##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062489_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2233251
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249337848723677	32.0	32.0	32.0	32.0	32.0
2	30.923249558603132	32.0	32.0	32.0	32.0	32.0
3	30.904092732970902	32.0	32.0	32.0	32.0	32.0
4	30.978826383599515	32.0	32.0	32.0	32.0	32.0
5	30.782942445788674	32.0	32.0	32.0	32.0	32.0
6	34.44470684217762	36.0	36.0	36.0	32.0	36.0
7	34.34002626663998	36.0	36.0	36.0	32.0	36.0
8	34.345413032390894	36.0	36.0	36.0	32.0	36.0
9	34.490402556631565	36.0	36.0	36.0	32.0	36.0
10	34.17787700531647	36.0	36.0	36.0	32.0	36.0
11	34.46926431466951	36.0	36.0	36.0	32.0	36.0
12	34.249019031000095	36.0	36.0	36.0	32.0	36.0
13	34.3810337485576	36.0	36.0	36.0	32.0	36.0
14	34.287673888873215	36.0	36.0	36.0	32.0	36.0
15	34.22423766965738	36.0	36.0	36.0	32.0	36.0
16	34.22371242641333	36.0	36.0	36.0	32.0	36.0
17	34.14357006892642	36.0	36.0	36.0	32.0	36.0
18	34.15958394287073	36.0	36.0	36.0	32.0	36.0
19	34.14211031361902	36.0	36.0	36.0	32.0	36.0
20	34.147903661523046	36.0	36.0	36.0	32.0	36.0
21	34.117228202293425	36.0	36.0	36.0	32.0	36.0
22	34.07771450678853	36.0	36.0	36.0	32.0	36.0
23	34.04441551800492	36.0	36.0	36.0	32.0	36.0
24	34.020993161986716	36.0	36.0	36.0	32.0	36.0
25	33.57470566452226	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	87.0
5	239.0
6	365.0
7	78.0
8	346.0
9	401.0
10	258.0
11	81.0
12	168.0
13	136.0
14	320.0
15	382.0
16	605.0
17	882.0
18	1151.0
19	1554.0
20	2260.0
21	3450.0
22	5354.0
23	7837.0
24	11466.0
25	16430.0
26	23295.0
27	30811.0
28	42651.0
29	58327.0
30	76090.0
31	105782.0
32	153349.0
33	225580.0
34	510850.0
35	952653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.211415131896835	17.514490134885992	11.287057151340015	25.987037581877154
2	17.346161079715923	19.71878666296492	36.03717568668894	26.897876570630224
3	19.118207469058046	23.09750471646785	28.358543803705377	29.425744010768728
4	12.997556080933714	14.901994680434182	35.02435633835807	37.07609290027404
5	15.221951517773993	35.76438768759124	33.039082226812106	15.974578567822661
6	35.122199250520225	34.522705026173554	16.371362077577654	13.983733645728568
7	30.81513152529553	30.145474065504107	20.44523147430388	18.59416293489648
8	28.66568606096786	31.919253305510665	19.48205375045826	19.933006883063218
9	27.18396427900706	14.325957646312148	18.822412036561843	39.667666038118945
10	16.470598256342804	26.01924415818352	30.6239714085768	26.886186176896874
11	37.89544707683548	20.98074685783071	21.722000676964598	19.401805388369212
12	24.85806498475953	23.575784913413138	27.88867237067122	23.677477731156117
13	29.2436817310434	18.791077938916377	25.327637192470263	26.63760313756996
14	23.731397953881405	19.70112088634353	24.3354866940421	32.23199446573296
15	25.46922574837629	26.80777658632104	22.028012781660973	25.6949848836417
16	26.43537130745699	25.463439475826554	23.25741736270751	24.843771854008946
17	24.41205691310601	25.639008145302732	24.9016807084369	25.04725423315436
18	25.55878082878462	24.20770819377824	25.933483447533114	24.30002752990402
19	25.717165521533346	25.022463265163374	24.995964682904784	24.264406530398492
20	25.751876496871084	23.969529510608897	24.73169524489282	25.5468987476272
21	27.107999408148714	24.025754498293946	24.021763985849372	24.844482107707965
22	25.96789889812228	24.101344048649448	24.597884143634268	25.332872909594006
23	24.39808995883161	23.82318532412541	25.271096614560097	26.507628102482883
24	24.767967887894603	25.04444694548036	24.860740377376427	25.326844789248614
25	25.07465403571198	24.25978169635491	24.805002761975135	25.860561505957975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	623.0
3	1024.0
4	1024.0
5	1024.0
6	2621.5
7	4219.0
8	4219.0
9	4219.0
10	4567.5
11	4916.0
12	4916.0
13	4916.0
14	5536.5
15	6157.0
16	6157.0
17	6157.0
18	9837.0
19	13517.0
20	13517.0
21	13517.0
22	21926.5
23	30336.0
24	30336.0
25	30336.0
26	45466.0
27	60596.0
28	60596.0
29	60596.0
30	73860.0
31	87124.0
32	87124.0
33	87124.0
34	109853.0
35	132582.0
36	132582.0
37	132582.0
38	150708.5
39	168835.0
40	168835.0
41	168835.0
42	195010.0
43	221185.0
44	221185.0
45	221185.0
46	256776.5
47	292368.0
48	292368.0
49	292368.0
50	295248.5
51	298129.0
52	298129.0
53	298129.0
54	277682.0
55	257235.0
56	257235.0
57	257235.0
58	239974.0
59	222713.0
60	222713.0
61	222713.0
62	198513.0
63	174313.0
64	174313.0
65	174313.0
66	145947.5
67	117582.0
68	117582.0
