FastQCFastQC Report
Thu 2 Feb 2017
SRR4062490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922894
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG21610.23415473499665182No Hit
GTATCAACGCAGAGTACTTTTTTTT21540.23339625135714395No Hit
TCCATGTACTCTGCGTTGATACCAC16800.182036073481895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16010.17347604383602017No Hit
TATCAACGCAGAGTACTTTTTTTTT14330.15527243648783068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11230.12168244673819528No Hit
CCATAGGGTCTTCTCGTCTTATTAT10660.11550622281648815No Hit
GTATTAGAGGCACTGCCTGCCCAGT10370.11236393345281258No Hit
GAGTACATGGAAGCAGTGGTATCAA9280.10055325963761819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA755.8702426E-815.1928411
GCGACGA456.7494204E-414.78043713
CGACGAC456.7494204E-414.78043714
CGAACGA456.752198E-414.77963416
GTACGAA400.00526495814.25488619
TTTTCGG551.9567015E-413.8199216
AACACTA551.9621133E-413.8154215
AGAATTT1450.013.7625917
GAATTTC1500.013.30383818
ATGTACG500.001495484513.30383717
GACGACC500.001496708413.30239315
ACGGTAT500.00149854613.3002289
ACTAGAC500.001503455513.2944573
GTACTAA500.001504070113.2937361
GATATAC1450.013.0972771
CAAGACG1102.750312E-912.9512554
CGGTCCA1403.6379788E-1212.89377810
GGCGACG604.0848853E-412.66825912
TTGAACC604.0979957E-412.6634474
CCCTTAA751.4842102E-512.6607011
Produced by FastQC (version 0.11.5)