##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062485_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336002
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9794911935048	32.0	32.0	32.0	32.0	32.0
2	30.8274861459158	32.0	32.0	32.0	32.0	32.0
3	31.175826334367056	32.0	32.0	32.0	32.0	32.0
4	31.420902256534188	32.0	32.0	32.0	32.0	32.0
5	31.239697977988225	32.0	32.0	32.0	32.0	32.0
6	34.85265266278177	36.0	36.0	36.0	32.0	36.0
7	34.875328123046884	36.0	36.0	36.0	32.0	36.0
8	34.76886744721757	36.0	36.0	36.0	32.0	36.0
9	34.991330408747565	36.0	36.0	36.0	32.0	36.0
10	34.75640918804055	36.0	36.0	36.0	32.0	36.0
11	34.999851191361955	36.0	36.0	36.0	36.0	36.0
12	34.861735942047964	36.0	36.0	36.0	32.0	36.0
13	34.95681573323969	36.0	36.0	36.0	32.0	36.0
14	34.89713156469307	36.0	36.0	36.0	32.0	36.0
15	34.82631948619353	36.0	36.0	36.0	32.0	36.0
16	34.84311700525592	36.0	36.0	36.0	32.0	36.0
17	34.823566526389726	36.0	36.0	36.0	32.0	36.0
18	34.85651871119815	36.0	36.0	36.0	32.0	36.0
19	34.81634037892631	36.0	36.0	36.0	32.0	36.0
20	34.824620091547075	36.0	36.0	36.0	32.0	36.0
21	34.80234641460468	36.0	36.0	36.0	32.0	36.0
22	34.78779590597675	36.0	36.0	36.0	32.0	36.0
23	34.73318313581467	36.0	36.0	36.0	32.0	36.0
24	34.709778513223135	36.0	36.0	36.0	32.0	36.0
25	34.67460312736234	36.0	36.0	36.0	32.0	36.0
26	34.618540961065705	36.0	36.0	36.0	32.0	36.0
27	34.62377902512485	36.0	36.0	36.0	32.0	36.0
28	34.59215123719502	36.0	36.0	36.0	32.0	36.0
29	34.57304718424295	36.0	36.0	36.0	32.0	36.0
30	34.566261510348156	36.0	36.0	36.0	32.0	36.0
31	34.55408003523789	36.0	36.0	36.0	32.0	36.0
32	34.50619639168814	36.0	36.0	36.0	32.0	36.0
33	34.47845846155678	36.0	36.0	36.0	32.0	36.0
34	34.45667585311992	36.0	36.0	36.0	32.0	36.0
35	34.42047666382938	36.0	36.0	36.0	32.0	36.0
36	34.35725977821561	36.0	36.0	36.0	32.0	36.0
37	34.3668906732698	36.0	36.0	36.0	32.0	36.0
38	34.30036130737317	36.0	36.0	36.0	32.0	36.0
39	34.25405205921393	36.0	36.0	36.0	32.0	36.0
40	34.20831126005202	36.0	36.0	36.0	32.0	36.0
41	34.1921476657877	36.0	36.0	36.0	32.0	36.0
42	34.13636228355784	36.0	36.0	36.0	32.0	36.0
43	34.11642787840549	36.0	36.0	36.0	32.0	36.0
44	34.09927024243903	36.0	36.0	36.0	32.0	36.0
45	33.98950601484515	36.0	36.0	36.0	32.0	36.0
46	34.03865453181826	36.0	36.0	36.0	32.0	36.0
47	33.98787507217219	36.0	36.0	36.0	32.0	36.0
48	33.929592680995945	36.0	36.0	36.0	32.0	36.0
49	33.94399735715859	36.0	36.0	36.0	32.0	36.0
50	33.27187338170606	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	13.0
22	23.0
23	71.0
24	196.0
25	560.0
26	1094.0
27	2093.0
28	3667.0
29	6063.0
30	9506.0
31	14577.0
32	23220.0
33	40511.0
34	80247.0
35	154160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53767255799556	17.412061647627493	11.956579973450326	26.09368582092662
2	15.967356948553265	20.590003511925666	36.44099072018286	27.001648819338207
3	19.2091118505247	24.061166302581533	28.399830953387184	28.329890893506587
4	12.58415128481378	15.889786370319225	35.95157171683502	35.574490628031974
5	14.624912946946743	36.50305652942542	33.657240135475384	15.214790388152451
6	35.20159286188352	34.98522317030706	16.274453197777376	13.538730770032053
7	29.885238778340607	30.41350944339617	20.77815013005875	18.923101648204472
8	28.095368479949524	33.59474050749698	19.463277004303546	18.84661400824995
9	26.92156594305986	14.297533943250338	17.809715418360604	40.97118469532919
10	14.993065517467159	27.53852655638955	32.534925387348885	24.93348253879441
11	38.14768959708573	20.946899125600442	21.01207730906364	19.89333396825019
