FastQCFastQC Report
Thu 2 Feb 2017
SRR4062488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288925
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15660.12149659600054309No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14680.11389336074635839No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13980.10846247842194078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3150.021.6589491
CGCAATA3300.021.3330936
CAATACG3250.020.30745938
TAGCGGC3300.019.99977130
CTAGCGG3300.019.99977129
GTCTTAA1452.9467628E-1019.7315651
CCCTATA1252.2864697E-819.361282
TAGGACG4100.019.316854
GTAGGAC6700.019.0452983
TGTAGGA7250.018.8150372
TCTAGCG3550.018.59133728
GATTTCG2250.018.57756641
GCGCAAT3700.018.4322235
AAGACGG4550.018.3734175
TACGAAT3600.018.33312241
CGAATGC3600.018.33312243
GACGTGA2900.018.2066867
ATACGAA3650.018.08198440
ATACACT4550.017.8899064
AATACGA3700.017.83763339
Produced by FastQC (version 0.11.5)