##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1865217
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29651456104035	32.0	32.0	32.0	32.0	32.0
2	31.37786166435326	32.0	32.0	32.0	32.0	32.0
3	31.47127867695823	32.0	32.0	32.0	32.0	32.0
4	31.571489001011678	32.0	32.0	32.0	32.0	32.0
5	31.50775754242	32.0	32.0	32.0	32.0	32.0
6	35.08311258153877	36.0	36.0	36.0	36.0	36.0
7	35.09826309753771	36.0	36.0	36.0	36.0	36.0
8	35.039717094579345	36.0	36.0	36.0	36.0	36.0
9	35.182838779616525	36.0	36.0	36.0	36.0	36.0
10	35.039459751868016	36.0	36.0	36.0	36.0	36.0
11	35.18518703185742	36.0	36.0	36.0	36.0	36.0
12	35.08797582265227	36.0	36.0	36.0	36.0	36.0
13	35.14470434271187	36.0	36.0	36.0	36.0	36.0
14	35.10372466045506	36.0	36.0	36.0	36.0	36.0
15	35.05852938290826	36.0	36.0	36.0	36.0	36.0
16	35.07989633377779	36.0	36.0	36.0	36.0	36.0
17	35.0427333656084	36.0	36.0	36.0	36.0	36.0
18	35.05897973265309	36.0	36.0	36.0	36.0	36.0
19	35.04127669863614	36.0	36.0	36.0	36.0	36.0
20	35.03895203614378	36.0	36.0	36.0	36.0	36.0
21	35.03798807323759	36.0	36.0	36.0	36.0	36.0
22	35.02314744075354	36.0	36.0	36.0	36.0	36.0
23	34.984807665810465	36.0	36.0	36.0	36.0	36.0
24	34.958679874781325	36.0	36.0	36.0	32.0	36.0
25	34.934020009468064	36.0	36.0	36.0	32.0	36.0
26	34.899496948612416	36.0	36.0	36.0	32.0	36.0
27	34.88215365826068	36.0	36.0	36.0	32.0	36.0
28	34.87231566085876	36.0	36.0	36.0	32.0	36.0
29	34.84707034087723	36.0	36.0	36.0	32.0	36.0
30	34.82848054676748	36.0	36.0	36.0	32.0	36.0
31	34.83650374192386	36.0	36.0	36.0	32.0	36.0
32	34.80567515736775	36.0	36.0	36.0	32.0	36.0
33	34.79036004925968	36.0	36.0	36.0	32.0	36.0
34	34.77839039639892	36.0	36.0	36.0	32.0	36.0
35	34.753950344651585	36.0	36.0	36.0	32.0	36.0
36	34.737445562634264	36.0	36.0	36.0	32.0	36.0
37	34.7335125081961	36.0	36.0	36.0	32.0	36.0
38	34.69992231466902	36.0	36.0	36.0	32.0	36.0
39	34.70335569534269	36.0	36.0	36.0	32.0	36.0
40	34.701065345211845	36.0	36.0	36.0	32.0	36.0
41	34.66919613106679	36.0	36.0	36.0	32.0	36.0
42	34.65522617475607	36.0	36.0	36.0	32.0	36.0
43	34.64462472731055	36.0	36.0	36.0	32.0	36.0
44	34.61349751798316	36.0	36.0	36.0	32.0	36.0
45	34.580275110081026	36.0	36.0	36.0	32.0	36.0
46	34.581781101072956	36.0	36.0	36.0	32.0	36.0
47	34.56785564360608	36.0	36.0	36.0	32.0	36.0
48	34.52212316314938	36.0	36.0	36.0	32.0	36.0
49	34.515362019539815	36.0	36.0	36.0	32.0	36.0
50	33.9979031930333	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	42.0
22	107.0
23	375.0
24	1045.0
25	2454.0
26	5197.0
27	10135.0
28	17113.0
29	26938.0
30	40761.0
31	60092.0
32	90004.0
33	150736.0
34	335027.0
35	1125183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80222057068216	18.31445322763477	11.422544379777	26.46078182190607
2	16.765729935264844	20.49757176144607	35.949243433997914	26.78745486929117
3	18.54363149065436	24.04588030995051	28.638629295180518	28.771858904214614
4	12.481664321987374	15.83521694002196	36.16229970148229	35.52081903650837
5	14.530059789321987	36.605894437963215	33.38455170875652	15.479494063958278
6	34.34262905487094	35.82108036961283	16.54309603017379	13.293194545342443
7	30.239484199425586	30.403593790963733	20.86609761759624	18.490824392014442
8	28.3213695779097	32.4740767428133	19.460738348406647	19.743815330870348
9	27.352956787333593	13.950280315909625	18.703239354991936	39.993523541764844
10	15.945919429213868	26.557875035451637	31.467491450056485	26.028714085278015
11	37.783646621277846	21.233615177215306	22.068263370964345	18.914474830542506
12	24.520364118491305	23.952655374682948	28.487302013653103	23.039678493172644
13	29.21638608269172	19.333943450011446	25.426317688504874	26.02335277879196
14	23.366664575757138	19.840854978267945	24.882949276143204	31.909531169831713
15	25.29700297606123	27.303418315402446	21.91879014613313	25.480788562403195
16	26.09380034601872	25.88240403127357	23.37063194255682	24.65316368015089
17	24.208872211651514	25.89891685525062	24.859359527604564	25.0328514054933
