FastQCFastQC Report
Thu 2 Feb 2017
SRR4062486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130264
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT57680.5103232519128275No Hit
GTATCAACGCAGAGTACTTTTTTTT57280.5067842557137093No Hit
GTCCTACAGTGGACATTTCTAAATT57020.5044839081842827No Hit
CTGTAGGACGTGGAATATGGCAAGA52840.46750139790349865No Hit
GTCCTACAGTGTGCATTTCTCATTT45810.40530353970399835No Hit
CTTTAGGACGTGAAATATGGCGAGG45400.40167606859990235No Hit
TATCAACGCAGAGTACTTTTTTTTT36230.32054458073512027No Hit
TTGTAGAACAGTGTATATCAATGAG30380.2687867613230183No Hit
GATATACACTGTTCTACAAATCCCG30220.2673711628433711No Hit
CTGTAGGACCTGGAATATGGCGAGA29430.26038164535011293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28990.256488749531083No Hit
GTATTAGAGGCACTGCCTGCCCAGT27940.24719888450839803No Hit
GTGTATATCAATGAGTTACAATGAA27480.24312903887941223No Hit
CCATAGGGTCTTCTCGTCTTATTAT27220.24082869134998547No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23370.20676585293347396No Hit
CTGAAGGACCTGGAATATGGCGAGA22320.19747598791078896No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22180.19623733924109765No Hit
GTGTATATCAATGAGTTACAATGAG21420.1895132464627733No Hit
CCATTGGGATGTCCTGATCCAACAT21350.18889392212792763No Hit
CTCTAATACTTGTAATGCTAGAGGT21040.1861512000736111No Hit
GTACATGGGGTGGTATCAACGCAAA20860.184558651784008No Hit
ATTTAGAAATGTCCACTGTAGGACG20580.1820813544446253No Hit
GGTATCAACGCAGAGTACTTTTTTT20310.17969253201022062No Hit
GTCCTTCAGTGTGCATTTCTCATTT19580.1732338639468301No Hit
GTTCTACAGTGTGGTTTTTATCATT18440.16314772477934358No Hit
GATATATTTTGATCAACGGACCAAG18250.1614667015847625No Hit
CTTCTACACCATTGGGATGTCCTGA17900.15837007991053417No Hit
GTCAGGATACCGCGGCCGTTAAACT17480.1546541339014602No Hit
GATATACACTGTTCTACAATGCCGG17130.15155751222723188No Hit
ATCCTGACCGTGCAAAGGTAGCATA16810.14872631526793742No Hit
GAATATGGCAAGAAAACTGAAAATC16700.14775309131317993No Hit
GGTCAGGATACCGCGGCCGTTAAAC15340.13572050423617843No Hit
CCTCTAGCATTACAAGTATTAGAGG15320.13554355442622254No Hit
GTTCATGCTAGTCCCTAATTAAGGA15240.13483575518639893No Hit
TTTCTAAATTTTCCACCTTTTTCAG15140.13395100613661942No Hit
ATCTATAACTTTATAGATGCAACAC14350.12696148864336118No Hit
CTGTTAGTATGAGTAACAAGAATTC14200.1256343650686919No Hit
CTGTAGAACATATTAGATGAGTGAG14070.12448419130397854No Hit
GTTCTACAAATCCCGTTTCCAACGA14060.12439571639900057No Hit
GTATCCTGACCGTGCAAAGGTAGCA14020.12404181677908878No Hit
ATCGTAAATAGATAGAAACCGACCT13740.12156451943970611No Hit
GTTATATAATTTAAGCTCCATAGGG13560.11997197115010298No Hit
GTACATGGGAGAAATCGTAAATAGA13430.1188217973853896No Hit
GGAATATGGCGAGAAAACTGAAAAT13390.11846789776547781No Hit
CTGTAGGACATGGAATATGGCAAGA13130.11616755023605105No Hit
GCCTAAAGGAAAGATCCAAAAAGAT13020.11519432628129359No Hit
GTATCAACGCAGAGTACATGGGAGA12690.11227465441702114No Hit
TCCCAATGGTGTAGAAGCTATTAAT12420.10988583198261645No Hit
TGCTAGAGGTGATGTTTTTGGTAAA12280.10864718331292512No Hit
GTTATAGATTAACCCAATTTTAAGT12200.10793938407310151No Hit
GTGTAGAAGCTATTAATGGTTCGTT12100.10705463502332199No Hit
ATAAATAATCCACCTATAACTTCTC11810.10448886277896138No Hit
CCTATAACTTCTCTGTTAACCCAAC11690.10342716391922595No Hit
ACCTATAACTTCTCTGTTAACCCAA11660.1031617392042921No Hit
ATGTTCTACAGTGTGGTTTTTATCA11640.1029847893943362No Hit
CACTATAAATAATCCACCTATAACT11630.10289631448935824No Hit
AGCATGAACGGCTAAACGAGGGTCC11390.10077291676988738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT250.00602815219.00159610
ACGGTGC250.00602815219.0015968
AGGACTC402.7613074E-416.6241865
TCGTCCC551.125919E-515.54813813
CACACCG456.762811E-414.7770535
CTAGACA602.5714378E-514.2474084
TAGGACC12150.014.1496894
TTGTAGA6750.013.9227791
AATGTCC5900.013.3655298
AGTGGTC500.001498155213.3011178
AAGCGTT1500.013.30052711
TAGCACC500.001501159913.2975814
TAGAAAT5950.013.2496914
ACTGTTC10050.013.2349428
AAATGTC5600.013.23325357
CGGACCA2600.013.156717
GTCCCCA655.4410884E-513.156116515
GTACTAA802.0150892E-613.0526071
ACGGACC2550.013.04204516
CTAGGAC2550.013.0328033
Produced by FastQC (version 0.11.5)