12	23.540336069428157	23.777834655746098	29.49922917125493	23.182600103570813
13	30.475711454098487	19.313277897155373	24.707293408967804	25.503717239778332
14	23.251647311623145	19.265361515705266	25.014434437890248	32.46855673478134
15	25.660263927000432	26.89626847459241	22.81534038487866	24.62812721352849
16	25.989131017077277	25.58347866976982	24.18378462032964	24.243605692823255
17	24.044797352396714	25.985559609764223	25.995678597151205	23.973964440687855
18	25.191814334438483	24.467413884441164	26.955196695257765	23.385575085862584
19	24.88586377462039	26.674245986631036	24.84806638055726	23.591823858191322
20	24.962946649127087	24.207296385140566	26.46353295516098	24.366224010571365
21	25.71294218486795	25.34746816983232	23.886762578794173	25.052827066505557
22	24.7266385319135	25.3239564050214	24.89300658924649	25.056398473818607
23	23.64093070874578	24.91562550222915	25.00818447509241	26.435259313932658
24	23.981404872590044	26.23883191171481	24.88973279920953	24.89003041648562
25	25.382289391134577	24.670686484009025	24.549556252641352	25.397467872215046
26	24.106701745822942	25.30788507211267	25.381991773858488	25.203421408205905
27	25.59240718805245	25.293897060136544	24.861161540705115	24.252534211105885
28	24.480211427312934	24.47544955089553	25.41740822971292	25.626930792078618
29	23.901643442598555	25.57008589234588	25.905500562496652	24.622770102558913
30	25.068303164862115	25.00699400598806	25.65579966785912	24.268903161290705
31	24.30253391348861	25.302825578419174	24.92485163778787	25.469788870304345
32	24.31116481449515	25.502824387950074	24.927232575996573	25.258778221558202
33	23.97455967524003	24.951934809911847	25.45490800649996	25.618597508348163
34	24.25342706293415	25.51294337533705	26.154903839858097	24.078725721870704
35	25.458181796536927	25.11592192903614	25.409372563258554	24.016523711168386
36	24.300748209832086	25.94984553663371	25.089731608740422	24.659674644793782
37	25.43853905631514	25.474253129445657	25.228123642121176	23.859084172118024
38	24.5724727829001	25.13139802739269	24.783483431646243	25.512645758060966
39	24.729614704674376	25.160564520449284	25.244492592306	24.86532818257034
40	25.614728483759023	25.303123195695264	24.540330117082636	24.541818203463077
41	23.65640680710234	25.379908452925875	26.041511657668703	24.922173082303082
42	26.232581948916973	25.72425164135928	24.72812661829394	23.31503979142981
43	25.13139802739269	24.10074940030119	25.063541288444714	25.704311283861404
44	24.704614853483015	24.961756180022736	25.779310837435492	24.554318129058757
45	25.202230939101554	25.35818239177148	25.061160350236012	24.37842631889096
46	24.340033690275654	25.038243819977264	25.858477032874806	24.76324545687228
47	25.35818239177148	24.419497502991053	26.107880310236247	24.114439795001218
48	25.04657710370772	26.498056559187148	24.352235998595244	24.10313033850989
49	24.065035327170673	25.11056481806656	26.274545984845325	24.549853869917442
50	24.37961678799531	26.309664823423667	23.998071440050953	25.31264694853007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	19.5
4	34.0
5	35.5
6	37.0
7	45.0
8	53.0
9	57.0
10	61.0
11	91.5
12	122.0
13	202.5
14	283.0
15	372.0
16	461.0
17	523.5
18	586.0
19	680.0
20	774.0
21	1005.5
22	1237.0
23	1419.5
24	1602.0
25	1910.5
26	2219.0
27	2843.0
28	3467.0
29	4020.0
30	4573.0
31	5088.0
32	5603.0
33	6429.5
34	7256.0
35	8272.5
36	9289.0
37	10869.0
38	12449.0
39	13386.5
40	14324.0
41	15607.0
42	16890.0
43	17529.5
44	18169.0
45	21365.0
46	24561.0
47	27593.0
