##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062489_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2233251 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32092093544344 32.0 32.0 32.0 32.0 32.0 2 31.457735382184985 32.0 32.0 32.0 32.0 32.0 3 31.523988123144242 32.0 32.0 32.0 32.0 32.0 4 31.612348992567338 32.0 32.0 32.0 32.0 32.0 5 31.55374384697466 32.0 32.0 32.0 32.0 32.0 6 35.1541658326807 36.0 36.0 36.0 36.0 36.0 7 35.15965066174827 36.0 36.0 36.0 36.0 36.0 8 35.111453213275176 36.0 36.0 36.0 36.0 36.0 9 35.24229699214285 36.0 36.0 36.0 36.0 36.0 10 35.10952015693713 36.0 36.0 36.0 36.0 36.0 11 35.23767682181716 36.0 36.0 36.0 36.0 36.0 12 35.14716796275923 36.0 36.0 36.0 36.0 36.0 13 35.19072464313236 36.0 36.0 36.0 36.0 36.0 14 35.15185350862935 36.0 36.0 36.0 36.0 36.0 15 35.11634563244346 36.0 36.0 36.0 36.0 36.0 16 35.12421353443926 36.0 36.0 36.0 36.0 36.0 17 35.09311582083698 36.0 36.0 36.0 36.0 36.0 18 35.10723738621409 36.0 36.0 36.0 36.0 36.0 19 35.090408109075064 36.0 36.0 36.0 36.0 36.0 20 35.09050617239173 36.0 36.0 36.0 36.0 36.0 21 35.080425352994354 36.0 36.0 36.0 36.0 36.0 22 35.066901570848955 36.0 36.0 36.0 36.0 36.0 23 35.02624559442714 36.0 36.0 36.0 36.0 36.0 24 35.00368700159543 36.0 36.0 36.0 36.0 36.0 25 34.973515292280176 36.0 36.0 36.0 36.0 36.0 26 34.93563240316471 36.0 36.0 36.0 32.0 36.0 27 34.923924359599525 36.0 36.0 36.0 32.0 36.0 28 34.90254297434547 36.0 36.0 36.0 32.0 36.0 29 34.87931495384979 36.0 36.0 36.0 32.0 36.0 30 34.87247917945632 36.0 36.0 36.0 32.0 36.0 31 34.8707713553022 36.0 36.0 36.0 32.0 36.0 32 34.83517437135369 36.0 36.0 36.0 32.0 36.0 33 34.814617344848386 36.0 36.0 36.0 32.0 36.0 34 34.80307766569902 36.0 36.0 36.0 32.0 36.0 35 34.770103315749104 36.0 36.0 36.0 32.0 36.0 36 34.749675696999574 36.0 36.0 36.0 32.0 36.0 37 34.73522591056715 36.0 36.0 36.0 32.0 36.0 38 34.702752176087685 36.0 36.0 36.0 32.0 36.0 39 34.68802566303564 36.0 36.0 36.0 32.0 36.0 40 34.67177804913106 36.0 36.0 36.0 32.0 36.0 41 34.64891944523925 36.0 36.0 36.0 32.0 36.0 42 34.60867587208066 36.0 36.0 36.0 32.0 36.0 43 34.61064788507875 36.0 36.0 36.0 32.0 36.0 44 34.57465909564129 36.0 36.0 36.0 32.0 36.0 45 34.52197804904151 36.0 36.0 36.0 32.0 36.0 46 34.54919845552515 36.0 36.0 36.0 32.0 36.0 47 34.50691973271253 36.0 36.0 36.0 32.0 36.0 48 34.46541253087987 36.0 36.0 36.0 32.0 36.0 49 34.46659690290075 36.0 36.0 36.0 32.0 36.0 50 33.95591897193822 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 10.0 21 32.0 22 159.0 23 462.0 24 1327.0 25 3091.0 26 6615.0 27 12458.0 28 21336.0 29 33234.0 30 48847.0 31 69795.0 32 103469.0 33 169469.0 34 378454.0 35 1384491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66964202142596 18.2214242560904 11.794666932987116 26.314266789496525 2 16.59172784692509 20.339430088820524 35.81032216751636 27.258519896738022 3 18.56223180770173 23.42794479229496 28.701630233038202 29.30819316696511 4 12.683667301018694 15.426038285010634 35.24074778909661 36.64954662487406 5 14.855517807895305 36.11671952682435 33.003276389443016 16.024486275837333 6 35.27380161836541 34.76028785070666 16.219073534831217 13.746836996096704 7 30.77511732905735 30.293934979932825 20.43883149617441 18.49211619483542 8 28.722387228305283 32.096839987981646 19.374266484152475 19.806506299560596 9 27.310320246134445 14.157432371014275 18.79072258335494 39.74152479949634 10 16.4588754242134 26.201488323524764 30.633009903499424 26.70662634876241 11 38.01829709244505 20.921875776614453 21.726487528719343 19.333339602221155 12 24.883588992012093 23.570615215217636 27.949925915179264 23.59586987759101 13 29.35944056445066 18.778229585478748 25.418593789950165 26.443736060120425 14 23.826609727254127 19.618663553716083 24.398108407877125 32.15661831115266 15 25.487820222626116 26.85909465617613 22.000393148822052 25.652691972375695 16 26.540456043678027 25.44201256374675 23.224617385148377 24.79291400742684 17 24.531680496280984 25.75783017672443 24.770972900045717 24.939516426948874 18 25.686499188850693 24.20386244089894 25.945672922568935 24.16396544768143 19 25.839639162816898 25.011116081443603 24.968039866544334 