FastQCFastQC Report
Thu 2 Feb 2017
SRR4062491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643708
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31300.19042311651461208No Hit
GCGCAAGACGGACCAGAGCGAAAGC19360.11778247717964503No Hit
TATCAACGCAGAGTACTTTTTTTTT18940.11522727881107836No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18430.11212453793496169No Hit
GAATAGGACCGCGGTTCTATTTTGT17380.10573654201354499No Hit
GAATAACGCCGCCGCATCGCCAGTC17380.10573654201354499No Hit
GATTAAGAGGGACGGCCGGGGGCAT17310.10531067561878388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17290.10518899950599499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACT400.005272254314.2528578
TTAGGGT852.7047645E-713.4095514
TAGTGCG500.001497347113.3026668
GCATCGC3500.013.30063914
CGCATCG3650.013.01472213
GCTCGTA1207.385097E-1012.6688199
CGAACGA2250.012.66650316
CGCCAGT3700.012.58245318
ATCGCCA3900.012.17933216
AACCGCG1251.4060788E-912.16243657
TCGCCAG3850.012.09112217
GTATTAG3700.011.8038281
CGTATGC907.4470645E-611.6130859
CCGCATC4100.011.58698212
CGGTCCA3700.011.55601810
CGAGGTT1900.011.4981014
ACGAACG2400.011.47971815
AGTTATG1251.826993E-811.40193710
CATCGCC4200.011.31006815
CGTCTTA1354.7239155E-911.25980115
Produced by FastQC (version 0.11.5)