##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062486_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1130264
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064976854964858	32.0	32.0	32.0	32.0	32.0
2	30.801387109560245	32.0	32.0	32.0	32.0	32.0
3	30.807364474140556	32.0	32.0	32.0	32.0	32.0
4	30.84702511979502	32.0	32.0	32.0	32.0	32.0
5	30.73330124643446	32.0	32.0	32.0	32.0	32.0
6	34.33905706985271	36.0	36.0	36.0	32.0	36.0
7	34.15230689467239	36.0	36.0	36.0	32.0	36.0
8	34.127419788651146	36.0	36.0	36.0	32.0	36.0
9	34.259724276806125	36.0	36.0	36.0	32.0	36.0
10	33.98803553859983	36.0	36.0	36.0	32.0	36.0
11	34.34146181777001	36.0	36.0	36.0	32.0	36.0
12	34.10729174776866	36.0	36.0	36.0	32.0	36.0
13	34.235497193576016	36.0	36.0	36.0	32.0	36.0
14	34.15011891027229	36.0	36.0	36.0	32.0	36.0
15	34.10761822016803	36.0	36.0	36.0	32.0	36.0
16	34.127239299844994	36.0	36.0	36.0	32.0	36.0
17	34.08399453578987	36.0	36.0	36.0	32.0	36.0
18	34.1555079167345	36.0	36.0	36.0	32.0	36.0
19	34.04195391519149	36.0	36.0	36.0	32.0	36.0
20	33.98891763340246	36.0	36.0	36.0	32.0	36.0
21	33.94132698201482	36.0	36.0	36.0	32.0	36.0
22	33.89396017213677	36.0	36.0	36.0	32.0	36.0
23	33.95292073356313	36.0	36.0	36.0	32.0	36.0
24	33.90308193484	36.0	36.0	36.0	32.0	36.0
25	33.541880481020364	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	26.0
5	120.0
6	190.0
7	52.0
8	165.0
9	218.0
10	136.0
11	52.0
12	82.0
13	58.0
14	264.0
15	304.0
16	464.0
17	564.0
18	776.0
19	1121.0
20	1798.0
21	2539.0
22	3905.0
23	5579.0
24	7472.0
25	10243.0
26	14072.0
27	17643.0
28	23400.0
29	30547.0
30	39539.0
31	53324.0
32	74988.0
33	108865.0
34	245603.0
35	486152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25287059346097	21.319061696191802	12.084054834374035	24.3440128759732
2	12.685105965063778	21.461630410066615	46.0015695906412	19.8516940342284
3	18.77531013626442	27.43523657595054	30.6834278716968	23.106025416088244
4	9.63786550807226	17.94435030373284	42.31279394392556	30.104990244269338
5	9.736943653794413	41.714459691009246	37.53071144342014	11.017885211776202
6	27.152508977546617	42.363840911766445	19.449654485055714	11.033995625631217
7	25.352852121716495	33.906915388088635	22.63848925640322	18.101743233791645
8	22.495176006042943	41.39283471965046	20.571423005160057	15.540566269146542
9	26.512316213262576	15.156903969492419	22.023146368723	36.307633448522004
10	14.417875412449895	29.16447062470935	36.09610913036739	20.32154483247337
11	32.46912924085393	22.828328461334042	28.686420409247944	16.01612188856409
12	23.91715349354766	26.48604016166728	33.66511980636782	15.931686538417242
13	28.48166806481131	23.473239334311888	28.0808272291844	19.9642653716924
14	18.60572655614672	26.54489629231977	28.477666572765457	26.371710578768052
15	20.962647368840624	36.49432668715732	24.482758620689655	18.060267323312402
16	18.82465796620318	29.402860275894565	32.93002014203947	18.842461615862785
17	17.379133417430868	31.166286337930348	31.304834663302767	20.149745581336017
18	17.356456966989818	28.815625462338307	36.57218944818843	17.25572812248344
19	22.71733429721608	27.004929268016102	28.918254238249137	21.35948219651868
20	21.67085822207773	30.620884664753817	30.784501491331497	16.923755621836957
21	21.323735908396273	27.33228499922482	28.007884653717525	23.33609443866138
22	20.963182845243896	32.33582916131319	28.466970891852377	18.234017101590535
23	19.80745240356672	31.64622347287393	30.72353312258119	17.822791000978157
24	22.024317897504776	29.665655488183035	30.98139833226427	17.32862828204792
25	20.130427579559317	30.898720619021592	32.01562083332964	16.955230968089452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	260.0
2	675.0
3	1090.0
4	1090.0
5	1090.0
