##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	922894
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23825163019805	32.0	32.0	32.0	32.0	32.0
2	31.38409828214291	32.0	32.0	32.0	32.0	32.0
3	31.48945382676667	32.0	32.0	32.0	32.0	32.0
4	31.596408688321734	32.0	32.0	32.0	32.0	32.0
5	31.540453183139125	32.0	32.0	32.0	32.0	32.0
6	35.12000294725071	36.0	36.0	36.0	36.0	36.0
7	35.12871359007644	36.0	36.0	36.0	36.0	36.0
8	35.06985634319868	36.0	36.0	36.0	36.0	36.0
9	35.2130970620678	36.0	36.0	36.0	36.0	36.0
10	35.087539847479775	36.0	36.0	36.0	36.0	36.0
11	35.21219988427707	36.0	36.0	36.0	36.0	36.0
12	35.122388920070996	36.0	36.0	36.0	36.0	36.0
13	35.171271023541166	36.0	36.0	36.0	36.0	36.0
14	35.14084391056828	36.0	36.0	36.0	36.0	36.0
15	35.08899288542346	36.0	36.0	36.0	36.0	36.0
16	35.10680424837522	36.0	36.0	36.0	36.0	36.0
17	35.08825390564897	36.0	36.0	36.0	36.0	36.0
18	35.09086959065721	36.0	36.0	36.0	36.0	36.0
19	35.073004050302636	36.0	36.0	36.0	36.0	36.0
20	35.06583746345734	36.0	36.0	36.0	36.0	36.0
21	35.05772277206266	36.0	36.0	36.0	36.0	36.0
22	35.03840636086051	36.0	36.0	36.0	36.0	36.0
23	35.006858859197266	36.0	36.0	36.0	36.0	36.0
24	34.976864081898896	36.0	36.0	36.0	36.0	36.0
25	34.954442221966985	36.0	36.0	36.0	32.0	36.0
26	34.90915858159225	36.0	36.0	36.0	32.0	36.0
27	34.888310033438295	36.0	36.0	36.0	32.0	36.0
28	34.8790066898257	36.0	36.0	36.0	32.0	36.0
29	34.85482298075402	36.0	36.0	36.0	32.0	36.0
30	34.82703755794273	36.0	36.0	36.0	32.0	36.0
31	34.83117021022999	36.0	36.0	36.0	32.0	36.0
32	34.78895301085498	36.0	36.0	36.0	32.0	36.0
33	34.767669959930394	36.0	36.0	36.0	32.0	36.0
34	34.75681064130875	36.0	36.0	36.0	32.0	36.0
35	34.70737376123368	36.0	36.0	36.0	32.0	36.0
36	34.68090809995514	36.0	36.0	36.0	32.0	36.0
37	34.656011416262324	36.0	36.0	36.0	32.0	36.0
38	34.62649014946462	36.0	36.0	36.0	32.0	36.0
39	34.60222734138482	36.0	36.0	36.0	32.0	36.0
40	34.5855840432379	36.0	36.0	36.0	32.0	36.0
41	34.560753455976524	36.0	36.0	36.0	32.0	36.0
42	34.49881134778209	36.0	36.0	36.0	32.0	36.0
43	34.50474810758332	36.0	36.0	36.0	32.0	36.0
44	34.46698645781639	36.0	36.0	36.0	32.0	36.0
45	34.40225312982856	36.0	36.0	36.0	32.0	36.0
46	34.40857671628594	36.0	36.0	36.0	32.0	36.0
47	34.361817283458336	36.0	36.0	36.0	32.0	36.0
48	34.328777736121374	36.0	36.0	36.0	32.0	36.0
49	34.3256777051319	36.0	36.0	36.0	32.0	36.0
50	33.801984843329784	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	13.0
21	19.0
22	81.0
23	227.0
24	601.0
25	1445.0
26	3081.0
27	5959.0
28	9746.0
29	15037.0
30	21383.0
31	30498.0
32	44606.0
33	71981.0
34	157618.0
35	560596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87760078287642	18.472047171988976	11.463547587594054	26.186804457540553
2	15.834443121247354	20.730043593263904	37.29808451870241	26.13742876678633
3	18.405118703204064	24.673471377954034	29.069553251200038	27.851856667641865
4	11.874845594067345	16.16061250937271	37.14963397667332	34.814907919886615
5	13.598744817931422	37.41513109847935	34.43851623263343	14.5476078509558
6	33.322028278529096	36.52890753079183	16.99799868023418	13.151065510444896
7	29.343564916447612	31.35376327075482	21.05583089715612	18.24684091564145
8	27.420050406655587	33.72705857877503	19.797831603629454	19.055059410939933
9	27.041350360929854	14.506974798839304	19.07943924220983	39.37223559802101
10	15.256464989478749	27.117740498908866	32.91526437489029	24.710530136722095
11	36.56357068092327	21.741933526493835	22.857337895793016	18.83715789678988
12	23.907837736511453	24.721257262480847	29.3797554215327	21.991149579475
13	28.759424159220888	20.147492561442593	25.951842790179587	25.141240489156935
14	22.63076799719144	20.616668869881046	25.918902929263815	30.833660203663694
15	24.78280279208663	27.729728441186097	23.225094106148703	24.26237466057857