48	30625.0
49	29815.0
50	29005.0
51	26476.0
52	23947.0
53	23226.5
54	22506.0
55	22614.0
56	22722.0
57	21888.5
58	21055.0
59	19074.0
60	17093.0
61	15164.5
62	13236.0
63	11380.5
64	9525.0
65	8241.0
66	6957.0
67	5941.0
68	4925.0
69	4460.0
70	3995.0
71	3110.0
72	2225.0
73	1952.5
74	1680.0
75	1229.5
76	779.0
77	703.5
78	628.0
79	519.5
80	411.0
81	324.5
82	238.0
83	213.0
84	188.0
85	143.0
86	98.0
87	77.0
88	56.0
89	40.5
90	25.0
91	20.0
92	15.0
93	10.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008333283730453986
2	0.0011904691043505692
3	0.0
4	0.0
5	0.0
6	8.928518282629271E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	336002.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.05619040943498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09477597176372	61.01528209144962
2	8.884348941686964	12.448072822453682
3	1.854462917619298	3.897498217919216
4	0.6987653244548455	1.958113464860771
5	0.3395369592537827	1.1893332934261784
6	0.20688816818235806	0.8696278142185491
7	0.13863451509994604	0.6798544191013056
8	0.09252398618278508	0.5185502394768898
9	0.07886220270938889	0.49723069402047665
>10	0.49842621730893644	6.911194145754507
>50	0.06936713851055568	3.3519297106644528
>100	0.041279830892715856	4.949037907761205
>500	0.001276695800805645	0.6397973102443099
>1k	8.511305338704299E-4	1.0744778686488783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1938	0.5767822810578509	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1666	0.4958303819620122	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	802	0.23868905542228916	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	752	0.22380819161790705	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	592	0.17618942744388427	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	481	0.14315390979815595	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	439	0.13065398420247498	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	400	0.11904691043505694	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	396	0.11785644133070637	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	353	0.10505889845893773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	2.9761727608764235E-4	0.0
26	0.0	0.0	0.0	2.9761727608764235E-4	0.0
27	0.0	0.0	0.0	2.9761727608764235E-4	0.0
28	0.0	0.0	0.0	5.952345521752847E-4	0.0
29	0.0	0.0	0.0	5.952345521752847E-4	0.0
30	0.0	0.0	0.0	0.0011904691043505694	0.0
31	0.0	0.0	0.0	0.0035714073130517082	0.0
32	0.0	0.0	0.0	0.008035666454366343	0.0
33	0.0	0.0	0.0	0.013392777423943906	0.0
34	0.0	0.0	0.0	0.02023797477395968	0.0
35	0.0	0.0	0.0	0.03035696216093952	0.0
36	0.0	0.0	0.0	0.04523782596532164	0.0
37	0.0	0.0	0.0	0.07053529443277123	0.0
38	0.0	0.0	0.0	0.10833268849590182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACATA	30	1.2991617E-4	36.666542	4
CGTAAAT	25	0.002350422	35.199883	3
CCCTATA	30	0.0057351086	29.337599	1
GTGTACG	30	0.0057351086	29.337599	1
TAGGACG	60	2.9347757E-7	29.333233	4
CGCAATA	100	1.8189894E-12	28.599905	36
TACGAGC	40	7.0216367E-4	27.499907	14
GATTATA	50	8.3156076E-5	26.403841	1
CGAATGC	110	7.2759576E-12	25.999914	43
ATACGAA	110	7.2759576E-12	25.999914	40
CTTGCGC	115	1.2732926E-11	24.869482	3
GCAATAC	115	1.2732926E-11	24.869482	37
CGCCGGT	115	1.2732926E-11	24.869482	7
AATACGA	115	1.2732926E-11	24.869482	39
GACCGTG	45	0.0013961433	24.444363	6
AAACGGG	45	0.0013961433	24.444363	41
ATTATAC	45	0.0013961433	24.444363	3
AATAACG	90	1.5043042E-8	24.444363	2
GTCCTAT	110	2.0008883E-10	24.00349	1
TAGATAG	55	1.5903177E-4	23.99992	9
>>END_MODULE