##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062487_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1865217
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15618665281305	32.0	32.0	32.0	32.0	32.0
2	30.775228833964093	32.0	32.0	32.0	32.0	32.0
3	30.749530483584483	32.0	32.0	32.0	32.0	32.0
4	30.840790106459462	32.0	32.0	32.0	32.0	32.0
5	30.61908721612552	32.0	32.0	32.0	32.0	32.0
6	34.26714371571779	36.0	36.0	36.0	32.0	36.0
7	34.15151052129591	36.0	36.0	36.0	32.0	36.0
8	34.14559807250309	36.0	36.0	36.0	32.0	36.0
9	34.32915741171134	36.0	36.0	36.0	32.0	36.0
10	33.96289547007131	36.0	36.0	36.0	32.0	36.0
11	34.29933728890526	36.0	36.0	36.0	32.0	36.0
12	34.058350851402274	36.0	36.0	36.0	32.0	36.0
13	34.19847449385246	36.0	36.0	36.0	32.0	36.0
14	34.091519646239554	36.0	36.0	36.0	32.0	36.0
15	34.02559273264183	36.0	36.0	36.0	32.0	36.0
16	34.02819243015692	36.0	36.0	36.0	32.0	36.0
17	33.954724838986564	36.0	36.0	36.0	32.0	36.0
18	33.95915971171183	36.0	36.0	36.0	32.0	36.0
19	33.94420327500768	36.0	36.0	36.0	32.0	36.0
20	33.949421970741206	36.0	36.0	36.0	32.0	36.0
21	33.937596537024916	36.0	36.0	36.0	32.0	36.0
22	33.885548437527646	36.0	36.0	36.0	32.0	36.0
23	33.85833015675924	36.0	36.0	36.0	32.0	36.0
24	33.81538501954464	36.0	36.0	36.0	32.0	36.0
25	33.363713176536564	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	74.0
5	180.0
6	333.0
7	93.0
8	282.0
9	325.0
10	222.0
11	63.0
12	141.0
13	101.0
14	223.0
15	286.0
16	443.0
17	652.0
18	993.0
19	1342.0
20	2361.0
21	3566.0
22	5573.0
23	8322.0
24	11948.0
25	17018.0
26	23642.0
27	30661.0
28	41345.0
29	54808.0
30	70328.0
31	95970.0
32	136004.0
33	193122.0
34	419972.0
35	744813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.149900941790236	17.744421776184744	11.012913759037888	26.092763522987134
2	17.27903724943115	20.04550825775431	36.23128574768009	26.444168745134455
3	19.10569781338349	23.73296288333345	28.256910148896665	28.904429154386396
4	12.62428650710879	15.301094670352208	36.10605138489339	35.96856743764562
5	14.785694211737852	36.21031980143006	33.62677604889592	15.377209937936168
6	34.11077575641198	35.54880197558305	16.751959518569635	13.58846274943533
7	30.14989240516176	30.294049637943772	20.96787606018873	18.588181896705734
8	28.160276543146278	32.31808257122451	19.688781640059265	19.83285924556995
9	27.205334263520133	14.11578142399077	18.866066517300133	39.81281779518896
10	15.879977606240194	26.436713742586633	31.649704055621548	26.03360459555163
11	37.576846750951844	21.339845546954553	22.097027259293135	18.986280442800467
12	24.44253210167961	24.018911793458393	28.547102058082846	22.99145404677915
13	29.037596487272804	19.445231730490516	25.476075450631797	26.041096331604884
14	23.284967362363652	19.902462853216523	25.031080906982737	31.781488877437088
15	25.139271392832015	27.213139039519852	22.151759792473683	25.495829775174457
16	25.85194961754787	25.88726692855866	23.53276163659775	24.728021817295716
17	23.944027144730562	25.83478581547187	25.151390061640356	25.06979697815721
18	24.85485333409206	24.897639054934825	26.10911378800722	24.13839382296589
19	25.434813185208565	25.07346649101277	25.337591544562514	24.15412877921615
20	25.460940066518802	24.51758840365617	24.866286048014636	25.155185481810392
21	26.7899850061763	24.324083473671156	24.361339877719544	24.524591642433005
22	25.836667762034548	24.35614920199228	25.004778810860035	24.802404225113133
23	24.450570837236896	24.167044719386453	25.548382247539074	25.834002195837574
24	24.629885733796897	25.169595440518655	25.223335559530735	24.97718326615371
25	24.614694152327136	24.793140860699367	25.3356210015241	25.256543985449397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	112.0
2	427.5
3	743.0
4	743.0
5	743.0
6	1902.5
7	3062.0
8	3062.0
9	3062.0
10	3464.5
11	3867.0
12	3867.0
13	3867.0
14	4815.0
15	5763.0
16	5763.0
17	5763.0
18	9368.0
19	12973.0
20	12973.0
21	12973.0
22	20706.0
23	28439.0
24	28439.0
25	28439.0
26	41907.5
27	55376.0
28	55376.0
29	55376.0
30	68372.5
31	81369.0
32	81369.0
33	81369.0
34	100551.5
35	119734.0
36	119734.0
37	119734.0
38	136120.0
39	152506.0
40	152506.0
41	152506.0
42	172706.5
43	192907.0
44	192907.0
