##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1643708
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27030835160503	32.0	32.0	32.0	32.0	32.0
2	31.385834345273004	32.0	32.0	32.0	32.0	32.0
3	31.469175181966627	32.0	32.0	32.0	32.0	32.0
4	31.572444740793376	32.0	32.0	32.0	32.0	32.0
5	31.50761753304115	32.0	32.0	32.0	32.0	32.0
6	35.09052155248986	36.0	36.0	36.0	36.0	36.0
7	35.1010380189182	36.0	36.0	36.0	36.0	36.0
8	35.04042993037693	36.0	36.0	36.0	36.0	36.0
9	35.187406765678574	36.0	36.0	36.0	36.0	36.0
10	35.040503544425164	36.0	36.0	36.0	36.0	36.0
11	35.184209117434484	36.0	36.0	36.0	36.0	36.0
12	35.083292166248505	36.0	36.0	36.0	36.0	36.0
13	35.142162111518594	36.0	36.0	36.0	36.0	36.0
14	35.10108729774388	36.0	36.0	36.0	36.0	36.0
15	35.05734534357684	36.0	36.0	36.0	36.0	36.0
16	35.07610354150494	36.0	36.0	36.0	36.0	36.0
17	35.0404743421581	36.0	36.0	36.0	36.0	36.0
18	35.05752420746264	36.0	36.0	36.0	36.0	36.0
19	35.04079800061812	36.0	36.0	36.0	36.0	36.0
20	35.03823428492165	36.0	36.0	36.0	36.0	36.0
21	35.03480119339932	36.0	36.0	36.0	36.0	36.0
22	35.01631189968048	36.0	36.0	36.0	36.0	36.0
23	34.97413409194334	36.0	36.0	36.0	32.0	36.0
24	34.950509457884245	36.0	36.0	36.0	32.0	36.0
25	34.92843862778547	36.0	36.0	36.0	32.0	36.0
26	34.88402319633414	36.0	36.0	36.0	32.0	36.0
27	34.870250068747005	36.0	36.0	36.0	32.0	36.0
28	34.85732928232995	36.0	36.0	36.0	32.0	36.0
29	34.833161364427255	36.0	36.0	36.0	32.0	36.0
30	34.81926838586902	36.0	36.0	36.0	32.0	36.0
31	34.82564117227634	36.0	36.0	36.0	32.0	36.0
32	34.78369759105632	36.0	36.0	36.0	32.0	36.0
33	34.76584831369075	36.0	36.0	36.0	32.0	36.0
34	34.759850289710826	36.0	36.0	36.0	32.0	36.0
35	34.737777634470355	36.0	36.0	36.0	32.0	36.0
36	34.70440248511293	36.0	36.0	36.0	32.0	36.0
37	34.70132590460106	36.0	36.0	36.0	32.0	36.0
38	34.67440080598257	36.0	36.0	36.0	32.0	36.0
39	34.666825251200336	36.0	36.0	36.0	32.0	36.0
40	34.66793797925179	36.0	36.0	36.0	32.0	36.0
41	34.63347017840152	36.0	36.0	36.0	32.0	36.0
42	34.60508679157125	36.0	36.0	36.0	32.0	36.0
43	34.603242181701376	36.0	36.0	36.0	32.0	36.0
44	34.56508820301416	36.0	36.0	36.0	32.0	36.0
45	34.53406809481976	36.0	36.0	36.0	32.0	36.0
46	34.53331674482329	36.0	36.0	36.0	32.0	36.0
47	34.51667510287715	36.0	36.0	36.0	32.0	36.0
48	34.47847245374483	36.0	36.0	36.0	32.0	36.0
49	34.45499261425995	36.0	36.0	36.0	32.0	36.0
50	33.92682520252989	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	4.0
21	28.0
22	126.0
23	397.0
24	988.0
25	2335.0
26	5012.0
27	9225.0
28	15753.0
29	24882.0
30	36928.0
31	53896.0
32	79314.0
33	133073.0
34	293514.0
35	988230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.626316055281166	18.272016297393353	11.366616508047384	26.73505113927809
2	16.74733078331296	20.581304866555897	35.8157803956689	26.855583954462244
3	18.55319185180273	23.72749058374972	28.843323694516215	28.87599386993133
4	12.655008045847643	15.727396339367466	35.915021247729975	35.702574367054915