69	117582.0
70	89543.0
71	61504.0
72	61504.0
73	61504.0
74	48770.0
75	36036.0
76	36036.0
77	36036.0
78	30433.5
79	24831.0
80	24831.0
81	24831.0
82	17397.5
83	9964.0
84	9964.0
85	9964.0
86	7445.5
87	4927.0
88	4927.0
89	4927.0
90	3312.5
91	1698.0
92	1698.0
93	1698.0
94	967.5
95	237.0
96	237.0
97	237.0
98	619.0
99	1001.0
100	1001.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008059998629800233
2	0.003179221681754537
3	0.005283776879535709
4	0.01620955279993158
5	0.036001327213107705
6	0.0463897698915169
7	0.06774876626048751
8	0.08852565161730588
9	0.09770509450124504
10	0.11875064647905677
11	0.12116864606799684
12	0.13455719934749832
13	0.12246720140279797
14	0.12466131214091028
15	0.11440720277299775
16	0.11369075845034884
17	0.12372097896743356
18	0.13204964421822715
19	0.1317809775972338
20	0.1238105345077647
21	0.13258697746021383
22	0.15318475173636997
23	0.14069175386017962
24	0.1371990877872662
25	0.13267653300054497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2233251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.55306655535072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75579117865338	37.450793795378075
2	12.635684135916737	12.017310573752756
3	3.5837386252588193	5.112532840917411
4	1.6015297060911173	3.046305948164887
5	0.8503411694085181	2.0218165111819006
6	0.5182641858465258	1.47870307936887
7	0.35409054968676257	1.178666403311274
8	0.2500601316349165	0.9512900865979959
9	0.2004280424528742	0.8577871238088168
>10	1.0352153997882108	8.896656734229294
>50	0.08727974725222137	2.9148857979190805
>100	0.099386754106921	10.261711990729996
>500	0.018385026458592097	6.046636669514975
>1k	0.009805347444582453	7.764902445124735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4317	0.19330563380470894	No Hit
GTACATGGAAGCAGTGGTATCAACG	3571	0.1599014172612035	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3441	0.15408030713968113	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3224	0.14436353101375526	No Hit
TCCATGTACTCTGCGTTGATACCAC	3124	0.1398857539971996	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3120	0.13970664291653737	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3090	0.13836330981157066	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3045	0.1363483101541206	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2964	0.1327213107707105	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2841	0.12721364504034702	No Hit
GAACTACGACGGTATCTGATCGTCT	2824	0.12645242294753253	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2819	0.12622853409670476	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2612	0.1169595356724345	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2512	0.11248175865587881	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2474	0.11078020338958765	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2449	0.10966075913544872	No Hit
TCGTAGTTCCGACCATAAACGATGC	2321	0.10392920455425746	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2246	0.1005708717918407	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2246	0.1005708717918407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.4777770165556846E-5
3	0.0	0.0	0.0	0.0	4.4777770165556846E-5
4	0.0	0.0	0.0	0.0	4.4777770165556846E-5
5	0.0	0.0	0.0	4.4777770165556846E-5	4.4777770165556846E-5
6	0.0	0.0	0.0	4.4777770165556846E-5	1.3433331049667055E-4
7	0.0	0.0	0.0	4.4777770165556846E-5	1.3433331049667055E-4
8	0.0	0.0	0.0	4.4777770165556846E-5	1.3433331049667055E-4
9	0.0	0.0	0.0	4.4777770165556846E-5	2.2388885082778425E-4
10	0.0	0.0	0.0	4.4777770165556846E-5	2.2388885082778425E-4
11	0.0	0.0	0.0	4.4777770165556846E-5	2.686666209933411E-4
12	0.0	0.0	0.0	4.4777770165556846E-5	4.0299993149001167E-4
13	0.0	0.0	0.0	4.4777770165556846E-5	4.0299993149001167E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	100	1.8189894E-12	16.136992	1
CGTTATT	215	0.0	13.689708	2
GCGTTAT	215	0.0	13.245137	1
AAGACGG	625	0.0	13.071429	5
CAAGACG	625	0.0	13.069963	4
CGCATCG	560	0.0	12.8948965	13
GTTCTAA	120	7.512426E-10	12.656465	1
CGCCAGT	580	0.0	12.451363	18
ATCGCCA	595	0.0	12.136917	16
TTAGTGC	55	0.003077395	12.086586	3
GTTATTC	260	0.0	12.053383	3
CGCAAGA	630	0.0	11.905771	2
CGAACGA	225	0.0	11.824623	16
TCGCGTA	290	0.0	11.794175	9
AATCGTA	65	8.015177E-4	11.694158	13
CATCGCC	635	0.0	11.671137	15
CGGTCCA	570	0.0	11.6677265	10
CGACCAT	355	0.0	11.508088	10
CTTATGC	75	2.081486E-4	11.395925	3
GTATAGG	125	1.8550054E-8	11.390819	1
>>END_MODULE