FastQCFastQC Report
Thu 2 Feb 2017
SRR4062486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130264
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28200.2494992320378248No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT26400.2335737491417934No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG26290.23260052518703594No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA21660.19163664418224416No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC21070.18641662478854498No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT20650.182700678779471No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA19830.17544573657127893No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG18390.16270535025445385No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA17290.1529731107068791No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC17190.15208836165709957No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA17180.15199988675212164No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC16480.14580664340366498No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT16280.14403714530410594No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC14570.12890793655287613No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14440.12775776278816275No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA14330.1267845388334053No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC14160.1252804654487801No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC14120.1249265658288683No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC13930.12324554263428722No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT13750.12165299434468407No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA13500.11944112172023527No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT13490.11935264681525733No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC13470.11917569700530142No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT12770.11298245365684477No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC12770.11298245365684477No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA12740.11271702894191092No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC12690.11227465441702114No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC12300.10882413312288103No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC12130.10732005973825584No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11830.10466581258891727No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA11760.10404648825407162No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT11750.10395801334909366No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG11460.10139224110473305No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT11450.1013037661997551No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG11390.10077291676988738No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA11320.10015359243504172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT407.031973E-427.4999166
TAGGACC16000.026.1249224
ATAGGAC3000.024.1999283
TTTCGCC1102.0190782E-1023.99992643
TAAGACC852.3524444E-723.2940464
AGGACCT23550.022.4203135
GTCCTAT2650.022.4170111
TCCTACA35100.022.0635872
ACTGTTC14000.021.9999358
GGACCTG23800.021.9999336
TGTAGGA39750.021.8625372
CCTAGAG2250.021.5110453
CTGTAGG38100.021.4821511
GATATAC16400.021.331091
CCTACAG33950.021.3195253
ATACACT16550.021.1358874
TAGGACA9500.021.073624
GTCCTAC37200.020.9964181
CAGTGAT12600.020.60311137
TTAGGAC20550.020.340573
Produced by FastQC (version 0.11.5)