FastQCFastQC Report
Thu 2 Feb 2017
SRR4062484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526249
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG27610.18090101942736736No Hit
TCCATGTACTCTGCGTTGATACCAC23690.155217136915405No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20410.13372654134417122No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20200.13235061906674467No Hit
GCGCAAGACGGACCAGAGCGAAAGC20090.13162989787380697No Hit
GAATAGGACCGCGGTTCTATTTTGT18780.12304676366700323No Hit
GTATCAACGCAGAGTACTTTTTTTT17920.11741203434039924No Hit
GAATAACGCCGCCGCATCGCCAGTC17640.1155774713038305No Hit
GGGTAGGCACACGCTGAGCCAGTCA16820.11020482241102206No Hit
GTCCTATTCCATTATTCCTAGCTGC16730.1096151414349821No Hit
GAACTACGACGGTATCTGATCGTCT16430.10764953818151558No Hit
GTATCTGATCGTCTTCGAACCTCCG15410.10096648711972948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTT402.7610004E-416.62505711
CGAACGA851.0168151E-915.65019416
ATGTACG551.12579055E-515.54907717
GGCGTAC551.1338685E-515.5373531
GTTTTCG1850.014.89389715
TTTTCGG2050.013.90522416
GCGTTAT1101.8735591E-1013.810981
CGTTATT1051.380613E-913.56482
TTTCGGA2050.013.44215817
GGTTCTA4000.013.06383113
ACGAACG1051.9894287E-812.66837215
ACGATCA1155.316906E-912.392169
GCGGTAA853.9314655E-612.29738719
CGGTTCT3950.012.26626812
TTTACGA550.003066813612.09174310
CGCAAGA3250.011.9787632
AGGCCCG3700.011.811625510
CGCGGTA1459.640644E-1111.79585418
GAACCGC1459.640644E-1111.7935316
GTATTAG3500.011.6653461
Produced by FastQC (version 0.11.5)