##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288925
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245655100180382	32.0	32.0	32.0	32.0	32.0
2	31.387593537250034	32.0	32.0	32.0	32.0	32.0
3	31.503123145256705	32.0	32.0	32.0	32.0	32.0
4	31.616041274705665	32.0	32.0	32.0	32.0	32.0
5	31.545672556587853	32.0	32.0	32.0	32.0	32.0
6	35.15299261012084	36.0	36.0	36.0	36.0	36.0
7	35.17235292976705	36.0	36.0	36.0	36.0	36.0
8	35.10852221812751	36.0	36.0	36.0	36.0	36.0
9	35.24506701320868	36.0	36.0	36.0	36.0	36.0
10	35.11558546851058	36.0	36.0	36.0	36.0	36.0
11	35.25160579552728	36.0	36.0	36.0	36.0	36.0
12	35.159113990340785	36.0	36.0	36.0	36.0	36.0
13	35.20075722016409	36.0	36.0	36.0	36.0	36.0
14	35.16230890082821	36.0	36.0	36.0	36.0	36.0
15	35.12199235797273	36.0	36.0	36.0	36.0	36.0
16	35.124222123087065	36.0	36.0	36.0	36.0	36.0
17	35.111126714122236	36.0	36.0	36.0	36.0	36.0
18	35.115455903175125	36.0	36.0	36.0	36.0	36.0
19	35.104696549450125	36.0	36.0	36.0	36.0	36.0
20	35.100233915860116	36.0	36.0	36.0	36.0	36.0
21	35.08332602750354	36.0	36.0	36.0	36.0	36.0
22	35.0619904183719	36.0	36.0	36.0	36.0	36.0
23	35.010553756037005	36.0	36.0	36.0	36.0	36.0
24	34.98691855616114	36.0	36.0	36.0	36.0	36.0
25	34.96062532730764	36.0	36.0	36.0	32.0	36.0
26	34.905660918982875	36.0	36.0	36.0	32.0	36.0
27	34.881747192427795	36.0	36.0	36.0	32.0	36.0
28	34.86150629400469	36.0	36.0	36.0	32.0	36.0
29	34.83118955718913	36.0	36.0	36.0	32.0	36.0
30	34.81678452974378	36.0	36.0	36.0	32.0	36.0
31	34.81538025874275	36.0	36.0	36.0	32.0	36.0
32	34.77118218670598	36.0	36.0	36.0	32.0	36.0
33	34.746824679480966	36.0	36.0	36.0	32.0	36.0
34	34.73898093372384	36.0	36.0	36.0	32.0	36.0
35	34.69443140601664	36.0	36.0	36.0	32.0	36.0
36	34.66477878852532	36.0	36.0	36.0	32.0	36.0
37	34.635684776073084	36.0	36.0	36.0	32.0	36.0
38	34.5892988342999	36.0	36.0	36.0	32.0	36.0
39	34.545264464573194	36.0	36.0	36.0	32.0	36.0
40	34.512047636596385	36.0	36.0	36.0	32.0	36.0
41	34.50132785072832	36.0	36.0	36.0	32.0	36.0
42	34.4309009445856	36.0	36.0	36.0	32.0	36.0
43	34.439597338867664	36.0	36.0	36.0	32.0	36.0
44	34.421852318792794	36.0	36.0	36.0	32.0	36.0
45	34.33307601295653	36.0	36.0	36.0	32.0	36.0
46	34.37999728455884	36.0	36.0	36.0	32.0	36.0
47	34.32833174932599	36.0	36.0	36.0	32.0	36.0
48	34.302399286226894	36.0	36.0	36.0	32.0	36.0
49	34.311727214539246	36.0	36.0	36.0	32.0	36.0
50	33.79225478596505	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	29.0
22	91.0
23	314.0
24	904.0
25	2124.0
26	4569.0
27	8531.0
28	14085.0
29	20884.0
30	29717.0
31	42323.0
32	62899.0
33	98914.0
34	219574.0
35	783954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28243030902636	17.104347320025607	11.292711788783489	26.32051058216454
2	16.240700208631882	19.788140491731923	36.74442710802383	27.226732191612363
3	19.311606515252375	23.21272016596918	28.27163022513527	29.20404309364318
4	12.365148833791986	15.216215733935595	35.51288248077268	36.90575295149974
5	14.295633958531335	36.60406928254165	33.62709234439553	15.473204414531489
6	35.533026823966075	34.82359677311738	16.16443818943272	13.478938213483826
7	30.77062728291195	29.83923024165893	20.248051863414755	19.14209061201436
8	28.46185774967512	33.366254824757064	19.061155614174602	19.110731811393215
9	27.118024710514575	13.859534107880597	17.97777217448649	41.04466900711834
10	15.410361347634657	26.949434606358015	31.925674496188684	25.71452954981865
11	38.433190449405515	20.474193610954867	21.568438815291813	19.52417712434781
12	24.250674011288474	23.251857167794867	29.041565645790097	23.455903175126558
13	30.421785596524238	18.865100762263125	24.968403902476872	25.744709738735764
14	23.37428477219388	19.247046957736096	24.801598231084043	32.57707003898598
15	25.53267257598386	27.260391411447525	22.081036522683632	25.125899489884983
16	26.042710010279883	25.46734682002444	24.0650154198266	24.424927749869077
