FastQCFastQC Report
Thu 2 Feb 2017
SRR4062487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865217
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22300.11955713463902592No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21940.11762706430404613No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20470.1097459437695453No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20060.10754780811026278No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19950.10695806439679674No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19730.10577857696986463No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19530.10470631567265365No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19290.10341960211600043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28300.021.304781
GTATAGG1900.019.6883011
CGCAATA6800.019.41163436
TATACGG808.992369E-519.249875
CGAATGC7050.018.72327843
ACCGTAT600.0074138618.333218
GGCGTTA6250.017.9518842
ATACGAA7500.017.89321340
GTATTAC1509.580617E-917.6036571
CGTACAC1002.484328E-517.5998823
TAGCGGC7650.017.25478630
AATACGA7800.017.20501339
CTAGCGG7800.017.20501329
CGGCGTT6650.017.20289241
TACGAAT8200.016.90232541
AACGCAG34750.016.7768676
TCTAGCG8000.016.77488928
CGGTCCA8200.016.63403510
GCAATAC8100.016.56779137
TTAACGG2700.016.29618635
Produced by FastQC (version 0.11.5)