##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062490_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922894
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.053065682516085	32.0	32.0	32.0	32.0	32.0
2	30.59698838653193	32.0	32.0	32.0	32.0	32.0
3	30.60429908526873	32.0	32.0	32.0	32.0	32.0
4	30.684918311312025	32.0	32.0	32.0	32.0	32.0
5	30.4233086356613	32.0	32.0	32.0	32.0	32.0
6	34.06216856973823	36.0	36.0	36.0	32.0	36.0
7	33.91774786703565	36.0	36.0	36.0	32.0	36.0
8	33.8957648440666	36.0	36.0	36.0	32.0	36.0
9	34.130402841496426	36.0	36.0	36.0	32.0	36.0
10	33.69727075915544	36.0	36.0	36.0	27.0	36.0
11	34.132769310451685	36.0	36.0	36.0	32.0	36.0
12	33.83966631054054	36.0	36.0	36.0	32.0	36.0
13	34.002622186296584	36.0	36.0	36.0	32.0	36.0
14	33.87691327498066	36.0	36.0	36.0	32.0	36.0
15	33.80014822937412	36.0	36.0	36.0	32.0	36.0
16	33.805881282140746	36.0	36.0	36.0	32.0	36.0
17	33.72370933173257	36.0	36.0	36.0	27.0	36.0
18	33.74302032519444	36.0	36.0	36.0	32.0	36.0
19	33.73689177738722	36.0	36.0	36.0	32.0	36.0
20	33.7143756487744	36.0	36.0	36.0	27.0	36.0
21	33.70427481379227	36.0	36.0	36.0	27.0	36.0
22	33.64583148227207	36.0	36.0	36.0	27.0	36.0
23	33.60439660459381	36.0	36.0	36.0	27.0	36.0
24	33.57860274311026	36.0	36.0	36.0	27.0	36.0
25	33.09361205078807	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	28.0
5	80.0
6	154.0
7	57.0
8	145.0
9	169.0
10	118.0
11	33.0
12	51.0
13	45.0
14	140.0
15	190.0
16	334.0
17	398.0
18	601.0
19	843.0
20	1523.0
21	2240.0
22	3495.0
23	5116.0
24	7267.0
25	10028.0
26	13802.0
27	17640.0
28	23299.0
29	30604.0
30	38539.0
31	50969.0
32	71453.0
33	99385.0
34	205695.0
35	338450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.390107812125805	17.90457180196552	10.934765482347917	25.77055490356076
2	16.445162024370024	20.332338232983513	37.50426665077396	25.718233091872506
3	19.136230552346937	24.29897427784436	28.605143564259876	27.959651605548824
4	12.059212379982228	15.622791998092719	36.9740349812523	35.34396064067275
5	13.794482640511003	37.167223619874285	34.53080982766787	14.507483911946837
6	33.14539872778281	36.310223266745005	17.269676222026614	13.274701783445567
7	29.379057895901013	31.31654732422832	21.114395256951283	18.189999522919386
8	27.579583917612087	33.35263757798969	20.017851003766495	19.049927500631725
9	26.97277657266811	14.744685466377442	19.226138828633406	39.05639913232104
10	15.35582515266542	27.037699598714237	32.82016344339853	24.786311805221814
11	36.36637806183831	21.762525344682253	22.930902992109925	18.940193601369508
12	23.95749925679033	24.7706899806657	29.464954137996134	21.806856624547834
13	28.707690672013005	20.18349818226792	25.91019503055591	25.198616115163176
14	22.649042258655257	20.711319313654865	25.909680695024257	30.729957732665618
15	24.665295522737775	27.729216430296205	23.31855882359322	24.2869292233728
16	25.12127602336136	26.52028932684214	24.536808032421266	23.82162661737523
17	23.00723183308417	26.536574675782582	26.296923921463115	24.15926956967013
18	23.759017216930722	25.569241485110812	27.443694267930553	23.228047030027916
19	24.603984029164135	26.189458380348928	26.090942626508117	23.11561496397882
20	24.22415233367254	25.621253116300668	26.434366205739853	23.720228344286944
21	25.47990387275617	25.226349280401866	25.215933687391168	24.077813159450795
22	24.315372870154437	25.900202607307538	25.669705595412633	24.1147189271254
23	23.61765478208219	25.539016434711463	26.24692649410907	24.596402289097274
24	23.620366773792448	26.521551378059975	26.04089586375857	23.817185984389003
25	24.0469316390378	25.69728572033057	26.242702193058122	24.013080447573508
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	155.0
1	155.0
2	349.5
3	544.0
4	544.0
5	544.0
6	1865.5
7	3187.0
8	3187.0
9	3187.0
10	3238.0
11	3289.0
12	3289.0
13	3289.0
14	3516.0
15	3743.0
16	3743.0
17	3743.0
18	6123.0
19	8503.0
20	8503.0
21	8503.0
22	13445.0
23	18387.0
24	18387.0
25	18387.0
26	26607.0
27	34827.0
28	34827.0
29	34827.0
30	42504.5
31	50182.0
32	50182.0
33	50182.0
34	58519.5
35	66857.0
36	66857.0
37	66857.0
38	72908.0
39	78959.0
40	78959.0
41	78959.0
