FastQCFastQC Report
Thu 2 Feb 2017
SRR4062491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643708
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18610.11321962295006169No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18360.11169867154020058No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17490.10640576063388388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG2300.020.08691435
GTATCAA25450.020.0567991
TAACGGC2450.018.85710536
GTAAGAC1102.797724E-617.9999623
TATACCG902.2160164E-417.1110765
ATACCGG1307.320905E-716.9230426
CGTCTTA3150.016.7618715
GTATAAA2251.8189894E-1216.6237051
TAGGACG3850.016.5713944
AACGCAG31250.016.1919656
TAATACT4100.016.0975274
CAACGGA2201.8189894E-1115.99996614
TACCGTC5950.015.8991277
ATACCGT6050.015.6363326
CGTATTG6550.015.45035127
GCATTCG7050.015.29074822
ACCGTCG6050.015.2726968
GGCGTTA5200.015.23073842
CGTCGTA5850.015.04270510
TGTAGGA6300.015.01584152
Produced by FastQC (version 0.11.5)