##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1130264
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177749623096904	32.0	32.0	32.0	32.0	32.0
2	31.4628732756241	32.0	32.0	32.0	32.0	32.0
3	31.55449523297212	32.0	32.0	32.0	32.0	32.0
4	31.65353492635349	32.0	32.0	32.0	32.0	32.0
5	31.613903477417665	32.0	32.0	32.0	32.0	32.0
6	35.194720879369775	36.0	36.0	36.0	36.0	36.0
7	35.217283749637254	36.0	36.0	36.0	36.0	36.0
8	35.165108328673654	36.0	36.0	36.0	36.0	36.0
9	35.28563415272892	36.0	36.0	36.0	36.0	36.0
10	35.19622406800535	36.0	36.0	36.0	36.0	36.0
11	35.290279969989314	36.0	36.0	36.0	36.0	36.0
12	35.22056086011764	36.0	36.0	36.0	36.0	36.0
13	35.25474667865207	36.0	36.0	36.0	36.0	36.0
14	35.22934464868385	36.0	36.0	36.0	36.0	36.0
15	35.197898897956584	36.0	36.0	36.0	36.0	36.0
16	35.22226931053276	36.0	36.0	36.0	36.0	36.0
17	35.21896477283183	36.0	36.0	36.0	36.0	36.0
18	35.22883326373307	36.0	36.0	36.0	36.0	36.0
19	35.183643821266536	36.0	36.0	36.0	36.0	36.0
20	35.14249768195749	36.0	36.0	36.0	36.0	36.0
21	35.12002151709689	36.0	36.0	36.0	36.0	36.0
22	35.11191721580091	36.0	36.0	36.0	36.0	36.0
23	35.10931251459836	36.0	36.0	36.0	36.0	36.0
24	35.092698696941596	36.0	36.0	36.0	36.0	36.0
25	35.07618927967271	36.0	36.0	36.0	36.0	36.0
26	35.042444950914124	36.0	36.0	36.0	36.0	36.0
27	35.00088386430073	36.0	36.0	36.0	36.0	36.0
28	34.976930168527	36.0	36.0	36.0	36.0	36.0
29	34.944673987670136	36.0	36.0	36.0	36.0	36.0
30	34.89638969302747	36.0	36.0	36.0	36.0	36.0
31	34.89544212679515	36.0	36.0	36.0	36.0	36.0
32	34.847647983126066	36.0	36.0	36.0	36.0	36.0
33	34.85075699128699	36.0	36.0	36.0	36.0	36.0
34	34.81607748278278	36.0	36.0	36.0	36.0	36.0
35	34.77921529837277	36.0	36.0	36.0	36.0	36.0
36	34.71687322607816	36.0	36.0	36.0	32.0	36.0
37	34.66430320703836	36.0	36.0	36.0	32.0	36.0
38	34.61955083060241	36.0	36.0	36.0	32.0	36.0
39	34.499902677604524	36.0	36.0	36.0	32.0	36.0
40	34.54715535485515	36.0	36.0	36.0	32.0	36.0
41	34.492143428437956	36.0	36.0	36.0	32.0	36.0
42	34.47862888670257	36.0	36.0	36.0	32.0	36.0
43	34.45235626366937	36.0	36.0	36.0	32.0	36.0
44	34.36782026146104	36.0	36.0	36.0	32.0	36.0
45	34.30587721098787	36.0	36.0	36.0	32.0	36.0
46	34.16032006681625	36.0	36.0	36.0	32.0	36.0
47	34.13052260356872	36.0	36.0	36.0	32.0	36.0
48	34.039691611871206	36.0	36.0	36.0	32.0	36.0
49	33.98335079238125	36.0	36.0	36.0	32.0	36.0
50	33.55803865291649	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	10.0
21	31.0
22	93.0
23	244.0
24	598.0
25	1636.0
26	3600.0
27	7089.0
28	12222.0
29	18653.0
30	26463.0
31	37660.0
32	53522.0
33	85986.0
34	178765.0
35	703689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.693418707669	22.11635969779184	12.488474287706556	24.7017473068326
2	12.236830114022956	22.13524731222754	45.438595094190376	20.189327479559125
3	18.321552847621653	27.69542202273992	31.12790962071525	22.855115508923177
4	9.560438199284768	18.41484540752498	42.55181541978762	29.472900973402627
5	9.503708868016677	41.85818534430894	37.5180488806155	11.120056907058881
6	27.333911961771594	42.362148852608115	19.180824925968295	11.123114259651992
7	25.200307184870084	33.9530410594339	22.856164577479245	17.99048717821677
8	22.485454725621626	41.587098235456494	20.57758187467707	15.349865164244815
9	26.20016208602592	14.905367241635584	22.191010241855	36.703460430483496
10	14.379206981731702	29.244318141602317	36.090240864081316	20.28623401258467
11	32.375975878201906	22.79582469228428	28.815657226984136	16.012542202529676
12	23.81850611892443	26.441079252280886	33.78352314149615	15.956891487298543
13	28.459988108972773	23.347377249916835	28.380095269777676	19.812539371332715
14	18.54301295980408	26.54123284471592	28.587745871760934	26.32800832371906
15	20.876980953122455	36.387251120092294	24.61124126752688	18.12452665925837
16	18.860637868674928	29.35818534430894	32.80773341449431	18.973443372521817
17	17.37691371219467	31.122994273904148	31.23544587813113	20.26464613577005
18	17.41637351981484	28.532714480864644	36.74504363582313	17.30586836349738
19	22.783703630302302	26.769763524273976	29.3615473906981	21.084985454725622
20	21.56770453628533	30.5594091291946	31.001252804654488	16.87163352986559
21	21.157711826617497	27.112515306158564	28.598982184693135	23.130790682530808
22	20.881758597991265	31.96757571682368	28.95721707494886	18.193448610236192
23	19.817493966011483	31.301005782719788	30.858631257830027	18.0228689934387
24	22.09908481558291	29.32695370285172	31.114677632836223	17.459283848729147