16	25.108517337852447	26.538042288713548	24.49187013893253	23.86157023450147
17	23.089108825065498	26.429904192680848	26.175595463834416	24.305391518419235
18	23.7520235259954	25.47313125884446	27.58269096992721	23.192154245232928
19	24.595565687933828	26.25642814884483	26.123585157125305	23.02442100609604
20	24.35881043760172	25.484075094214504	26.437922448298508	23.719192019885273
21	25.489817898913635	25.133222233539282	25.2544712610549	24.122488606492187
22	24.295314521494344	25.86006626979913	25.721155408963543	24.123463799742982
23	23.647136074132025	25.302689149566472	26.239199734747437	24.810975041554066
24	23.622214468833906	26.48332311186333	25.969721333110847	23.924741086191915
25	24.08998216479899	25.496319187252276	26.288826235732383	24.124872412216355
26	23.227911331095445	26.01382173900795	26.92562742850208	23.832639501394524
27	24.26584201435918	26.223379933123415	25.817374476375406	23.693403576142007
28	23.471492934183125	25.558839910108855	26.631335776373017	24.338331379335003
29	23.55861019792089	25.97903984639623	26.55657096047867	23.905778995204216
30	24.05541698179856	25.708477896703197	26.774472474628723	23.46163264686952
31	23.735553595537514	25.629162178971796	26.12618567246076	24.509098553029926
32	23.19291272887244	26.06399001402111	26.20604316422038	24.537054092886073
33	23.421324659169958	25.606516024592207	26.468478503490108	24.503680812747728
34	23.65818826430771	25.7732740704783	27.133885364949823	23.43465230026417
35	24.609110038639322	25.776199650230687	26.162917951574073	23.45177235955592
36	23.494355798174006	25.8417543076453	26.371934371661315	24.29195552251938
37	24.540738156277968	25.771757103199285	25.72494782716108	23.962556913361666
38	23.56771200159498	25.954768369931973	26.294135621208937	24.183384007264106
39	23.70608108840235	25.970804881167286	25.678571970345455	24.644542060084905
40	24.2979150368298	25.886829906793196	26.453633895116884	23.361621161260125
41	23.450038682665614	25.839803921143705	26.863431770062434	23.846725626128247
42	24.20635522606063	26.345929218306765	26.0065619670298	23.44115358860281
43	23.44180371743667	25.739684080728665	26.242125314499827	24.576386887334838
44	23.443429039521334	25.839803921143705	26.480072467694015	24.236694571640946
45	23.486987671390214	26.10993245161416	26.53999267521514	23.863087201780488
46	23.648219622188464	25.707611058258045	26.137346217442094	24.5068231021114
47	23.900036190505087	25.64411514215067	26.430771031126	24.025077636218246
48	23.99831399922418	26.599696173124975	25.59037115855125	23.811618669099595
49	23.09615188743236	25.942849341311135	26.590811079062167	24.370187692194335
50	22.832007610849793	26.756406213938128	25.87017129829785	24.541414876914224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	42.0
2	33.0
3	132.0
4	231.0
5	224.5
6	218.0
7	199.5
8	181.0
9	261.0
10	341.0
11	480.0
12	619.0
13	1114.0
14	1609.0
15	2080.5
16	2552.0
17	3038.0
18	3524.0
19	3938.5
20	4353.0
21	5323.0
22	6293.0
23	6431.0
24	6569.0
25	8178.5
26	9788.0
27	12644.5
28	15501.0
29	17096.5
30	18692.0
31	19118.5
32	19545.0
33	22286.0
34	25027.0
35	28750.0
36	32473.0
37	38143.0
38	43813.0
39	45537.0
40	47261.0
41	50615.5
42	53970.0
43	53146.0
44	52322.0
45	56391.0
46	60460.0
47	63363.0
48	66266.0
49	65102.5
50	63939.0
51	61677.5
52	59416.0
53	58064.5
54	56713.0
55	56885.0
56	57057.0
57	55513.5
58	53970.0
59	48673.5
60	43377.0
61	38627.5
62	33878.0
63	29149.5
64	24421.0
65	21039.0
66	17657.0
67	15340.0
68	13023.0
69	11768.5
70	10514.0
71	8175.0
72	5836.0
73	5169.0
74	4502.0
75	3395.0
76	2288.0
77	2019.5
78	1751.0
79	1466.5
80	1182.0
81	955.5
82	729.0
83	601.0
84	473.0
85	371.5
86	270.0
87	204.5
88	139.0
89	92.5
90	46.0
91	29.5
92	13.0
93	9.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015494737207089873
2	0.00487596625397933