18	25.133536741301416	24.877212678203126	25.85693782546481	24.132312755030647
19	25.59455548603728	25.15739455516436	25.071399199128034	24.176650759670324
20	25.694543852002205	24.51087460601099	24.633166006957904	25.1614155350289
21	27.038623388056187	24.30896780374616	24.17718689031893	24.475221917878724
22	25.946096352327906	24.39952027029563	24.831159055487912	24.823224321888553
23	24.60737812275998	24.216699719121156	25.406641693701054	25.769280464417815
24	24.81218003052728	25.172674278649616	24.998217365593387	25.01692832522972
25	24.85528493467516	24.67482335835455	25.2180845445865	25.251807162383788
26	24.65616601178308	25.482665019673313	25.490653366337533	24.37051560220607
27	25.44229438183332	24.93291665259324	24.682865318083632	24.94192364748981
28	24.583627535026757	24.75567186016426	25.674117274290335	24.98658333051865
29	24.633273233087625	24.88369985905125	25.39945754300974	25.083569364851382
30	24.461282521014983	25.162273344066673	25.693900495223883	24.682543639694472
31	25.11954373137281	24.883753472116112	24.664261584577023	25.332441211934054
32	24.883324567597228	24.9294318033773	24.55671377646676	25.63052985255871
33	24.549422399645728	24.571135690914247	25.10174419383911	25.777697715600915
34	25.292445865548085	24.723289568988488	25.40514052788496	24.57912403757847
35	25.827182574467205	24.592741756053048	25.21492137375973	24.365154295720014
36	24.523420063188357	25.200660298506822	24.948142763013635	25.327776875291185
37	25.67690515366309	24.946534371067816	24.63675808230356	24.739802392965537
38	24.730420106614943	24.602392107727947	25.315285031178682	25.351902754478434
39	25.28912185552673	24.67948769499742	24.594135695739425	25.437254753736426
40	25.518264094740715	24.75352733756984	25.265853785377253	24.462354782312193
41	24.38804707441547	25.22628734351017	25.569142893293378	24.816522688780985
42	25.648436616222135	25.345683638954608	24.933881687760728	24.07199805706253
43	24.79754366382035	24.28371605019684	25.37238294525516	25.54635734072765
44	24.451096038691478	24.904180049827982	25.074615983019672	25.57010792846087
45	24.697769750114865	25.048720872692027	25.24505191621136	25.008457460981752
46	24.557999542143445	24.791860232584696	25.132089471819036	25.518050753452826
47	24.749787692149326	24.539517139033766	25.621590207246776	25.089104961570136
48	25.530219808204624	25.168438846525632	24.655737107264194	24.64560423800555
49	24.326820954344722	25.209881745662837	25.12152741477265	25.34176988521979
50	24.300979457081937	25.404711623366076	25.045236023476086	25.249072896075898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.0
2	11.0
3	43.5
4	76.0
5	104.5
6	133.0
7	133.0
8	133.0
9	195.5
10	258.0
11	375.5
12	493.0
13	883.5
14	1274.0
15	1779.5
16	2285.0
17	2923.0
18	3561.0
19	4148.5
20	4736.0
21	5876.0
22	7016.0
23	7963.5
24	8911.0
25	11182.0
26	13453.0
27	16973.5
28	20494.0
29	23244.5
30	25995.0
31	27908.5
32	29822.0
33	34355.0
34	38888.0
35	45116.0
36	51344.0
37	62119.0
38	72894.0
39	78625.0
40	84356.0
41	92161.0
42	99966.0
43	100733.0
44	101500.0
45	110134.5
46	118769.0
47	126644.0
48	134519.0
49	138830.5
50	143142.0
51	137425.5
52	131709.0
53	129756.0
54	127803.0
55	129358.0
56	130913.0
57	128315.5
58	125718.0
59	115007.5
60	104297.0
61	91470.5
62	78644.0
63	68729.0
64	58814.0
65	50408.0
66	42002.0
67	37002.5
68	32003.0
69	29213.5
70	26424.0
71	20255.0
72	14086.0
73	12725.0
74	11364.0
75	8314.5
76	5265.0
77	4724.0
78	4183.0
79	3673.5
80	3164.0
81	2523.0
82	1882.0
83	1632.5
84	1383.0
85	1150.0
86	917.0
87	674.0
88	431.0
89	281.5
90	132.0
91	82.0
92	32.0
93	23.5
94	15.0
95	9.5
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015601401874419975
2	0.004235432123983429
3	8.578090377687959E-4
4	5.361306486054974E-5
5	5.361306486054974E-5
6	0.0011794874269320942
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.1445225944219897E-4