6	3665.0
7	6240.0
8	6240.0
9	6240.0
10	6247.5
11	6255.0
12	6255.0
13	6255.0
14	6908.0
15	7561.0
16	7561.0
17	7561.0
18	12562.0
19	17563.0
20	17563.0
21	17563.0
22	29603.0
23	41643.0
24	41643.0
25	41643.0
26	67428.5
27	93214.0
28	93214.0
29	93214.0
30	121152.0
31	149090.0
32	149090.0
33	149090.0
34	153330.5
35	157571.0
36	157571.0
37	157571.0
38	150574.5
39	143578.0
40	143578.0
41	143578.0
42	135075.0
43	126572.0
44	126572.0
45	126572.0
46	131858.0
47	137144.0
48	137144.0
49	137144.0
50	119099.5
51	101055.0
52	101055.0
53	101055.0
54	79184.0
55	57313.0
56	57313.0
57	57313.0
58	45245.0
59	33177.0
60	33177.0
61	33177.0
62	26876.0
63	20575.0
64	20575.0
65	20575.0
66	17110.0
67	13645.0
68	13645.0
69	13645.0
70	10939.5
71	8234.0
72	8234.0
73	8234.0
74	6438.5
75	4643.0
76	4643.0
77	4643.0
78	3387.0
79	2131.0
80	2131.0
81	2131.0
82	1498.0
83	865.0
84	865.0
85	865.0
86	551.0
87	237.0
88	237.0
89	237.0
90	154.0
91	71.0
92	71.0
93	71.0
94	63.5
95	56.0
96	56.0
97	56.0
98	268.5
99	481.0
100	481.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00858206578286135
2	0.002919671864272418
3	0.003096621674228322
4	0.014067509891494377
5	0.03468216275135721
6	0.044325927393953975
7	0.06671007835337585
8	0.08962507874266543
9	0.09749934528570317
10	0.11846789776547781
11	0.12067977038992661
12	0.13501270499635482
13	0.1227146932044195
14	0.12545741525873602
15	0.11448652704146997
16	0.11342482818173454
17	0.1252804654487801
18	0.1319160833221265
19	0.13235845784701628
20	0.12448419130397854
21	0.1317391335121706
22	0.1516459871322098
23	0.1422676472045469
24	0.13695915290586977
25	0.13403948104159735
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1130264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.34683423379168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74904382617628	19.88219958664683
2	15.646331663758458	9.18340603406491
3	6.331162630177468	5.5739874064498425
4	3.0418516877850927	3.570748689808343
5	1.7215323535226583	2.5260762303469355
6	1.0798777608955012	1.9014596185055042
7	0.6997409814322649	1.4374627812078367
8	0.5256041468401547	1.2339854215929213
9	0.38553799849898696	1.0182887759499837
>10	2.2397715017623434	12.42288374047387
>50	0.26177924241202255	5.4264312294790065
>100	0.2599630205608338	15.960521607270708
>500	0.03510559998239525	7.180316348627515
>1k	0.021487048265086746	10.68552427633395
>5k	0.0012105379304274224	1.9967082532418496
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	5768	0.5103232519128275	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5728	0.5067842557137093	No Hit
GTCCTACAGTGGACATTTCTAAATT	5702	0.5044839081842827	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5284	0.46750139790349865	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4581	0.40530353970399835	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4540	0.40167606859990235	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3623	0.32054458073512027	No Hit
TTGTAGAACAGTGTATATCAATGAG	3038	0.2687867613230183	No Hit
GATATACACTGTTCTACAAATCCCG	3022	0.2673711628433711	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2943	0.26038164535011293	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2899	0.256488749531083	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2794	0.24719888450839803	No Hit
GTGTATATCAATGAGTTACAATGAA	2748	0.24312903887941223	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2722	0.24082869134998547	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2337	0.20676585293347396	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2232	0.19747598791078896	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2218	0.19623733924109765	No Hit
GTGTATATCAATGAGTTACAATGAG	2142	0.1895132464627733	No Hit