24.18120488919517 20 25.91289559480775 23.919837044738816 24.77406256618714 25.393204794266293 21 27.225690260521546 24.041587801818963 23.97285392461483 24.75986801304466 22 25.967658807720223 24.148696228054973 24.60610115029614 25.27754381392866 23 24.409437183729015 23.71820274568331 25.36866657621557 26.503693494372104 24 24.952188535905726 24.986622641163038 24.783600231232406 25.27758859169883 25 25.27678259183585 24.111709789898224 24.83464688922114 25.776860729044788 26 24.85833432963872 25.035116966252335 25.378652018962487 24.72789668514645 27 25.913343372509406 24.292611981367074 24.49742550210433 25.29661914401919 28 24.735598461614927 24.205026662923245 25.557494433003725 25.501880442458102 29 24.679536693367652 24.658267252539012 25.4697747812494 25.19242127284394 30 24.786958563994823 24.7366731280989 25.644542418205567 24.83182588970071 31 25.297559477192667 24.611967038187828 24.293328425689722 25.797145058929782 32 25.062655294904157 24.413243294193084 24.413780627435074 26.11032078346769 33 24.587652708987928 24.017989916941715 25.17083838762414 26.223518986446216 34 25.48535744526701 24.24286387871314 25.29290258909545 24.978876086924398 35 26.20453321189602 24.093350904130347 25.120194729566897 24.58192115440674 36 24.77871945428436 24.864424106381236 24.748404903882275 25.608451535452126 37 25.99806291366264 24.49370894718059 24.641206922105933 24.867021217050837 38 24.88596221383087 24.098231681078392 25.304052253866672 25.711753851224067 39 25.47541678029026 24.074812907281807 24.613220815752463 25.836549496675477 40 25.70790296298983 24.307299089981377 25.271767481577307 24.71303046545149 41 24.39443663072355 24.707119799789634 25.766293175285714 25.132150394201098 42 26.161725663617748 24.71029902147139 24.97775664267026 24.1502186722406 43 25.088089068358194 23.501344004771518 25.286633701272272 26.123933225598016 44 24.670267694943384 24.232161991643572 25.1994961605301 25.89807415288295 45 24.611295371635343 24.58362270967303 25.287305367824754 25.517776550866877 46 24.46169187734272 24.164053505948964 25.62331887158026 25.750935745128057 47 24.6734916943953 23.992377032407017 25.99282391455327 25.341307358644414 48 25.591659871640044 25.050968296890947 24.579324043737135 24.77804778773188 49 24.203280329886788 24.893843101380007 25.23894537604595 25.66393119268725 50 24.19509683197134 25.232105675584908 24.993529609313782 25.57926788312997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 28.0 2 35.0 3 99.0 4 163.0 5 205.5 6 248.0 7 263.0 8 278.0 9 368.5 10 459.0 11 691.5 12 924.0 13 1734.0 14 2544.0 15 3404.0 16 4264.0 17 5036.5 18 5809.0 19 6282.0 20 6755.0 21 7738.0 22 8721.0 23 9484.0 24 10247.0 25 11890.0 26 13533.0 27 16798.5 28 20064.0 29 22801.0 30 25538.0 31 27777.5 32 30017.0 33 34842.5 34 39668.0 35 46258.5 36 52849.0 37 65463.5 38 78078.0 39 85603.0 40 93128.0 41 103079.5 42 113031.0 43 115555.5 44 118080.0 45 131972.0 46 145864.0 47 158257.5 48 170651.0 49 174561.5 50 178472.0 51 168262.5 52 158053.0 53 155594.5 54 153136.0 55 156786.5 56 160437.0 57 157064.5 58 153692.0 59 141719.0 60 129746.0 61 113992.0 62 98238.0 63 85684.5 64 73131.0 65 62747.5 66 52364.0 67 46538.0 68 40712.0 69 37849.0 70 34986.0 71 27174.0 72 19362.0 73 17653.5 74 15945.0 75 11556.0 76 7167.0 77 6567.0 78 5967.0 79 5201.0 80 4435.0 81 3515.0 82 2595.0 83 2235.0 84 1875.0 85 1523.5 86 1172.0 87 876.0 88 580.0 89 359.0 90 138.0 91 89.0 92 40.0 93 29.0 94 18.0 95 13.5 96 9.0 97 7.0 98 5.0 99 6.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01576177509827601 2 0.0042986659358934575 3 5.373332419866822E-4 4 4.4777770165556846E-5 5 0.0 6 4.477777016555685E-4 7 8.955554033111369E-5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.7911108066222739E-4 47 0.0 48 0.0 49 0.0 50 4.4777770165556846E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2233251.