##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062488_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288925
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159274589289524	32.0	32.0	32.0	32.0	32.0
2	30.691860271156195	32.0	32.0	32.0	32.0	32.0
3	30.691742343425723	32.0	32.0	32.0	32.0	32.0
4	30.783788040421282	32.0	32.0	32.0	32.0	32.0
5	30.487003510677503	32.0	32.0	32.0	32.0	32.0
6	34.19248443470334	36.0	36.0	36.0	32.0	36.0
7	34.079477859456524	36.0	36.0	36.0	32.0	36.0
8	34.05084702368253	36.0	36.0	36.0	32.0	36.0
9	34.28392730376088	36.0	36.0	36.0	32.0	36.0
10	33.819114378260956	36.0	36.0	36.0	32.0	36.0
11	34.26994666097717	36.0	36.0	36.0	32.0	36.0
12	33.97314273522509	36.0	36.0	36.0	32.0	36.0
13	34.13328005896386	36.0	36.0	36.0	32.0	36.0
14	34.00390402855093	36.0	36.0	36.0	32.0	36.0
15	33.92301181216906	36.0	36.0	36.0	32.0	36.0
16	33.92149194095855	36.0	36.0	36.0	32.0	36.0
17	33.83739240064395	36.0	36.0	36.0	32.0	36.0
18	33.860925965436316	36.0	36.0	36.0	32.0	36.0
19	33.845773803751186	36.0	36.0	36.0	32.0	36.0
20	33.84349593653626	36.0	36.0	36.0	32.0	36.0
21	33.81069573481778	36.0	36.0	36.0	32.0	36.0
22	33.758925461140095	36.0	36.0	36.0	32.0	36.0
23	33.691584847838314	36.0	36.0	36.0	27.0	36.0
24	33.659198944857145	36.0	36.0	36.0	27.0	36.0
25	33.16332059662122	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	41.0
5	132.0
6	220.0
7	50.0
8	178.0
9	232.0
10	161.0
11	51.0
12	94.0
13	74.0
14	212.0
15	260.0
16	416.0
17	566.0
18	776.0
19	1144.0
20	1875.0
21	2775.0
22	4218.0
23	6252.0
24	8942.0
25	12735.0
26	17644.0
27	22266.0
28	30204.0
29	39974.0
30	50863.0
31	69191.0
32	97639.0
33	136960.0
34	289997.0
35	492775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95816192410768	16.45249242333721	10.750015905948514	25.839329746606605
2	16.77917958800176	19.293282819544586	37.15098817663895	26.776549415814703
3	19.847340681815183	23.033510411728606	27.92262757752255	29.196521328933656
4	12.586654003193804	14.643769990362724	35.40232132631255	37.36725468013092
5	14.515317882737522	36.49376714210543	33.706482141166354	15.28443283399069
6	35.412138918556415	34.49391160179827	16.489670524825513	13.6042789548198
7	30.79545904468523	29.94249665592474	20.191149903850558	19.070894395539472
8	28.50394935875335	33.30599998136362	19.087143695779794	19.102906964103234
9	27.078992593789096	14.122122880819568	18.103087435534437	40.6957970898569
10	15.574566980448386	26.938441665572743	31.86491048327149	25.622080870707382
11	38.18855771747802	20.675043868064126	21.561338001200888	19.575060413256974
12	24.426256889928176	23.31672111279866	28.91489700552748	23.34212499174568
13	30.226968898293173	19.08778994482494	24.990270764053793	25.694970392828097
14	23.462123148220794	19.423028672234206	24.699089520042445	32.41575865950256
15	25.419914980080982	27.275191597739116	22.260134854360864	25.044758567819038
16	26.119078965253422	25.436264286796145	24.04857607779629	24.39608067015414
17	24.118894052889274	25.903050575182295	25.405431858628436	24.572623513299998
18	25.231871166716513	24.55468850141193	26.521757707351746	23.691682624519807
19	25.54120591850021	25.452875892040044	24.70179876291351	24.30411942654624
20	25.3859084384069	24.366995196341016	25.523555329078608	24.72354103617348
21	26.206146483950153	24.54926820173394	23.90913893291072	25.335446381405173
22	25.27497119164414	24.86640926280913	24.69826015402272	25.16035939152401
23	24.14476807289526	24.518707457888116	25.157115919655475	26.179408549561145
24	24.53411251220704	25.427469784418026	24.965447373961577	25.07297032941336
25	25.119693981158154	24.566883745934135	24.81912866355251	25.4942936093552
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	130.0
2	326.0
3	522.0
4	522.0
5	522.0
6	1284.0
7	2046.0
8	2046.0
9	2046.0
10	2222.5
11	2399.0
12	2399.0
13	2399.0
14	2780.0
15	3161.0
16	3161.0
17	3161.0
18	5110.0
19	7059.0
20	7059.0
21	7059.0
22	11502.5
23	15946.0
24	15946.0
25	15946.0
26	24147.0
27	32348.0
28	32348.0
29	32348.0
30	42994.0
31	53640.0
32	53640.0
33	53640.0
34	65308.0
35	76976.0
