##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062485_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336002
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.896539306313652	32.0	32.0	32.0	32.0	32.0
2	30.362170463271053	32.0	32.0	32.0	21.0	32.0
3	30.493794679793574	32.0	32.0	32.0	32.0	32.0
4	30.2207963047839	32.0	32.0	32.0	21.0	32.0
5	30.295084553068136	32.0	32.0	32.0	21.0	32.0
6	33.61134457532991	36.0	36.0	36.0	21.0	36.0
7	33.56840435473598	36.0	36.0	36.0	21.0	36.0
8	33.51261302016059	36.0	36.0	36.0	21.0	36.0
9	33.69516252879447	36.0	36.0	36.0	32.0	36.0
10	33.27505193421468	36.0	36.0	36.0	21.0	36.0
11	33.79139707501741	36.0	36.0	36.0	32.0	36.0
12	33.46275617407039	36.0	36.0	36.0	27.0	36.0
13	33.65674906696984	36.0	36.0	36.0	32.0	36.0
14	33.471196600020235	36.0	36.0	36.0	27.0	36.0
15	33.395461931774214	36.0	36.0	36.0	27.0	36.0
16	33.413054088963754	36.0	36.0	36.0	27.0	36.0
17	33.33337301563681	36.0	36.0	36.0	21.0	36.0
18	33.406554127654005	36.0	36.0	36.0	27.0	36.0
19	33.25017112993375	36.0	36.0	36.0	21.0	36.0
20	33.30698329176612	36.0	36.0	36.0	21.0	36.0
21	33.18776673948369	36.0	36.0	36.0	21.0	36.0
22	33.15890381604871	36.0	32.0	36.0	21.0	36.0
23	33.14682948315784	36.0	32.0	36.0	21.0	36.0
24	33.07174957291921	36.0	32.0	36.0	21.0	36.0
25	32.278516199308335	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	21.0
5	39.0
6	61.0
7	18.0
8	54.0
9	59.0
10	38.0
11	17.0
12	17.0
13	25.0
14	140.0
15	237.0
16	360.0
17	471.0
18	515.0
19	585.0
20	813.0
21	973.0
22	1280.0
23	1762.0
24	2445.0
25	3248.0
26	4727.0
27	6453.0
28	9009.0
29	12578.0
30	17210.0
31	24942.0
32	36540.0
33	50949.0
34	87627.0
35	72786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8345238945639	16.997940329071124	11.245148462985451	25.922387313379524
2	16.122206018036252	20.009226465073365	37.210631268787765	26.657936248102622
3	19.66533423418865	23.696562562318256	28.14270024436197	28.495402959131134
4	12.429452527802253	15.173659895696904	35.685114185697614	36.71177339080323
5	14.130295622969808	36.70679167646717	34.077774833490636	15.085137867072381
6	34.79082980224938	34.454347029869545	16.937887662397603	13.816935505483476
7	29.80509771919061	30.28877941850976	20.998457222165964	18.90766564013367
8	27.987845748246126	33.317842620313684	19.935356062975707	18.758955568464483
9	26.72057057906983	14.661903896514023	18.19465588588419	40.422869638531964
10	14.929616076856146	27.382981513045756	32.850399895109476	24.83700251498862
11	37.74993295390208	21.09925802318305	21.226794600554246	19.92401442236062
12	23.374599163159964	23.8475568324055	29.72927867249991	23.048565331934626
13	30.147383100706822	19.43156489504988	24.879614288949544	25.541437715293753
14	23.106057219823413	19.53555454239339	25.26081476117682	32.097573476606385
15	25.212597955960785	26.867792973987665	23.369983016000713	24.549626054050833
16	25.88122068805235	25.599945175111884	24.509704601119143	24.009129535716628
17	23.79754756619038	25.84203703979618	26.350999001743226	24.009416392270214
18	25.05759115495224	24.48302066725276	27.140707186601304	23.318680991193695
19	24.59850338392583	26.72034855479099	25.233268863412178	23.447879197871003
20	24.775984194481776	24.342107223634375	26.62115329028336	24.26075529160049
21	25.613968166931393	25.362450568149647	24.041983174545464	24.98159809037349
22	24.40050195386436	25.57967623379604	25.028540429045364	24.991281383294236
23	23.496031639590022	24.912004625531623	25.299750543172124	26.29221319170623
24	23.55186811025382	26.177682810167095	25.30181521249996	24.96863386707912
25	24.895025286314993	24.59850338392583	24.865224090094976	25.6412472396642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	28.0
2	99.5
3	171.0
4	171.0
5	171.0
6	367.0
7	563.0
8	563.0
9	563.0
10	656.0
11	749.0
12	749.0
13	749.0
14	818.0
15	887.0
16	887.0
17	887.0
18	1488.0
19	2089.0
20	2089.0
21	2089.0
22	3396.0
23	4703.0
24	4703.0
25	4703.0
26	6941.0