42	86444.5
43	93930.0
44	93930.0
45	93930.0
46	108938.0
47	123946.0
48	123946.0
49	123946.0
50	121062.5
51	118179.0
52	118179.0
53	118179.0
54	108791.0
55	99403.0
56	99403.0
57	99403.0
58	89321.5
59	79240.0
60	79240.0
61	79240.0
62	69405.5
63	59571.0
64	59571.0
65	59571.0
66	48996.0
67	38421.0
68	38421.0
69	38421.0
70	29093.0
71	19765.0
72	19765.0
73	19765.0
74	15162.0
75	10559.0
76	10559.0
77	10559.0
78	8509.0
79	6459.0
80	6459.0
81	6459.0
82	4539.5
83	2620.0
84	2620.0
85	2620.0
86	1909.5
87	1199.0
88	1199.0
89	1199.0
90	830.0
91	461.0
92	461.0
93	461.0
94	265.5
95	70.0
96	70.0
97	70.0
98	254.0
99	438.0
100	438.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009210158479738734
2	0.0042258374201154195
3	0.0034673537806075238
4	0.012352447843414304
5	0.03413176377785531
6	0.0444254703140339
7	0.0669632698879828
8	0.08852587621113583
9	0.0968691962457227
10	0.11994876984789153
11	0.11951535062531557
12	0.1317594436630859
13	0.12363283323978702
14	0.12374118804543101
15	0.11225557864716859
16	0.11160544981330467
17	0.1230910592115671
18	0.12840044468812237
19	0.13154273405179795
20	0.1222242207664152
21	0.12991741196713816
22	0.15245521154108704
23	0.14172808578233254
24	0.1339265397759656
25	0.13143437924615395
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	922894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38629594332254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94711803852958	44.83361035441062
2	12.106967321926247	13.411201501366843
3	3.19926782608497	5.315867838524769
4	1.2236999928175472	2.7110483979213735
5	0.5997738478363535	1.6609625917664788
6	0.367568748854865	1.221496290215541
7	0.24023497747642508	0.9314007890912692
8	0.1716057754896779	0.7603686613483732
9	0.1318476958775186	0.657229995298569
>10	0.7817962420629699	8.444830241063492
>50	0.11339436727329645	4.45998586419128
>100	0.10789721208778877	11.713881983427818
>500	0.00725853969475319	2.54654804357621
>1k	0.001569413988054744	1.3315674477973483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2161	0.23415473499665182	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2154	0.23339625135714395	No Hit
TCCATGTACTCTGCGTTGATACCAC	1680	0.182036073481895	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1601	0.17347604383602017	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1433	0.15527243648783068	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1123	0.12168244673819528	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1066	0.11550622281648815	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1037	0.11236393345281258	No Hit
GAGTACATGGAAGCAGTGGTATCAA	928	0.10055325963761819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0835480564398512E-4
2	0.0	0.0	0.0	0.0	1.0835480564398512E-4
3	0.0	0.0	0.0	0.0	1.0835480564398512E-4
4	0.0	0.0	0.0	0.0	1.0835480564398512E-4
5	0.0	1.0835480564398512E-4	0.0	0.0	1.0835480564398512E-4
6	0.0	1.0835480564398512E-4	0.0	0.0	2.1670961128797024E-4
7	0.0	1.0835480564398512E-4	0.0	0.0	2.1670961128797024E-4
8	0.0	1.0835480564398512E-4	0.0	0.0	2.1670961128797024E-4
9	0.0	1.0835480564398512E-4	0.0	0.0	2.1670961128797024E-4
10	0.0	1.0835480564398512E-4	0.0	0.0	3.2506441693195534E-4
11	0.0	1.0835480564398512E-4	0.0	0.0	3.2506441693195534E-4
12	0.0	1.0835480564398512E-4	0.0	0.0	6.501288338639107E-4
13	0.0	3.2506441693195534E-4	0.0	0.0	6.501288338639107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	75	5.8702426E-8	15.192841	1
GCGACGA	45	6.7494204E-4	14.780437	13
CGACGAC	45	6.7494204E-4	14.780437	14
CGAACGA	45	6.752198E-4	14.779634	16
GTACGAA	40	0.005264958	14.254886	19
TTTTCGG	55	1.9567015E-4	13.81992	16
AACACTA	55	1.9621133E-4	13.815421	5
AGAATTT	145	0.0	13.76259	17
GAATTTC	150	0.0	13.303838	18
ATGTACG	50	0.0014954845	13.303837	17
GACGACC	50	0.0014967084	13.302393	15
ACGGTAT	50	0.001498546	13.300228	9
ACTAGAC	50	0.0015034555	13.294457	3
GTACTAA	50	0.0015040701	13.293736	1
GATATAC	145	0.0	13.097277	1
CAAGACG	110	2.750312E-9	12.951255	4
CGGTCCA	140	3.6379788E-12	12.893778	10
GGCGACG	60	4.0848853E-4	12.668259	12
TTGAACC	60	4.0979957E-4	12.663447	4
CCCTTAA	75	1.4842102E-5	12.660701	1
>>END_MODULE