FastQCFastQC Report
Thu 2 Feb 2017
SRR4062485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336002
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG24830.7389836965256159No Hit
TCCATGTACTCTGCGTTGATACCAC20790.6187463169862084No Hit
GAGTACATGGAAGCAGTGGTATCAA10940.32559330003988074No Hit
CATGTACTCTGCGTTGATACCACTG9750.2901768441854513No Hit
GCTTCCATGTACTCTGCGTTGATAC7000.20833209326134966No Hit
CCCATGTACTCTGCGTTGATACCAC6320.18809411848738994No Hit
CATGGAAGCAGTGGTATCAACGCAG5550.1651775882286415No Hit
GCGTTGATACCACTGCTTCCATGTA5470.16279665001994037No Hit
GTACATGGGAAGCAGTGGTATCAAC5260.15654668722209988No Hit
GTATCAACGCAGAGTACATGGAAGC5120.15238004535687286No Hit
GTATCAACGCAGAGTACTTTTTTTT4680.1392848852090166No Hit
ACTCTGCGTTGATACCACTGCTTCC4370.1300587496502997No Hit
ACGCAGAGTACATGGAAGCAGTGGT3960.11785644133070637No Hit
CAGTGGTATCAACGCAGAGTACATG3940.11726120677853108No Hit
TATCAACGCAGAGTACATGGAAGCA3450.10267796025023662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA250.006017545719.002689
CCGACTT350.002163397816.28801212
CGACTTT350.002163397816.28801213
TTTCGTT350.002165551516.28558717
AGAACCG601.4585894E-615.8332085
AACCGCG551.1231459E-515.5453337
CGCGGTC508.6711225E-515.20214510
CATCGCC508.6711225E-515.20214515
GAATTTC456.74322E-414.77766218
ATCGCCA456.74322E-414.77766216
TTGGGCT456.75084E-414.7754624
GTCTTAG456.766102E-414.7710641
ATAGGAC653.3586039E-614.6130943
CGCATCG400.00526277914.2520113
CACTACC400.00526798314.24988718
TCACTAC400.00526798314.24988717
AGCTCGT400.00526798314.2498878
TAGAACC801.2806413E-714.24776654
TAGGACC801.2806413E-714.24776654
CAAGACG602.5602372E-514.2477664
Produced by FastQC (version 0.11.5)