27	9179.0
28	9179.0
29	9179.0
30	11739.5
31	14300.0
32	14300.0
33	14300.0
34	17529.0
35	20758.0
36	20758.0
37	20758.0
38	23870.5
39	26983.0
40	26983.0
41	26983.0
42	31555.5
43	36128.0
44	36128.0
45	36128.0
46	44618.5
47	53109.0
48	53109.0
49	53109.0
50	49734.0
51	46359.0
52	46359.0
53	46359.0
54	41889.0
55	37419.0
56	37419.0
57	37419.0
58	33980.0
59	30541.0
60	30541.0
61	30541.0
62	26675.0
63	22809.0
64	22809.0
65	22809.0
66	18602.0
67	14395.0
68	14395.0
69	14395.0
70	10722.5
71	7050.0
72	7050.0
73	7050.0
74	5418.5
75	3787.0
76	3787.0
77	3787.0
78	3100.5
79	2414.0
80	2414.0
81	2414.0
82	1649.5
83	885.0
84	885.0
85	885.0
86	637.0
87	389.0
88	389.0
89	389.0
90	258.5
91	128.0
92	128.0
93	128.0
94	72.5
95	17.0
96	17.0
97	17.0
98	89.5
99	162.0
100	162.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007738049178278701
2	0.0035714073130517082
3	0.007440431902191059
4	0.017261802013083256
5	0.040178332271831714
6	0.052083023315337415
7	0.07261861536538473
8	0.09434467651978262
9	0.10208272569806132
10	0.12321355230028393
11	0.12261831774810865
12	0.13511824334378963
13	0.12321355230028393
14	0.12470163868072213
15	0.1166659722263558
16	0.11547550312200523
17	0.12410640412854686
18	0.13303492241117612
19	0.13243968785900084
20	0.1258921077850727
21	0.13065398420247498
22	0.15267766263296054
23	0.14077297158945482
24	0.1336301569633514
25	0.13243968785900084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	336002.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.5805615544475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.3201608577334	57.472447833781295
2	9.303386854152802	12.388494422155166
3	2.004527324900744	4.003876648293779
4	0.7259786335228512	1.9334426038592751
5	0.37516341068126025	1.2489295278920056
6	0.21860879877815803	0.8733057950035786
7	0.16034000774627696	0.7472869428774108
8	0.10678505275462549	0.5687847022419413
9	0.08532681477757112	0.5112996519188713
>10	0.5651143251899565	7.537319456469034
>50	0.0800492280314266	3.754328027238581
>100	0.050086667818527136	5.9523175186379
>500	0.003130416738657946	1.3240936424082206
>1k	0.0013416071737105484	1.6840732272230485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2483	0.7389836965256159	No Hit
TCCATGTACTCTGCGTTGATACCAC	2079	0.6187463169862084	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1094	0.32559330003988074	No Hit
CATGTACTCTGCGTTGATACCACTG	975	0.2901768441854513	No Hit
GCTTCCATGTACTCTGCGTTGATAC	700	0.20833209326134966	No Hit
CCCATGTACTCTGCGTTGATACCAC	632	0.18809411848738994	No Hit
CATGGAAGCAGTGGTATCAACGCAG	555	0.1651775882286415	No Hit
GCGTTGATACCACTGCTTCCATGTA	547	0.16279665001994037	No Hit
GTACATGGGAAGCAGTGGTATCAAC	526	0.15654668722209988	No Hit
GTATCAACGCAGAGTACATGGAAGC	512	0.15238004535687286	No Hit
GTATCAACGCAGAGTACTTTTTTTT	468	0.1392848852090166	No Hit
ACTCTGCGTTGATACCACTGCTTCC	437	0.1300587496502997	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	396	0.11785644133070637	No Hit
CAGTGGTATCAACGCAGAGTACATG	394	0.11726120677853108	No Hit
TATCAACGCAGAGTACATGGAAGCA	345	0.10267796025023662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	2.9761727608764235E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	25	0.0060175457	19.00268	9
CCGACTT	35	0.0021633978	16.288012	12
CGACTTT	35	0.0021633978	16.288012	13
TTTCGTT	35	0.0021655515	16.285587	17
AGAACCG	60	1.4585894E-6	15.833208	5
AACCGCG	55	1.1231459E-5	15.545333	7
CGCGGTC	50	8.6711225E-5	15.202145	10
CATCGCC	50	8.6711225E-5	15.202145	15
GAATTTC	45	6.74322E-4	14.777662	18
ATCGCCA	45	6.74322E-4	14.777662	16
TTGGGCT	45	6.75084E-4	14.775462	4
GTCTTAG	45	6.766102E-4	14.771064	1
ATAGGAC	65	3.3586039E-6	14.613094	3
CGCATCG	40	0.005262779	14.25201	13
CACTACC	40	0.005267983	14.249887	18
TCACTAC	40	0.005267983	14.249887	17
AGCTCGT	40	0.005267983	14.249887	8
TAGAACC	80	1.2806413E-7	14.2477665	4
TAGGACC	80	1.2806413E-7	14.2477665	4
CAAGACG	60	2.5602372E-5	14.247766	4
>>END_MODULE