FastQCFastQC Report
Thu 2 Feb 2017
SRR4062485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336002
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19380.5767822810578509No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16660.4958303819620122No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8020.23868905542228916No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7520.22380819161790705No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5920.17618942744388427No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4810.14315390979815595No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA4390.13065398420247498No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC4000.11904691043505694No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG3960.11785644133070637No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3530.10505889845893773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACATA301.2991617E-436.6665424
CGTAAAT250.00235042235.1998833
CCCTATA300.005735108629.3375991
GTGTACG300.005735108629.3375991
TAGGACG602.9347757E-729.3332334
CGCAATA1001.8189894E-1228.59990536
TACGAGC407.0216367E-427.49990714
GATTATA508.3156076E-526.4038411
CGAATGC1107.2759576E-1225.99991443
ATACGAA1107.2759576E-1225.99991440
CTTGCGC1151.2732926E-1124.8694823
GCAATAC1151.2732926E-1124.86948237
CGCCGGT1151.2732926E-1124.8694827
AATACGA1151.2732926E-1124.86948239
GACCGTG450.001396143324.4443636
AAACGGG450.001396143324.44436341
ATTATAC450.001396143324.4443633
AATAACG901.5043042E-824.4443632
GTCCTAT1102.0008883E-1024.003491
TAGATAG551.5903177E-423.999929
Produced by FastQC (version 0.11.5)