FastQCFastQC Report
Thu 2 Feb 2017
SRR4062489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233251
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43170.19330563380470894No Hit
GTACATGGAAGCAGTGGTATCAACG35710.1599014172612035No Hit
GCGCAAGACGGACCAGAGCGAAAGC34410.15408030713968113No Hit
GTCTTGCGCCGGTCCAAGAATTTCA32240.14436353101375526No Hit
TCCATGTACTCTGCGTTGATACCAC31240.1398857539971996No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31200.13970664291653737No Hit
GAATAGGACCGCGGTTCTATTTTGT30900.13836330981157066No Hit
TATCAACGCAGAGTACTTTTTTTTT30450.1363483101541206No Hit
GAATAACGCCGCCGCATCGCCAGTC29640.1327213107707105No Hit
GATTAAGAGGGACGGCCGGGGGCAT28410.12721364504034702No Hit
GAACTACGACGGTATCTGATCGTCT28240.12645242294753253No Hit
GTATCTGATCGTCTTCGAACCTCCG28190.12622853409670476No Hit
GTCCTATTCCATTATTCCTAGCTGC26120.1169595356724345No Hit
CTATTGGAGCTGGAATTACCGCGGC25120.11248175865587881No Hit
GGGTAGGCACACGCTGAGCCAGTCA24740.11078020338958765No Hit
ATCAGATACCGTCGTAGTTCCGACC24490.10966075913544872No Hit
TCGTAGTTCCGACCATAAACGATGC23210.10392920455425746No Hit
CTCTTAATCATGGCCTCAGTTCCGA22460.1005708717918407No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22460.1005708717918407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC1001.8189894E-1216.1369921
CGTTATT2150.013.6897082
GCGTTAT2150.013.2451371
AAGACGG6250.013.0714295
CAAGACG6250.013.0699634
CGCATCG5600.012.894896513
GTTCTAA1207.512426E-1012.6564651
CGCCAGT5800.012.45136318
ATCGCCA5950.012.13691716
TTAGTGC550.00307739512.0865863
GTTATTC2600.012.0533833
CGCAAGA6300.011.9057712
CGAACGA2250.011.82462316
TCGCGTA2900.011.7941759
AATCGTA658.015177E-411.69415813
CATCGCC6350.011.67113715
CGGTCCA5700.011.667726510
CGACCAT3550.011.50808810
CTTATGC752.081486E-411.3959253
GTATAGG1251.8550054E-811.3908191
Produced by FastQC (version 0.11.5)