3	4.3341922257594047E-4
4	2.1670961128797024E-4
5	0.0
6	3.2506441693195534E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	1.0835480564398512E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	922894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.60068857787382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24891229370809	47.61242206614572
2	12.10045648562727	14.181901643287143
3	3.136895304122542	5.514726744548396
4	1.1751714590431586	2.7546342678797497
5	0.5923628762063644	1.7356436216831388
6	0.35353181596559186	1.2430324709861909
7	0.22100521192856634	0.9065740318819046
8	0.1568400634003417	0.7352748569485928
9	0.1203525792330287	0.6347469613660738
>10	0.6950227723418084	7.81069702163113
>50	0.10130796849214137	4.188319917347718
>100	0.0916356000185634	10.271449102875522
>500	0.006133823060314254	2.1867403073822045
>1k	3.717468521402578E-4	0.2238369860365825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1040	0.11268899786974453	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1015	0.10998012772864489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.1670961128797024E-4	0.0
12	0.0	0.0	0.0	2.1670961128797024E-4	2.1670961128797024E-4
13	0.0	0.0	0.0	2.1670961128797024E-4	2.1670961128797024E-4
14	0.0	0.0	0.0	2.1670961128797024E-4	2.1670961128797024E-4
15	0.0	0.0	0.0	3.2506441693195534E-4	4.3341922257594047E-4
16	1.0835480564398512E-4	0.0	0.0	3.2506441693195534E-4	4.3341922257594047E-4
17	1.0835480564398512E-4	0.0	0.0	4.3341922257594047E-4	4.3341922257594047E-4
18	1.0835480564398512E-4	0.0	0.0	4.3341922257594047E-4	5.417740282199256E-4
19	1.0835480564398512E-4	0.0	0.0	4.3341922257594047E-4	5.417740282199256E-4
20	1.0835480564398512E-4	0.0	0.0	7.584836395078959E-4	0.0010835480564398512
21	1.0835480564398512E-4	0.0	0.0	0.0013002576677278214	0.0010835480564398512
22	1.0835480564398512E-4	0.0	0.0	0.002492160529811658	0.0013002576677278214
23	1.0835480564398512E-4	0.0	0.0	0.00465925664269136	0.0013002576677278214
24	1.0835480564398512E-4	0.0	0.0	0.0072597719781470026	0.0013002576677278214
25	1.0835480564398512E-4	0.0	0.0	0.009860287313602646	0.0013002576677278214
26	1.0835480564398512E-4	0.0	0.0	0.012352447843414303	0.0013002576677278214
27	1.0835480564398512E-4	0.0	0.0	0.014844608373225961	0.0014086124733718064
28	1.0835480564398512E-4	0.0	0.0	0.021670961128797024	0.0014086124733718064
29	1.0835480564398512E-4	0.0	0.0	0.03532366663993915	0.0014086124733718064
30	1.0835480564398512E-4	0.0	0.0	0.058836659464683916	0.0014086124733718064
31	1.0835480564398512E-4	0.0	0.0	0.1056459355028855	0.0015169672790157917
32	1.0835480564398512E-4	0.0	0.0	0.1645909497732134	0.0015169672790157917
33	1.0835480564398512E-4	0.0	0.0	0.22461951209998116	0.0015169672790157917
34	1.0835480564398512E-4	0.0	0.0	0.2887655570412203	0.0017336768903037619
35	1.0835480564398512E-4	0.0	0.0	0.3677562103556855	0.0017336768903037619
36	1.0835480564398512E-4	0.0	0.0	0.47177682377391117	0.0017336768903037619
37	1.0835480564398512E-4	0.0	0.0	0.6292163563746216	0.001842031695947747
38	1.0835480564398512E-4	0.0	0.0	0.8281557795369783	0.001950386501591732
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAT	45	0.0013979636	24.444443	11
GCTAGAC	95	6.862683E-7	20.842106	3
CGCGGGA	205	0.0	20.390245	44
AAACGCT	225	0.0	19.555555	29
GTCGATG	105	1.79175E-6	18.857143	12
TATACTG	95	1.596443E-5	18.526316	5
GTAAACG	230	0.0	18.173914	27
TAACGGC	275	0.0	17.6	36
GGTCGGA	240	0.0	17.416668	38
GTATATC	215	0.0	17.395348	3
ACGCTTC	280	0.0	17.285715	31
CGTTTAT	255	0.0	17.2549	31
AGACCGT	115	4.2706724E-6	17.217392	6
TAATACC	90	2.2146571E-4	17.11111	4
TAAACGC	245	0.0	17.061224	28
GATTTCG	130	7.3121737E-7	16.923077	41
CGCAATA	195	4.9112714E-11	16.923077	36
ATACCGT	170	2.4647306E-9	16.82353	6
ATAATAC	105	3.7727834E-5	16.761906	3
GATTAAT	250	0.0	16.72	38
>>END_MODULE