FastQCFastQC Report
Thu 2 Feb 2017
SRR4062489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233251
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT33200.14866219694964874No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC32520.14561730857839086No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29890.1338407550248494No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29050.13007942233094266No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA27710.12407920112875803No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA27530.12327320126577801No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25870.11584009141829557No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT25160.11266086973654103No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24730.11073542561942208No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24660.11042198122826319No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23760.10639198191336308No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22730.10177987158631072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC1851.8189894E-1219.0268424
TAGGACG2000.018.699824
CGCAATA10400.017.1344536
ACGGTAT9700.017.0101459
ATCGTTT8200.016.90227529
CGACGGT9700.016.7833427
ATACGAA10500.016.76174440
GACGGTA9850.016.7511088
CTACGAC9750.016.6972734
TACGACG9800.016.6120855
CGAATGC10350.016.5795543
CGTTTAT8400.016.4998431
AGAGCGA11550.016.38079515
ACGACGG9950.016.361656
CGTCTTA3500.016.34270115
TACGAAT10950.016.27381541
ATACCGT7850.016.254626
AAGACGG12050.016.2488045
GTATTAA3400.016.1813871
GAGCGAA11900.016.08387816
Produced by FastQC (version 0.11.5)