FastQCFastQC Report
Thu 2 Feb 2017
SRR4062490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922894
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT10400.11268899786974453No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG10150.10998012772864489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAT450.001397963624.44444311
GCTAGAC956.862683E-720.8421063
CGCGGGA2050.020.39024544
AAACGCT2250.019.55555529
GTCGATG1051.79175E-618.85714312
TATACTG951.596443E-518.5263165
GTAAACG2300.018.17391427
TAACGGC2750.017.636
GGTCGGA2400.017.41666838
GTATATC2150.017.3953483
ACGCTTC2800.017.28571531
CGTTTAT2550.017.254931
AGACCGT1154.2706724E-617.2173926
TAATACC902.2146571E-417.111114
TAAACGC2450.017.06122428
GATTTCG1307.3121737E-716.92307741
CGCAATA1954.9112714E-1116.92307736
ATACCGT1702.4647306E-916.823536
ATAATAC1053.7727834E-516.7619063
GATTAAT2500.016.7238
Produced by FastQC (version 0.11.5)