5	14.606852311967819	36.36862508426071	33.39181898488053	15.632703618890947
6	34.3815572296908	35.469833017075494	16.47909200030906	13.669517752924651
7	30.232699623473042	30.52429660517355	20.696450158087785	18.546553613265623
8	28.263674730350363	32.45742702318601	19.528297926576936	19.750600319886697
9	27.54029304475004	13.874118760753126	18.65167049135248	39.93391770314435
10	16.16083878645112	26.28380466603557	31.420118415192967	26.13523813232034
11	37.75123075388086	20.946056112156175	22.154969130770187	19.14774400319278
12	24.59275005049559	23.737123625364116	28.43735018628613	23.23277613785417
13	29.41191501166874	19.222270622275975	25.287520654520147	26.07829371153514
14	23.45045470363349	19.758253899111033	24.730548248229002	32.06074314902647
15	25.35541592545635	27.16960676713869	21.94313101840473	25.53184628900024
16	26.06040732295517	25.687895903651984	23.454774205637495	24.796922567755345
17	24.22449729514001	25.663621519150603	24.97511723493467	25.13676395077471
18	25.266592363120456	24.56914488461454	26.02621633526149	24.138046417003505
19	25.777510360721006	24.945732453696156	25.06436666366532	24.212390521917516
20	25.801845583278784	24.30535107208823	24.746122790665982	25.146680553967006
21	26.91773721366569	24.13397026722508	24.22540986598593	24.722882653123303
22	25.964404869964735	24.418327342812713	24.702988608682322	24.914279178540227
23	24.721240025600654	24.038758708967773	25.31441107544649	25.92559018998508
24	24.883860150343004	25.024700250896142	24.87394354715071	25.217496051610137
25	24.889335575418507	24.457142022792368	25.156536319102905	25.496986082686217
26	24.537995799740585	25.277725727440643	25.566341466975885	24.617937005842887
27	25.468878900631985	24.692767815208054	24.701163466990486	25.137189817169475
28	24.534528030526104	24.645131617051202	25.528743548124122	25.29159680429857
29	24.687535742358133	24.784146575912512	25.288494063422455	25.2398236183069
30	24.50051955700161	24.998053182195378	25.595361219876033	24.906066040926977
31	25.188719650935564	24.727019640958126	24.51396476746478	25.570295940641525
32	24.844680442024984	24.724525280645953	24.674881426628087	25.755912850700973
33	24.499241957817325	24.409992529086676	25.247671727581782	25.843093785514213
34	25.231853832919228	24.498998605591748	25.516819289070806	24.752328272418215
35	25.70736408169821	24.46468594178528	25.21421079656484	24.613739179951672
36	24.5488249737788	24.84206440560002	24.9693376195772	25.63977300104398
37	25.529169414518883	24.678045005560598	24.709072414321763	25.083713165598752
38	24.60826375487617	24.379330148663875	25.473076726523203	25.539329369936752
39	25.202347375567925	24.278764841443856	24.896453628016655	25.622434154971565
40	25.46455939862798	24.396729832792687	25.600897483007927	24.537813285571403
41	24.412182699116876	24.625298410666616	26.030596675321892	24.931922214894616
42	25.43998082384463	24.941899656143303	25.313133476262205	24.304986043749864
