FastQCFastQC Report
Thu 2 Feb 2017
SRR4062487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865217
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29750.15949886796013546No Hit
GCGCAAGACGGACCAGAGCGAAAGC23360.12524011951424419No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21690.11628673768253237No Hit
GAATAGGACCGCGGTTCTATTTTGT20500.10990678296412695No Hit
GATTAAGAGGGACGGCCGGGGGCAT19880.10658277294277288No Hit
GGGTAGGCACACGCTGAGCCAGTCA19470.10438463728349033No Hit
GAATAACGCCGCCGCATCGCCAGTC19370.10384850663488485No Hit
GAACTACGACGGTATCTGATCGTCT19360.10379489357002429No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19270.10331237598627935No Hit
TATCAACGCAGAGTACTTTTTTTTT18910.10138230565129955No Hit
GTATCTGATCGTCTTCGAACCTCCG18760.1005781096783913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2050.014.8184631
ACGAACG2050.013.903515
CGTTATT2150.013.68989852
CGAACGA2100.013.57392216
TAGATCG500.001500093713.2995875
AACCGCG2300.013.219117
CATTCCG905.3983786E-712.6683149
GTATTAC905.4548946E-712.6574381
TAACGAA2350.012.12923613
GTATCAA23450.012.0232171
GATAACG2450.012.02164711
AGAACCG2900.011.7927375
CGCATCG2500.011.7815313
CGATAAC2600.011.69414210
CCGATAA2600.011.6938299
GCGGCCT658.015984E-411.6938299
ACGGTAT3100.011.6466759
GTCTTAG1552.5465852E-1111.6366761
TATACAC2300.011.5598933
AGCCCCG3700.011.55617616
Produced by FastQC (version 0.11.5)