACGA	520	0.0	18.615246	39
GCAATAC	525	0.0	18.437958	37
CGAATGC	515	0.0	18.368795	43
TACGAAT	520	0.0	18.192173	41
AATAACG	415	0.0	18.024801	2
GTTATAT	110	2.7898295E-6	18.004055	1
TAGGACG	210	0.0	17.809391	4
>>END_MODULE