25	20.18855771748901	30.57294578965622	32.115417283041836	17.12307920981293
26	16.36405300000708	31.559440980160385	34.21359965459397	17.862906365238565
27	17.88900646220706	31.54422329650418	31.832209112207416	18.73456112908135
28	17.217924307949293	31.56306845126448	30.50216586567386	20.716841375112363
29	19.34450712399935	28.857240432323778	29.60060658394853	22.197645859728347
30	18.318994500399906	29.61281612083549	31.451678545897245	20.616510832867366
31	21.14470601558574	27.813767403013813	31.088842960582657	19.95268362081779
32	17.743642193328284	31.585894976748797	31.45742941472081	19.213033415202112
33	18.2407826843994	31.491669202947275	32.6536986049277	17.61384950772563
34	18.603264370094067	30.74379083116865	33.56472470148567	17.088220097251615
35	18.371814018671746	32.17602259295174	31.496535322721066	17.955628065655457
36	17.26950517755144	30.17640126554504	33.10093924959124	19.453154307312275
37	18.433923401966265	30.064392035842953	29.224499762887255	22.277184799303527
38	19.121196463835	32.55655315926191	29.66828988625666	18.653960490646433
39	20.20589879886469	29.364732487277305	29.85859940686424	20.570769306993764
40	22.189152268850464	29.576541409794526	31.22120141842968	17.013104902925335
41	21.465162121415883	27.310522143499217	33.049004480369184	18.175311254715712
42	19.166407140278732	32.72677887643949	30.6524847292314	17.454329254050382
43	16.60797831303129	32.79410827912771	32.07648832485154	18.521425082989463
44	16.963735905947637	31.903873785239554	31.01762066207541	20.1147696467374
45	17.292951027370595	32.11426710927712	31.35037477969749	19.242407083654793
46	19.602871190156627	28.744371885127624	29.848805101113633	21.803951823602116
47	21.883913846676528	29.527260887721805	28.941910916387677	19.64691434921399
48	18.401895486364246	30.517472024235047	28.858567555898446	22.22206493350226
49	20.058322657361465	29.321999108172957	30.009095220231735	20.610583014233843
50	17.451763481806022	32.51143095772315	28.82468166729189	21.212123893178937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	53.0
2	58.0
3	166.0
4	274.0
5	349.0
6	424.0
7	340.0
8	256.0
9	396.5
10	537.0
11	814.0
12	1091.0
13	2119.0
14	3147.0
15	4359.0
16	5571.0
17	7254.5
18	8938.0
19	10139.0
20	11340.0
21	13272.0
22	15204.0
23	15110.0
24	15016.0
25	19448.5
26	23881.0
27	34022.5
28	44164.0
29	51268.5
30	58373.0
31	62511.5
32	66650.0
33	80477.5
34	94305.0
35	108753.5
36	123202.0
37	127212.5
38	131223.0
39	118670.0
40	106117.0
41	97261.0
42	88405.0
43	73661.5
44	58918.0
45	54576.0
46	50234.0
47	45996.5
48	41759.0
49	35874.0
50	29989.0
51	29705.0
52	29421.0
53	26291.5
54	23162.0
55	21498.5
56	19835.0
57	19597.5
58	19360.0
59	16313.0
60	13266.0
61	12057.0
62	10848.0
63	9912.0
64	8976.0
65	8299.0
66	7622.0
67	6726.5
68	5831.0
69	5140.5
70	4450.0
71	3734.0
72	3018.0
73	2659.0
74	2300.0
75	1887.5
76	1475.0
77	1120.5
78	766.0
79	596.0
80	426.0
81	311.0
82	196.0
83	146.0
84	96.0
85	72.5
86	49.0
87	34.5
88	20.0
89	16.5
90	13.0
91	8.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016102432705987275
2	0.004689169963831459
3	4.423745248897603E-4
4	1.769498099559041E-4
5	0.0
6	4.423745248897603E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.847490497795205E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1130264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.27705481293947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75513745214579	27.082982745528504
2	14.679500090121236	11.237760591522408
3	5.796257538544724	6.655910025383702
4	2.7569207664919086	4.221072291757675
5	1.5473582941197244	2.9614159119638597
6	0.9118079904960102	2.0940794658655184
7	0.6209092393590414	1.6636603892164605
8	0.461966171747217	1.4146163582153628
9	0.34620153419272165	1.192641759055669
>10	1.7565881107380565	12.275596363619949
>50	0.16912079051066364	4.531645334947448
>100	0.16631922847437883	13.221299013967542
>500	0.020964608578622562	5.375613634281909