17	24.218786973640825	25.81950074674632	25.326066295556377	24.63564598405648
18	25.31396318637624	24.38062726690847	26.568497003316715	23.73691254339857
19	25.644781503966485	25.28960179994957	24.760401109451674	24.30521558663227
20	25.414124173245145	24.175262331012277	25.646255600597396	24.764357895145178
21	26.213705219465833	24.393195880287838	24.101402331400198	25.291696568846135
22	25.361599782764703	24.675601761157555	24.856139806427837	25.106658649649905
23	24.27775083887736	24.22794188955913	25.223112283492057	26.271194988071457
24	24.655973000756447	25.20790581298369	25.02822119207867	25.1078999941812
25	25.32404911069302	24.274026805283473	24.97453304110014	25.427391042923364
26	24.298931279942586	25.105650057218227	25.56580095816281	25.029617704676376
27	25.366177240723857	24.761875206082586	25.115115309269353	24.7568322439242
28	24.623310122776733	24.433151657388912	25.323583606493784	25.619954613340575
29	24.38031693077565	24.768702601004712	25.51715576934267	25.333824698876974
30	24.795779428593594	24.800589638652365	25.695521461683185	24.708109471070856
31	25.08493512035223	24.553639660957774	24.669938126733516	25.69148709195648
32	24.63603390422251	24.991756696471867	24.87863917605757	25.493570223248057
33	24.26696665826173	24.4044455651027	25.536163857478133	25.792423919157436
34	24.864906802180112	24.95280951180247	25.763252322671992	24.41903136334542
35	25.671315243322923	24.58583703473825	25.470682933452295	24.27216478848653
36	24.41181604825727	25.252749384176738	25.212172934810017	25.123261632755977
37	25.504121651764066	24.723238357546016	25.063677095253794	24.70896289543612
38	24.821149407451944	24.690730647632716	25.026204007215313	25.46191593770002
39	25.285645014256065	24.32717186802956	24.98617064608104	25.40101247163334
40	25.731287700991135	24.665748588940396	25.058401380995793	24.54456232907268
41	24.399712939077137	24.710747328199854	25.956514149388056	24.93302558333495
42	25.97187578796284	25.275248753806466	25.094788292569387	23.658087165661307
43	24.8920612138022	24.030102604883915	25.38270263979673	25.69513354151716
44	24.444634094303392	24.846984890509532	25.489613437554553	25.218767577632523
45	24.66799852590337	24.982369028453945	25.246620245553466	25.10301220008922
46	24.435070547325594	24.36446891278138	25.70186559000467	25.49859494988836
47	25.014178482068388	24.34253350660434	25.84603448610276	24.79725352522451
48	25.162596737591404	25.577050642977678	24.45177182535834	24.80858079407258
49	24.426712182632816	24.92767228504374	25.545629109529262	25.099986422794192
50	24.30769827569486	25.836491650018427	24.456659619450317	25.399150454836395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	12.5
2	16.0
3	83.0
4	150.0
5	165.5
6	181.0
7	176.5
8	172.0
9	224.0
10	276.0
11	411.0
12	546.0
13	961.5
14	1377.0
15	1897.0
16	2417.0
17	2631.5
18	2846.0
19	3080.5
20	3315.0
21	3770.5
22	4226.0
23	4753.5
24	5281.0
25	6153.5
26	7026.0
27	9551.5
28	12077.0
29	14107.0
30	16137.0
31	17931.5
32	19726.0
33	23241.5
34	26757.0
35	31179.0
36	35601.0
37	41361.0
38	47121.0
39	50541.0
40	53961.0
41	58438.5
42	62916.0
43	66447.5
44	69979.0
45	78187.5
46	86396.0
47	93370.0
48	100344.0
49	101417.5
50	102491.0
51	98399.0
52	94307.0
53	92360.0
54	90413.0
55	91223.0
56	92033.0
57	89051.0
58	86069.0
59	78938.0
60	71807.0
61	63462.5
62	55118.0
63	48172.0
64	41226.0
65	35380.5
66	29535.0
67	25366.5
68	21198.0
69	19477.0
70	17756.0
71	13717.5
72	9679.0
73	8670.0
74	7661.0
75	5586.0
76	3511.0
77	3062.0
78	2613.0
79	2271.5
80	1930.0
81	1518.5
82	1107.0
83	945.5
84	784.0
85	648.0
86	512.0
87	365.0
88	218.0
89	136.5
90	55.0
91	40.5
92	26.0
93	17.5
94	9.0
95	6.0
96	3.0
97	5.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015516806641193242
2	0.0040343697267102435
3	8.534243652656284E-4
4	3.103361328238649E-4
5	0.0