43	24.500641233114397	23.98710719908889	25.749464016723167	25.762787551073547
44	24.20758431546236	24.44564363013382	25.461274143582678	25.885497910821144
45	24.24037602785896	24.742350831169528	25.726649745575248	25.29062339539626
46	24.16928574818124	24.277395105937437	25.79183868648043	25.761480459400893
47	24.28606540821119	24.150457380507973	26.225278455784117	25.338198755496716
48	24.791082114341474	24.822413713384616	25.382793050833847	25.00371112144006
49	23.849795705806628	24.92376991533776	25.74721300863657	25.47922137021904
50	23.654459097637233	25.218180612481422	25.543968602676753	25.583391687204593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.5
2	19.0
3	60.5
4	102.0
5	131.0
6	160.0
7	183.0
8	206.0
9	268.5
10	331.0
11	475.5
12	620.0
13	1072.0
14	1524.0
15	2143.0
16	2762.0
17	3407.0
18	4052.0
19	4416.5
20	4781.0
21	5848.0
22	6915.0
23	7546.5
24	8178.0
25	9983.0
26	11788.0
27	15007.0
28	18226.0
29	20698.0
30	23170.0
31	25000.5
32	26831.0
33	31094.5
34	35358.0
35	40900.5
36	46443.0
37	55179.5
38	63916.0
39	68282.5
40	72649.0
41	78970.0
42	85291.0
43	86203.0
44	87115.0
45	94304.5
46	101494.0
47	108235.5
48	114977.0
49	118865.0
50	122753.0
51	118581.0
52	114409.0
53	112482.0
54	110555.0
55	112447.0
56	114339.0
57	112168.5
58	109998.0
59	100867.0
60	91736.0
61	80979.0
62	70222.0
63	62199.5
64	54177.0
65	46660.0
66	39143.0
67	34692.0
68	30241.0
69	27535.0
70	24829.0
71	19546.0
72	14263.0
73	12757.0
74	11251.0
75	8549.5
76	5848.0
77	5184.5
78	4521.0
79	4002.5
80	3484.0
81	2773.0
82	2062.0
83	1779.5
84	1497.0
85	1194.0
86	891.0
87	645.5
88	400.0
89	261.0
90	122.0
91	79.0
92	36.0
93	21.5
94	7.0
95	4.5
96	2.0
97	2.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015513704380583413
2	0.0037111214400611303
3	4.258663947611133E-4
4	1.8251416918333427E-4
5	0.0
6	4.258663947611133E-4
7	6.0838056394444754E-5
8	6.0838056394444754E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2167611278888951E-4
47	0.0
48	0.0
49	0.0
50	6.0838056394444754E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1643708.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.762392269127474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15275903968556	46.6318713460174
2	11.160518036564877	12.669954054363355
3	2.870742468664419	4.888506303299194
4	1.1541482644008023	2.620488660826039
5	0.6316643657947725	1.7927390256836249
6	0.41148073404873675	1.4013978502357745
7	0.2724569178097114	1.0825714511606581
8	0.2008884291648451	0.9122326254867011
9	0.15445344806932865	0.7890432485967488
>10	0.8205993997388268	8.568251517373962
>50	0.07568156371918197	3.033571444848602
>100	0.08276820187533968	10.075431635696024
>500	0.009256047453399209	3.568534406417301
>1k	0.002583083010250942	1.9654064299947098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1861	0.11321962295006169	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1836	0.11169867154020058	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1749	0.10640576063388388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0838056394444754E-5
