FastQCFastQC Report
Thu 2 Feb 2017
SRR4062488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288925
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG48150.3735671198867273No Hit
TCCATGTACTCTGCGTTGATACCAC38180.296215838780379No Hit
GAGTACATGGAAGCAGTGGTATCAA21170.16424539829703047No Hit
GTATCAACGCAGAGTACTTTTTTTT19470.1510561126520162No Hit
CATGTACTCTGCGTTGATACCACTG17870.1386426673390616No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13950.10822972632232286No Hit
TATCAACGCAGAGTACTTTTTTTTT13760.1067556296914095No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13580.10535911709370213No Hit
GAATAGGACCGCGGTTCTATTTTGT13320.103341932230347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTT801.2927921E-714.2455154
TCTATAC801.2940109E-714.244413
GAGGTTA903.6443453E-813.7200355
CGTTATT1051.382432E-913.563472
CGCGTAA1001.00499165E-813.30149610
GCGTTAT1001.0175427E-813.289621
GTCTAAT655.4911434E-513.1435821
GCGTAAC1051.989065E-812.66809111
AACCGCG1207.403287E-1012.66661557
CGACCCG1800.012.6646475
AATCGAA1155.320544E-912.3912547
TCGGTTG550.003060913212.09461917
CGGTCCA2200.012.09226910
CGGACGG550.003071619212.0889825
TCCCGAC1900.011.9929632
TCCAACG1353.6925485E-1011.96709718
GTTATTC1353.7289283E-1011.9582693
TACGCTA2150.011.931119
ATATACG2150.011.929726
GTCTTAT1052.7459464E-711.7527271
Produced by FastQC (version 0.11.5)