CCATTGGGATGTCCTGATCCAACAT	2135	0.18889392212792763	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2104	0.1861512000736111	No Hit
GTACATGGGGTGGTATCAACGCAAA	2086	0.184558651784008	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2058	0.1820813544446253	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2031	0.17969253201022062	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1958	0.1732338639468301	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1844	0.16314772477934358	No Hit
GATATATTTTGATCAACGGACCAAG	1825	0.1614667015847625	No Hit
CTTCTACACCATTGGGATGTCCTGA	1790	0.15837007991053417	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1748	0.1546541339014602	No Hit
GATATACACTGTTCTACAATGCCGG	1713	0.15155751222723188	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1681	0.14872631526793742	No Hit
GAATATGGCAAGAAAACTGAAAATC	1670	0.14775309131317993	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1534	0.13572050423617843	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1532	0.13554355442622254	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1524	0.13483575518639893	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1514	0.13395100613661942	No Hit
ATCTATAACTTTATAGATGCAACAC	1435	0.12696148864336118	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1420	0.1256343650686919	No Hit
CTGTAGAACATATTAGATGAGTGAG	1407	0.12448419130397854	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1406	0.12439571639900057	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1402	0.12404181677908878	No Hit
ATCGTAAATAGATAGAAACCGACCT	1374	0.12156451943970611	No Hit
GTTATATAATTTAAGCTCCATAGGG	1356	0.11997197115010298	No Hit
GTACATGGGAGAAATCGTAAATAGA	1343	0.1188217973853896	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1339	0.11846789776547781	No Hit
CTGTAGGACATGGAATATGGCAAGA	1313	0.11616755023605105	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1302	0.11519432628129359	No Hit
GTATCAACGCAGAGTACATGGGAGA	1269	0.11227465441702114	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1242	0.10988583198261645	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1228	0.10864718331292512	No Hit
GTTATAGATTAACCCAATTTTAAGT	1220	0.10793938407310151	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1210	0.10705463502332199	No Hit
ATAAATAATCCACCTATAACTTCTC	1181	0.10448886277896138	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1169	0.10342716391922595	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1166	0.1031617392042921	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1164	0.1029847893943362	No Hit
CACTATAAATAATCCACCTATAACT	1163	0.10289631448935824	No Hit
AGCATGAACGGCTAAACGAGGGTCC	1139	0.10077291676988738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.538996199118082E-4	0.0
8	0.0	0.0	0.0	3.538996199118082E-4	0.0
9	0.0	0.0	0.0	4.423745248897603E-4	0.0
10	0.0	0.0	0.0	4.423745248897603E-4	0.0
11	0.0	0.0	0.0	6.193243348456644E-4	0.0
12	0.0	0.0	0.0	6.193243348456644E-4	0.0
13	0.0	0.0	0.0	6.193243348456644E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAT	25	0.006028152	19.001596	10
ACGGTGC	25	0.006028152	19.001596	8
AGGACTC	40	2.7613074E-4	16.624186	5
TCGTCCC	55	1.125919E-5	15.548138	13
CACACCG	45	6.762811E-4	14.777053	5
CTAGACA	60	2.5714378E-5	14.247408	4
TAGGACC	1215	0.0	14.149689	4
TTGTAGA	675	0.0	13.922779	1
AATGTCC	590	0.0	13.365529	8
AGTGGTC	50	0.0014981552	13.301117	8
AAGCGTT	150	0.0	13.300527	11
TAGCACC	50	0.0015011599	13.297581	4
TAGAAAT	595	0.0	13.249691	4
ACTGTTC	1005	0.0	13.234942	8
AAATGTC	560	0.0	13.2332535	7
CGGACCA	260	0.0	13.1567	17
GTCCCCA	65	5.4410884E-5	13.1561165	15
GTACTAA	80	2.0150892E-6	13.052607	1
ACGGACC	255	0.0	13.042045	16
CTAGGAC	255	0.0	13.032803	3
>>END_MODULE