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.48847398540957 #Duplication Level Percentage of deduplicated Percentage of total 1 80.02612381778268 41.204229943450684 2 12.190296430192113 12.553195212407555 3 3.333898406001521 5.149720240422233 4 1.3772119611990468 2.8364216894636805 5 0.7615164567516332 1.9604660136458871 6 0.47722658325602 1.4743001110274088 7 0.32557579628701 1.1734380642181903 8 0.2312317192941307 0.9524614690781901 9 0.17568258267319617 0.8141065278892584 >10 0.9185111108951063 8.565646820645444 >50 0.07544364543481186 2.753435588012149 >100 0.08532146412287096 9.501384181157531 >500 0.014523363881390862 5.070618680927617 >1k 0.007436662228423026 5.990575457654251 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 3320 0.14866219694964874 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 3252 0.14561730857839086 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 2989 0.1338407550248494 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 2905 0.13007942233094266 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 2771 0.12407920112875803 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 2753 0.12327320126577801 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC 2587 0.11584009141829557 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT 2516 0.11266086973654103 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG 2473 0.11073542561942208 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 2466 0.11042198122826319 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA 2376 0.10639198191336308 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 2273 0.10177987158631072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3433331049667055E-4 2 0.0 0.0 0.0 0.0 1.3433331049667055E-4 3 0.0 0.0 0.0 0.0 1.3433331049667055E-4 4 0.0 0.0 0.0 0.0 1.3433331049667055E-4 5 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 6 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 7 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 8 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 9 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 10 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 11 0.0 0.0 0.0 4.4777770165556846E-5 1.7911108066222739E-4 12 0.0 0.0 0.0 4.4777770165556846E-5 3.5822216132445477E-4 13 0.0 0.0 0.0 4.4777770165556846E-5 3.5822216132445477E-4 14 0.0 0.0 0.0 4.4777770165556846E-5 3.5822216132445477E-4 15 0.0 0.0 0.0 4.4777770165556846E-5 4.925554718211253E-4 16 0.0 0.0 0.0 4.4777770165556846E-5 4.925554718211253E-4 17 0.0 0.0 0.0 4.4777770165556846E-5 5.373332419866822E-4 18 0.0 0.0 0.0 4.4777770165556846E-5 7.612220928144665E-4 19 0.0 0.0 0.0 8.955554033111369E-5 7.612220928144665E-4 20 0.0 0.0 0.0 2.2388885082778425E-4 7.612220928144665E-4 21 0.0 0.0 0.0 4.0299993149001167E-4 8.507776331455802E-4 22 0.0 0.0 0.0 7.612220928144665E-4 9.403331734766938E-4 23 0.0 0.0 0.0 0.0013433331049667054 0.0010298887138078076 24 0.0 0.0 0.0 0.0022388885082778426 0.0010746664839733644 25 0.0 0.0 0.0 0.0027762217502645247 0.0010746664839733644 26 0.0 0.0 0.0 0.004029999314900117 0.001164222024304478 27 0.0 0.0 0.0 0.005552443500529049 0.0013433331049667054 28 0.0 0.0 0.0 0.012851220037514816 0.0013433331049667054 29 0.0 0.0 0.0 0.026687551018671884 0.0013433331049667054 30 0.0 0.0 0.0 0.05633043486827052 0.0013433331049667054 31 0.0 0.0 0.0 0.12246720140279799 0.0013433331049667054 32 0.0 0.0 0.0 0.19845507737374796 0.0013433331049667054 33 0.0 0.0 0.0 0.28084617447837257 0.0013433331049667054 34 0.0 0.0 0.0 0.37899904668127316 0.001432888645297819 35 0.0 0.0 0.0 0.4920181385791387 0.001432888645297819 36 0.0 0.0 0.0 0.6489642230094155 0.001432888645297819 37 0.0 0.0 0.0 0.8663155193930284 0.001432888645297819 38 0.0 0.0 0.0 1.1447884720526265 0.0015672219557944897 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACC 185 1.8189894E-12 19.026842 4 TAGGACG 200 0.0 18.69982 4 CGCAATA 1040 0.0 17.13445 36 ACGGTAT 970 0.0 17.010145 9 ATCGTTT 820 0.0 16.902275 29 CGACGGT 970 0.0 16.783342 7 ATACGAA 1050 0.0 16.761744 40 GACGGTA 985 0.0 16.751108 8 CTACGAC 975 0.0 16.697273 4 TACGACG 980 0.0 16.612085 5 CGAATGC 1035 0.0 16.57955 43 CGTTTAT 840 0.0 16.49984 31 AGAGCGA 1155 0.0 16.380795 15 ACGACGG 995 0.0 16.36165 6 CGTCTTA 350 0.0 16.342701 15 TACGAAT 1095 0.0 16.273815 41 ATACCGT 785 0.0 16.25462 6 AAGACGG 1205 0.0 16.248804 5 GTATTAA 340 0.0 16.181387 1 GAGCGAA 1190 0.0 16.083878 16 >>END_MODULE