>1k	0.010948184479947339	6.071706114673995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2820	0.2494992320378248	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	2640	0.2335737491417934	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2629	0.23260052518703594	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2166	0.19163664418224416	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2107	0.18641662478854498	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2065	0.182700678779471	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1983	0.17544573657127893	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1839	0.16270535025445385	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1729	0.1529731107068791	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1719	0.15208836165709957	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1718	0.15199988675212164	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1648	0.14580664340366498	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	1628	0.14403714530410594	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	1457	0.12890793655287613	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1444	0.12775776278816275	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	1433	0.1267845388334053	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1416	0.1252804654487801	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	1412	0.1249265658288683	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	1393	0.12324554263428722	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	1375	0.12165299434468407	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1350	0.11944112172023527	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	1349	0.11935264681525733	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1347	0.11917569700530142	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	1277	0.11298245365684477	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1277	0.11298245365684477	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1274	0.11271702894191092	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	1269	0.11227465441702114	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	1230	0.10882413312288103	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	1213	0.10732005973825584	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1183	0.10466581258891727	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1176	0.10404648825407162	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	1175	0.10395801334909366	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	1146	0.10139224110473305	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	1145	0.1013037661997551	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	1139	0.10077291676988738	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	1132	0.10015359243504172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.847490497795205E-5	0.0
8	0.0	0.0	0.0	8.847490497795205E-5	0.0
9	0.0	0.0	0.0	8.847490497795205E-5	0.0
10	0.0	0.0	0.0	8.847490497795205E-5	0.0
11	0.0	0.0	0.0	2.6542471493385615E-4	0.0
12	0.0	0.0	0.0	2.6542471493385615E-4	0.0
13	0.0	0.0	0.0	2.6542471493385615E-4	0.0
14	0.0	0.0	0.0	2.6542471493385615E-4	0.0
15	0.0	0.0	0.0	2.6542471493385615E-4	0.0
16	0.0	0.0	0.0	3.538996199118082E-4	0.0
17	0.0	0.0	0.0	5.308494298677123E-4	0.0
18	0.0	0.0	0.0	9.732239547574726E-4	0.0
19	0.0	0.0	0.0	0.0010616988597354246	0.0
20	0.0	0.0	0.0	0.0013271235746692808	0.0
21	0.0	0.0	0.0	0.0016810231945810891	0.0
22	0.0	0.0	0.0	0.0022118726244488014	0.0
23	0.0	0.0	0.0	0.005220019393699172	0.0
24	1.769498099559041E-4	0.0	0.0	0.008670540687839302	0.0
25	1.769498099559041E-4	0.0	0.0	0.010616988597354247	0.0
26	1.769498099559041E-4	0.0	0.0	0.012917336126781	0.0
27	1.769498099559041E-4	0.0	0.0	0.01548310837114161	0.0
28	1.769498099559041E-4	0.0	0.0	0.02149940190964235	0.0
29	1.769498099559041E-4	0.0	0.0	0.032912664651798165	0.0
30	1.769498099559041E-4	0.0	0.0	0.06202090838954439	0.0
31	1.769498099559041E-4	0.0	0.0	0.12094519510486046	0.0
32	1.769498099559041E-4	0.0	0.0	0.2000417601551496	0.0
33	1.769498099559041E-4	0.0	0.0	0.27020235980266555	0.0
34	1.769498099559041E-4	0.0	0.0	0.35071452333260195	0.0
35	1.769498099559041E-4	0.0	0.0	0.44918709257306255	0.0
36	1.769498099559041E-4	0.0	0.0	0.579422152700608	0.0
37	1.769498099559041E-4	0.0	0.0	0.7877805539236851	0.0
38	1.769498099559041E-4	0.0	0.0	1.029670944133406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	40	7.031973E-4	27.499916	6
TAGGACC	1600	0.0	26.124922	4
ATAGGAC	300	0.0	24.199928	3
TTTCGCC	110	2.0190782E-10	23.999926	43
TAAGACC	85	2.3524444E-7	23.294046	4
AGGACCT	2355	0.0	22.420313	5
GTCCTAT	265	0.0	22.417011	1
TCCTACA	3510	0.0	22.063587	2
ACTGTTC	1400	0.0	21.999935	8
GGACCTG	2380	0.0	21.999933	6
TGTAGGA	3975	0.0	21.862537	2
CCTAGAG	225	0.0	21.511045	3
CTGTAGG	3810	0.0	21.482151	1
GATATAC	1640	0.0	21.33109	1
CCTACAG	3395	0.0	21.319525	3
ATACACT	1655	0.0	21.135887	4
TAGGACA	950	0.0	21.07362	4
GTCCTAC	3720	0.0	20.996418	1
CAGTGAT	1260	0.0	20.603111	37
TTAGGAC	2055	0.0	20.34057	3
>>END_MODULE