47	5.361306486054974E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1865217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.78450651000605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00241723490673	41.17319100332143
2	13.549741935198742	14.304328827747998
3	3.878925401577776	6.142414893342299
4	1.5337990058851554	3.2384329444474313
5	0.7800769582217462	2.058798863978074
6	0.4516502075356668	1.4304079991947192
7	0.3229842232607366	1.1933993984734919
8	0.2249935715662688	0.9500939714439383
9	0.1738582671905745	0.8259320552705327
>10	0.8968870605760747	8.690463161659816
>50	0.08523621575325052	3.1462665195475
>100	0.08370980364964836	9.569597891213096
>500	0.011636968008057495	4.1325124346475075
>1k	0.004083146669493858	3.144160035712241
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2230	0.11955713463902592	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2194	0.11762706430404613	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2047	0.1097459437695453	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2006	0.10754780811026278	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1995	0.10695806439679674	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1973	0.10577857696986463	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1953	0.10470631567265365	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1929	0.10341960211600043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.3613064860549735E-5	0.0
6	0.0	0.0	0.0	5.3613064860549735E-5	1.608391945816492E-4
7	0.0	0.0	0.0	5.3613064860549735E-5	1.608391945816492E-4
8	0.0	0.0	0.0	5.3613064860549735E-5	1.608391945816492E-4
9	0.0	0.0	0.0	1.0722612972109947E-4	1.608391945816492E-4
10	0.0	0.0	0.0	1.0722612972109947E-4	1.608391945816492E-4
11	0.0	0.0	0.0	1.0722612972109947E-4	1.608391945816492E-4
12	0.0	0.0	0.0	1.0722612972109947E-4	3.752914540238482E-4
13	0.0	0.0	0.0	1.0722612972109947E-4	4.8251758374494763E-4
14	0.0	0.0	0.0	1.0722612972109947E-4	4.8251758374494763E-4
15	0.0	0.0	0.0	1.0722612972109947E-4	4.8251758374494763E-4
16	0.0	0.0	0.0	1.0722612972109947E-4	4.8251758374494763E-4
17	0.0	0.0	0.0	1.0722612972109947E-4	4.8251758374494763E-4
18	0.0	0.0	0.0	1.0722612972109947E-4	5.361306486054973E-4
19	0.0	0.0	0.0	1.0722612972109947E-4	5.361306486054973E-4
20	0.0	0.0	0.0	2.1445225944219894E-4	6.433567783265968E-4
21	0.0	0.0	0.0	2.1445225944219894E-4	6.969698431871466E-4
22	0.0	0.0	0.0	3.216783891632984E-4	8.041959729082461E-4
23	0.0	0.0	0.0	3.752914540238482E-4	8.041959729082461E-4
24	0.0	0.0	0.0	7.505829080476964E-4	8.041959729082461E-4
25	0.0	0.0	0.0	9.114221026293455E-4	8.041959729082461E-4
26	0.0	0.0	0.0	9.650351674898953E-4	9.114221026293455E-4
27	0.0	0.0	0.0	0.0016083919458164922	9.114221026293455E-4
28	0.0	0.0	0.0	0.004557110513146728	9.114221026293455E-4
29	0.0	0.0	0.0	0.009864803934341151	9.650351674898953E-4
30	0.0	0.0	0.0	0.02262471337115199	9.650351674898953E-4
31	0.0	0.0	0.0	0.05227273823903599	0.0010722612972109947
32	0.0	0.0	0.0	0.08481586860938968	0.0011794874269320942
33	0.0	0.0	0.0	0.12406063208731209	0.0011794874269320942
34	0.0	0.0	0.0	0.16941728495933717	0.0011794874269320942
35	0.0	0.0	0.0	0.22024247044713832	0.0011794874269320942
36	0.0	0.0	0.0	0.29744528384632996	0.0011794874269320942
37	0.0	0.0	0.0	0.4075665190698991	0.0011794874269320942
38	0.0	0.0	0.0	0.558916201171231	0.0011794874269320942
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2830	0.0	21.30478	1
GTATAGG	190	0.0	19.688301	1
CGCAATA	680	0.0	19.411634	36
TATACGG	80	8.992369E-5	19.24987	5
CGAATGC	705	0.0	18.723278	43
ACCGTAT	60	0.00741386	18.33321	8
GGCGTTA	625	0.0	17.95188	42
ATACGAA	750	0.0	17.893213	40
GTATTAC	150	9.580617E-9	17.603657	1
CGTACAC	100	2.484328E-5	17.599882	3
TAGCGGC	765	0.0	17.254786	30
AATACGA	780	0.0	17.205013	39
CTAGCGG	780	0.0	17.205013	29
CGGCGTT	665	0.0	17.202892	41
TACGAAT	820	0.0	16.902325	41
AACGCAG	3475	0.0	16.776867	6
TCTAGCG	800	0.0	16.774889	28
CGGTCCA	820	0.0	16.634035	10
GCAATAC	810	0.0	16.567791	37
TTAACGG	270	0.0	16.296186	35
>>END_MODULE