2	0.0	0.0	0.0	0.0	6.0838056394444754E-5
3	0.0	0.0	0.0	0.0	6.0838056394444754E-5
4	0.0	0.0	0.0	0.0	6.0838056394444754E-5
5	0.0	0.0	0.0	0.0	6.0838056394444754E-5
6	0.0	0.0	0.0	0.0	1.2167611278888951E-4
7	0.0	0.0	0.0	0.0	1.2167611278888951E-4
8	0.0	0.0	0.0	0.0	1.2167611278888951E-4
9	0.0	0.0	0.0	6.0838056394444754E-5	1.2167611278888951E-4
10	0.0	0.0	0.0	6.0838056394444754E-5	1.2167611278888951E-4
11	0.0	0.0	0.0	6.0838056394444754E-5	1.2167611278888951E-4
12	0.0	0.0	0.0	1.2167611278888951E-4	3.6502833836666855E-4
13	0.0	0.0	0.0	1.2167611278888951E-4	3.6502833836666855E-4
14	0.0	0.0	0.0	1.2167611278888951E-4	3.6502833836666855E-4
15	0.0	0.0	0.0	1.2167611278888951E-4	4.258663947611133E-4
16	0.0	0.0	0.0	1.2167611278888951E-4	4.258663947611133E-4
17	0.0	0.0	0.0	1.2167611278888951E-4	4.258663947611133E-4
18	0.0	0.0	0.0	2.4335222557777901E-4	4.8670445115555803E-4
19	0.0	0.0	0.0	2.4335222557777901E-4	4.8670445115555803E-4
20	0.0	0.0	0.0	6.692186203388923E-4	4.8670445115555803E-4
21	0.0	0.0	0.0	7.300566767333371E-4	5.475425075500028E-4
22	0.0	0.0	0.0	0.0010342469587055609	5.475425075500028E-4
23	0.0	0.0	0.0	0.0013384372406777846	6.083805639444475E-4
24	0.0	0.0	0.0	0.002311846142988901	6.083805639444475E-4
25	0.0	0.0	0.0	0.002981064763327793	6.692186203388923E-4
26	0.0	0.0	0.0	0.0038327975528500194	6.692186203388923E-4
27	0.0	0.0	0.0	0.006205481752233365	7.300566767333371E-4
28	6.0838056394444754E-5	0.0	0.0	0.017034655790444532	7.300566767333371E-4
29	6.0838056394444754E-5	0.0	0.0	0.0360161293855113	7.300566767333371E-4
30	6.0838056394444754E-5	0.0	0.0	0.07537835187271705	7.300566767333371E-4
31	6.0838056394444754E-5	0.0	0.0	0.16450610449057862	7.300566767333371E-4
32	6.0838056394444754E-5	0.0	0.0	0.27060767484249026	7.300566767333371E-4
33	6.0838056394444754E-5	0.0	0.0	0.38303640305942416	7.300566767333371E-4
34	6.0838056394444754E-5	0.0	0.0	0.5131690056871415	7.908947331277818E-4
35	6.0838056394444754E-5	0.0	0.0	0.6569293329472145	7.908947331277818E-4
36	6.0838056394444754E-5	0.0	0.0	0.857269052654121	7.908947331277818E-4
37	6.0838056394444754E-5	0.0	0.0	1.1328046100645612	7.908947331277818E-4
38	1.2167611278888951E-4	0.0	0.0	1.5002056326306132	7.908947331277818E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	230	0.0	20.086914	35
GTATCAA	2545	0.0	20.056799	1
TAACGGC	245	0.0	18.857105	36
GTAAGAC	110	2.797724E-6	17.999962	3
TATACCG	90	2.2160164E-4	17.111076	5
ATACCGG	130	7.320905E-7	16.923042	6
CGTCTTA	315	0.0	16.76187	15
GTATAAA	225	1.8189894E-12	16.623705	1
TAGGACG	385	0.0	16.571394	4
AACGCAG	3125	0.0	16.191965	6
TAATACT	410	0.0	16.097527	4
CAACGGA	220	1.8189894E-11	15.999966	14
TACCGTC	595	0.0	15.899127	7
ATACCGT	605	0.0	15.636332	6
CGTATTG	655	0.0	15.450351	27
GCATTCG	705	0.0	15.290748	22
ACCGTCG	605	0.0	15.272696	8
GGCGTTA	520	0.0	15.230738	42
CGTCGTA	585	0.0	15.042705	10
TGTAGGA	630	0.0	15.0158415	2
>>END_MODULE