6	2.3275209961789862E-4
7	7.758403320596622E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.3275209961789862E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1288925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.740565752607395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.51776389448536	45.91400674649842
2	12.562952735350692	14.507839969248309
3	3.5229676175072964	6.102544300889599
4	1.4210507419999605	3.2820909522496096
5	0.7591073876436407	2.1915645014763836
6	0.47268536956391544	1.6375872396960471
7	0.3177075450330433	1.284122937585594
8	0.2253286168050899	1.0408481451662694
9	0.16855914093316804	0.8759430144229144
>10	0.8893186349157679	9.237756193512087
>50	0.07052308796654701	2.8485301101317066
>100	0.06436046102559988	7.686870533524928
>500	0.006462961490524199	2.4989511927855315
>1k	0.0012118052794732874	0.8913441628126224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1566	0.12149659600054309	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1468	0.11389336074635839	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1398	0.10846247842194078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5516806641193242E-4
2	0.0	0.0	0.0	0.0	1.5516806641193242E-4
3	0.0	0.0	0.0	0.0	1.5516806641193242E-4
4	0.0	0.0	0.0	0.0	1.5516806641193242E-4
5	0.0	0.0	0.0	0.0	1.5516806641193242E-4
6	0.0	0.0	0.0	0.0	2.3275209961789864E-4
7	0.0	0.0	0.0	0.0	2.3275209961789864E-4
8	0.0	0.0	0.0	0.0	2.3275209961789864E-4
9	0.0	0.0	0.0	0.0	2.3275209961789864E-4
10	0.0	0.0	0.0	0.0	3.1033613282386484E-4
11	0.0	0.0	0.0	0.0	3.1033613282386484E-4
12	0.0	0.0	0.0	0.0	6.206722656477297E-4
13	0.0	0.0	0.0	0.0	9.310083984715946E-4
14	0.0	0.0	0.0	0.0	0.0011637604980894933
15	0.0	0.0	0.0	7.758403320596621E-5	0.0013965125977073918
16	0.0	0.0	0.0	7.758403320596621E-5	0.0013965125977073918
17	0.0	0.0	0.0	7.758403320596621E-5	0.0013965125977073918
18	0.0	0.0	0.0	3.1033613282386484E-4	0.001474096630913358
19	0.0	0.0	0.0	3.8792016602983104E-4	0.001474096630913358
20	0.0	0.0	0.0	6.206722656477297E-4	0.0017068487305312568
21	0.0	0.0	0.0	8.534243652656284E-4	0.0017068487305312568
22	0.0	0.0	0.0	0.0011637604980894933	0.0017068487305312568
23	0.0	0.0	0.0	0.0018620167969431892	0.0017844327637372228
24	0.0	0.0	0.0	0.0036464495606804118	0.0017844327637372228
25	0.0	0.0	0.0	0.005430882324417635	0.0019396008301491552
26	0.0	0.0	0.0	0.0072928991213608236	0.002094768896561088
27	0.0	0.0	0.0	0.009853172217157709	0.002094768896561088
28	0.0	0.0	0.0	0.01349962177783812	0.002172352929767054
29	0.0	0.0	0.0	0.021568361231258607	0.002172352929767054
30	0.0	0.0	0.0	0.03530073510871463	0.002172352929767054
31	7.758403320596621E-5	0.0	0.0	0.0640844114281281	0.00224993696297302
32	7.758403320596621E-5	0.0	0.0	0.10023857090210835	0.00224993696297302
33	7.758403320596621E-5	0.0	0.0	0.13654789844250054	0.00224993696297302
34	7.758403320596621E-5	0.0	0.0	0.1823224780340206	0.0024051050293849526
35	7.758403320596621E-5	0.0	0.0	0.2353899567469015	0.0024051050293849526
36	7.758403320596621E-5	0.0	0.0	0.31374983028492737	0.0024051050293849526
37	7.758403320596621E-5	0.0	0.0	0.41918653141183543	0.0024051050293849526
38	7.758403320596621E-5	0.0	0.0	0.5607773920127238	0.0024051050293849526
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	315	0.0	21.658949	1
CGCAATA	330	0.0	21.33309	36
CAATACG	325	0.0	20.307459	38
TAGCGGC	330	0.0	19.999771	30
CTAGCGG	330	0.0	19.999771	29
GTCTTAA	145	2.9467628E-10	19.731565	1
CCCTATA	125	2.2864697E-8	19.36128	2
TAGGACG	410	0.0	19.31685	4
GTAGGAC	670	0.0	19.045298	3
TGTAGGA	725	0.0	18.815037	2
TCTAGCG	355	0.0	18.591337	28
GATTTCG	225	0.0	18.577566	41
GCGCAAT	370	0.0	18.43222	35
AAGACGG	455	0.0	18.373417	5
TACGAAT	360	0.0	18.333122	41
CGAATGC	360	0.0	18.333122	43
GACGTGA	290	0.0	18.206686	7
ATACGAA	365	0.0	18.081984	40
ATACACT	455	0.0	17.889906	4
AATACGA	370	0.0	17.837633	39
>>END_MODULE