36	76976.0
37	76976.0
38	88537.0
39	100098.0
40	100098.0
41	100098.0
42	116991.0
43	133884.0
44	133884.0
45	133884.0
46	157277.0
47	180670.0
48	180670.0
49	180670.0
50	178120.0
51	175570.0
52	175570.0
53	175570.0
54	162842.5
55	150115.0
56	150115.0
57	150115.0
58	138221.0
59	126327.0
60	126327.0
61	126327.0
62	111437.5
63	96548.0
64	96548.0
65	96548.0
66	79855.5
67	63163.0
68	63163.0
69	63163.0
70	47680.0
71	32197.0
72	32197.0
73	32197.0
74	24972.5
75	17748.0
76	17748.0
77	17748.0
78	14248.0
79	10748.0
80	10748.0
81	10748.0
82	7507.5
83	4267.0
84	4267.0
85	4267.0
86	3106.5
87	1946.0
88	1946.0
89	1946.0
90	1326.5
91	707.0
92	707.0
93	707.0
94	431.5
95	156.0
96	156.0
97	156.0
98	355.0
99	554.0
100	554.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007680819287390654
2	0.002793025195414784
3	0.003258529394650581
4	0.01388754194386795
5	0.033438718311771434
6	0.044455651027018636
7	0.0640844114281281
8	0.08697170122388813
9	0.0948076885776907
10	0.11730705820742092
11	0.1196345792035999
12	0.13344453711426188
13	0.12165176406695502
14	0.12273794053183854
15	0.11342785654712262
16	0.11327268848071066
17	0.12366894893031015
18	0.13034117578602325
19	0.13243594468258432
20	0.12203968423298486
21	0.13072909595205306
22	0.15299571348216537
23	0.14104777236844657
24	0.1363927303760886
25	0.1306515119188471
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1288925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.473394536244335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35769739727165	43.228831598094686
2	12.412676347492834	13.523212318553707
3	3.5664489395970915	5.828297405401278
4	1.5125765262607223	3.2958071152504904
5	0.7860605231213498	2.140969250267795
6	0.4800690134990278	1.5690593266174886
7	0.3332400518676386	1.2706901774465083
8	0.23001578935061776	1.0023792674289493
9	0.1721413353463735	0.843941058868705
>10	0.9792283202878792	9.684785270222696
>50	0.0813394295494937	3.0580934485253874
>100	0.07712288844019094	8.879681485950957
>500	0.008537578436391443	3.1834101636725802
>1k	0.002845859478797148	2.490842113699011
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4815	0.3735671198867273	No Hit
TCCATGTACTCTGCGTTGATACCAC	3818	0.296215838780379	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2117	0.16424539829703047	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1947	0.1510561126520162	No Hit
CATGTACTCTGCGTTGATACCACTG	1787	0.1386426673390616	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1395	0.10822972632232286	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1376	0.1067556296914095	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1358	0.10535911709370213	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1332	0.103341932230347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.758403320596621E-5
2	0.0	0.0	0.0	0.0	7.758403320596621E-5
3	0.0	0.0	0.0	0.0	7.758403320596621E-5
4	0.0	0.0	0.0	0.0	7.758403320596621E-5
5	0.0	0.0	0.0	0.0	7.758403320596621E-5
6	0.0	0.0	0.0	0.0	1.5516806641193242E-4
7	0.0	0.0	0.0	0.0	1.5516806641193242E-4
8	0.0	0.0	0.0	0.0	1.5516806641193242E-4
9	0.0	0.0	0.0	0.0	1.5516806641193242E-4
10	0.0	0.0	0.0	0.0	1.5516806641193242E-4
11	0.0	0.0	0.0	0.0	1.5516806641193242E-4
12	0.0	0.0	0.0	0.0	3.8792016602983104E-4
13	0.0	0.0	0.0	0.0	4.655041992357973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGGTT	80	1.2927921E-7	14.245515	4
TCTATAC	80	1.2940109E-7	14.24441	3
GAGGTTA	90	3.6443453E-8	13.720035	5
CGTTATT	105	1.382432E-9	13.56347	2
CGCGTAA	100	1.00499165E-8	13.301496	10
GCGTTAT	100	1.0175427E-8	13.28962	1
GTCTAAT	65	5.4911434E-5	13.143582	1
GCGTAAC	105	1.989065E-8	12.668091	11
AACCGCG	120	7.403287E-10	12.6666155	7
CGACCCG	180	0.0	12.664647	5
AATCGAA	115	5.320544E-9	12.391254	7
TCGGTTG	55	0.0030609132	12.094619	17
CGGTCCA	220	0.0	12.092269	10
CGGACGG	55	0.0030716192	12.088982	5
TCCCGAC	190	0.0	11.992963	2
TCCAACG	135	3.6925485E-10	11.967097	18
GTTATTC	135	3.7289283E-10	11.958269	3
TACGCTA	215	0.0	11.93111	9
ATATACG	215	0.0	11.92972	6
GTCTTAT	105	2.7459464E-7	11.752727	1
>>END_MODULE