45	192907.0
46	215505.5
47	238104.0
48	238104.0
49	238104.0
50	241736.0
51	245368.0
52	245368.0
53	245368.0
54	229169.5
55	212971.0
56	212971.0
57	212971.0
58	195650.0
59	178329.0
60	178329.0
61	178329.0
62	158306.5
63	138284.0
64	138284.0
65	138284.0
66	115206.0
67	92128.0
68	92128.0
69	92128.0
70	69628.5
71	47129.0
72	47129.0
73	47129.0
74	37020.0
75	26911.0
76	26911.0
77	26911.0
78	21980.5
79	17050.0
80	17050.0
81	17050.0
82	11979.0
83	6908.0
84	6908.0
85	6908.0
86	5035.0
87	3162.0
88	3162.0
89	3162.0
90	2106.5
91	1051.0
92	1051.0
93	1051.0
94	633.5
95	216.0
96	216.0
97	216.0
98	485.5
99	755.0
100	755.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00814918585880356
2	0.0032703969564935337
3	0.005254080356333874
4	0.014582753642069528
5	0.03672494942947657
6	0.04589278352063057
7	0.06862472302150367
8	0.08781820024158046
9	0.09607461223010513
10	0.11784151656348832
11	0.1209510743254002
12	0.13210259181639455
13	0.12207694868747174
14	0.12529373257910473
15	0.11408860202324984
16	0.11301634072603883
17	0.12406063208731208
18	0.13113755664890467
19	0.132048978751534
20	0.12250585320635614
21	0.13135200890834686
22	0.1516713604904952
23	0.13950119476715042
24	0.13665970232954128
25	0.13274594859472116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1865217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.618270144819554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98534381933526	39.47483200783746
2	13.261841957416976	13.425829976368703
3	3.9519814434033886	6.001273929285199
4	1.5596092361130356	3.157788865357012
5	0.8257283795994753	2.0898471092405178
6	0.5035970559270866	1.5294727092631843
7	0.31867138392787187	1.1291415939359168
8	0.23511857331218752	0.9521036367984697
9	0.17035665162069386	0.7760843111432875
>10	0.9790571610557735	9.195518057365764
>50	0.09654147832055317	3.4496228824033435
>100	0.09246829114005051	10.03644113612805
>500	0.014683624314746346	4.9645436687895295
>1k	0.005000944512993321	3.817500116083721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2975	0.15949886796013546	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2336	0.12524011951424419	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2169	0.11628673768253237	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2050	0.10990678296412695	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1988	0.10658277294277288	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1947	0.10438463728349033	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1937	0.10384850663488485	No Hit
GAACTACGACGGTATCTGATCGTCT	1936	0.10379489357002429	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1927	0.10331237598627935	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1891	0.10138230565129955	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1876	0.1005781096783913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0722612972109947E-4
2	0.0	0.0	0.0	0.0	1.0722612972109947E-4
3	0.0	0.0	0.0	0.0	1.0722612972109947E-4
4	0.0	0.0	0.0	0.0	1.0722612972109947E-4
5	0.0	0.0	0.0	0.0	1.608391945816492E-4
6	0.0	0.0	0.0	0.0	2.1445225944219894E-4
7	0.0	0.0	0.0	5.3613064860549735E-5	2.1445225944219894E-4
8	0.0	0.0	0.0	5.3613064860549735E-5	2.1445225944219894E-4
9	0.0	0.0	0.0	5.3613064860549735E-5	2.1445225944219894E-4
10	0.0	0.0	0.0	5.3613064860549735E-5	2.1445225944219894E-4
11	0.0	0.0	0.0	5.3613064860549735E-5	2.1445225944219894E-4
12	0.0	0.0	0.0	5.3613064860549735E-5	5.361306486054973E-4
13	0.0	0.0	0.0	5.3613064860549735E-5	7.505829080476964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	205	0.0	14.818463	1
ACGAACG	205	0.0	13.9035	15
CGTTATT	215	0.0	13.6898985	2
CGAACGA	210	0.0	13.573922	16
TAGATCG	50	0.0015000937	13.299587	5
AACCGCG	230	0.0	13.21911	7
CATTCCG	90	5.3983786E-7	12.668314	9
GTATTAC	90	5.4548946E-7	12.657438	1
TAACGAA	235	0.0	12.129236	13
GTATCAA	2345	0.0	12.023217	1
GATAACG	245	0.0	12.021647	11
AGAACCG	290	0.0	11.792737	5
CGCATCG	250	0.0	11.78153	13
CGATAAC	260	0.0	11.694142	10
CCGATAA	260	0.0	11.693829	9
GCGGCCT	65	8.015984E-4	11.693829	9
ACGGTAT	310	0.0	11.646675	9
GTCTTAG	155	2.5465852E-11	11.636676	1
TATACAC	230	0.0	11.559893	3
AGCCCCG	370	0.0	11.556176	16
>>END_MODULE